Hostname: page-component-76fb5796d-vvkck Total loading time: 0 Render date: 2024-04-26T12:19:10.652Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes

Published online by Cambridge University Press:  26 September 2008

S. Ledda ,
P. Loi ,
L. Bogliolo ,
R.M. Moor  and
J. Fulka Jr
S. Ledda
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
P. Loi
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
L. Bogliolo
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
R.M. Moor
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
J. Fulka Jr*
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
*
J. Fulka, Jr, Institute of Animal Production, CS-104 00 Prague 10 – Uhříněves, Czech Republic. Telephone: 42 267710659. Fax: 42 267710779.
Get access Rights & Permissions [Opens in a new window]

Summary

Mouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethyl-aminopurine (6-DMAP) and thereafter activated with ethanol before further culture in 6-DMAP supplemented medium. The presence of 6-DMAP enhances the efficiency of activation and the speed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showing that the preincubation of condensed chromosomes with 6-DMAP blocks DNA synthesis when nuclei become reformed.

Keywords

ActivationDNA synthesisOocytes
Type
Article
Information
Zygote , Volume 4 , Issue 1 , February 1996 , pp. 7 - 9
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Blow, J.J. (1993). Preventing re-replication of DNA in a single cell cycle: evidence for a Replication Licensing factor. J. Cell Biol. 122, 993–1002.CrossRefGoogle Scholar
Chong, J.P.J., Mahbubani, H.M., Khoo, C.Y. & Blow, J.J. (1995). Purification of an MCM-containing complex as a component of the DNA replication licensing system. Nature 375, 418–21.CrossRefGoogle ScholarPubMed
Kubota, Y. & Takisawa, H. (1993). Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts. J. Cell Biol. 123, 1321–31.CrossRefGoogle ScholarPubMed
Madine, M.A., Khoo, C.Y., Mills, A.D. & Laskey, R.A. (1995). MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells. Nature 375, 421–4.CrossRefGoogle ScholarPubMed
Moses, R.M., Kline, D. & Masui, Y. (1995). Maintenance of metaphase in colcemid-treated mouse eggs by distinct calcium and 6-dimethylaminopurine (6-DMAP)-sensitive mechanisms. Dev. Biol. 167, 329–37.CrossRefGoogle ScholarPubMed
Moses, R.M. & Masui, Y. (1994). Enhancement of mouse egg activation by the kinase inhibitor, 6-dimethylaminopurine (6-DMAP). J. Exp. Zool. 270, 211–18.CrossRefGoogle ScholarPubMed
Ouhibi, N., Fulka, J. Jr, Kaňka, J. & Moor, R.M. (1994). A reversible block at the G1/S border during cell cycle progression in mouse embryos. Int. J. Dev. Biol. 38, 731–6.Google ScholarPubMed
Staigmiller, R.B. & Moor, R.M. (1984). Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicle. Gamete Res. 9, 221–9.CrossRefGoogle Scholar
Susko-Parish, J.L., Leibfried-Rutledge, M.L., Northey, D.L., Schutzkus, V. & First, N.L. (1994). Inhibition of protein kinases after an induced calcium transient cause transition of bovine oocytes to embryonic cycles without meiotic completion. Dev. Biol. 166, 729–39.CrossRefGoogle Scholar
Szöllösi, M.S., Kubiak, J.Z., Debey, P., De Pennart, H., Szöllösi, D. & Maro, B. (1993). Inhibition of protein kinases by 6-dimethylaminopurine accelerates the transition to interphase in activated mouse oocytes. J. Cell Sci. 104, 861–72.CrossRefGoogle ScholarPubMed
Thibault, C., Szöllösi, D. & Gerard, M. (1987). Mammalian oocyte maturation. Reprod. Nutr. Dev. 27, 865–96.CrossRefGoogle ScholarPubMed
Yan, H., & Newport, J. (1995). An analysis of the regulation of DNA synthesis by cdk2, Cip1, and Licensing Factor. J. Cell Biol. 129, 1–15.CrossRefGoogle ScholarPubMed