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During capacitation in bull spermatozoa, actin and PLC-ζ undergo dynamic interactions

Published online by Cambridge University Press:  20 September 2017

Itzayana Mejía-Flores ,
Natalia Chiquete-Félix ,
Icela Palma-Lara ,
Salvador Uribe-Carvajal  and
María de Lourdes Juárez-Mosqueda
Itzayana Mejía-Flores
Affiliation:
Departamento de Morfología, Facultad de Medicina Veterinaria y Zootecnia, UNAM
Natalia Chiquete-Félix
Affiliation:
Instituto de Fisiología Celular, UNAM, C.U., CDMX, 04510, México.
Icela Palma-Lara
Affiliation:
Escuela Superior de Medicina, IPN, Plan de San Luis y Díaz Mirón, Col. Santo Tomás, CDMX, 11340, México.
Salvador Uribe-Carvajal
Affiliation:
Instituto de Fisiología Celular, UNAM, C.U., CDMX, 04510, México.
María de Lourdes Juárez-Mosqueda*
Affiliation:
Departamento de Morfología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, C.U., CDMX, 04510, México.
*
All correspondence to: María de Lourdes Juárez-Mosqueda. Departamento de Morfología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, C.U., CDMX, 04510, México. Tel:/Fax: +52 55 56225910. E-mail: luerfco@unam.mx
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Summary

The migration pattern of sperm-specific phospholipase C-ζ (PLC-ζ) was followed and the role of this migration in actin cytoskeleton dynamics was determined. We investigated whether PLC-ζ exits sperm, opening the possibility that PLC-ζ is the ‘spermatozoidal activator factor’ (SOAF). As capacitation progresses, the highly dynamic actin cytoskeleton bound different proteins to regulate their location and activity. PLC-ζ participation at the start of fertilization was established. In non-capacitated spermatozoa, PLC-ζ is in the perinuclear theca (PT) and in the flagellum, therefore it was decided to determine whether bovine sperm actin interacts with PLC-ζ to direct its relocation as it progresses from non-capacitated (NC) to capacitated (C) and to acrosome-reacted (AR) spermatozoa. PLC-ζ interacted with actin in NC spermatozoa (100%), PLC-ζ levels decreased in C spermatozoa to 32% and in AR spermatozoa to 57% (P < 0.001). The level of actin/PLC-ζ interaction was twice as high in G-actin (P < 0.001) that reflected an increase in affinity. Upon reaching the AR spermatozoa, PLC-ζ was partially released from the cell. It was concluded that actin cytoskeleton dynamics control the migration of PLC-ζ during capacitation and leads to its partial release at AR spermatozoa. It is suggested that liberated PLC-ζ could reach the egg and favour fertilization.

Keywords

ActinBull sperm capacitationPLC-ζProtein–protein interactionSOAF
Type
Research Article
Information
Zygote , Volume 25 , Issue 5 , October 2017 , pp. 558 - 566
Copyright
Copyright © Cambridge University Press 2017 

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