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Dimethyl sulphoxide and electrolyte-free medium improve exogenous DNA uptake in mouse sperm and subsequently gene expression in the embryo

Published online by Cambridge University Press:  31 October 2018

Soleiman Kurd ,
Sara Hosseini ,
Fardin Fathi ,
Vahid Jajarmi  and
Mohammad Salehi Open the ORCID record for Mohammad Salehi [Opens in a new window]
Soleiman Kurd
Affiliation:
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Sara Hosseini
Affiliation:
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Fardin Fathi
Affiliation:
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
Vahid Jajarmi
Affiliation:
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mohammad Salehi*
Affiliation:
Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
*
*Address for correspondence: Mohammad Salehi. P.O. Box: 193954717, Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. E-mail: m.salehi@sbmu.ac.ir
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Summary

One of the methods to generate transgenic animals is called sperm-mediated gene transfer (SMGT). Mature sperm cells can take up exogenous DNA molecules intrinsically and transfer them into the oocyte during fertilization. This study assessed the effect of dimethyl sulfoxide (DMSO) and electrolyte-free medium (EFM) on DNA uptake (EGFP–N1plasmid) in mouse sperm. Sperms cells cultured in human tubular fluid (HTF) without any treatment were considered as the control group. Sperms cells that were incubated in EFM and HTF with DNA/DMSO at 4°C were classified into EFM and HTF groups. Sperm motility and viability were assessed following treatment. In vitro fertilization (IVF) with sperm in all groups was performed. Fertilization, embryo development and GFP-positive blastocyst rates were analyzed and compared. The result showed that sperm motility and viability in EFM were better than those in the HTF group. The rate of development to reach the blastocyst stage and GFP-positive blastocysts was significantly higher in the EFM group compared with the HTF group (P<0.05). Our data demonstrate that sperm stored in the EFM group can improve the efficiency of SMGT for the generation of GFP-positive blastocysts.

Keywords

DMSOElectrolyte-free mediumGreen fluorescent protein (GFP)Sperm-mediated gene transferSpermatozoa
Type
Research Article
Information
Zygote , Volume 26 , Issue 5 , October 2018 , pp. 403 - 407
Copyright
© Cambridge University Press 2018 

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References

Alderson, J, Wilson, B, Laible, G, Pfeffer, P L’Huillier, P (2006) Protamine sulfate protects exogenous DNA against nuclease degradation but is unable to improve the efficiency of bovine sperm mediated transgenesis. Anim Reprod Sci 91, 23–30.Google Scholar
Björndahl, L, Söderlund, I Kvist, U (2003) Evaluation of the one‐step eosin‐nigrosin staining technique for human sperm vitality assessment. Hum Reprod 18, 813–816.Google Scholar
Brackett, B, Baranska, W, Sawicki, W Koprowski, H (1971) Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. Proc Natl Acad Sci USA 68, 353–357.Google Scholar
Cooper, TG Yeung, C-H (2006) Computer-aided evaluation of assessment of ‘grade a’ spermatozoa by experienced technicians. Fertil Steril 85, 220–224.Google Scholar
Furuta, H, Ichikawa, E, Sugimura, S, Kikuchi, S, Yoshida, T, Mukouyama, H Tomogane, H (2006) Gene transfer to mouse embryos by sperm mediated gene transfer method. J App Anim Res 29, 113–116.Google Scholar
Hassanane, MS, El Makawy, AI, Helalia, SM, Abdoon, AS, Khalil, KMA, Ghanem, TA, Tohamy, AM, Sun, X-F Shen, W (2017) First study of sperm mediated gene transfer in Egyptian river buffalo. J Gen Eng Biotechnol 15, 475–482.Google Scholar
Hosseini, S, Dehghani-Mohammadabadi, M, Ghafarri Novin, M, Haji Molla Hoseini, M, Arefian, E, Mohammadi Yeganeh, S Salehi, M (2017) Toll-like receptor 4 as a modulator of fertilization and subsequent pre-implantation development following in vitro maturation in mice. Am J Reprod Immunol 78, doi: 10.1111/aji.12720.Google Scholar
Khoo, HW (2000) Spermmediated gene transfer studies on zebrafish in Singapore. Mol Reprod Dev 56, 278–280.Google Scholar
Lavitrano, M, Bacci, ML, Forni, M, Lazzereschi, D, Di Stefano, C, Fioretti, D, Giancotti, P, Marfé, G, Pucci, L, Renzi, L, Wang, H, Stoppacciaro, A, Stassi, G, Sargiacomo, M, Sinbaldi, P, Turchi, V, Giovannoni, R, Della Cassa, G, Seren, E Rossi, G. (2002) Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation. Proc Natl Acad Sci USA 99, 14230–14235.Google Scholar
Lavitrano, M, Busnelli, M, Cerrito, MG, Giovannoni, R, Manzini, S Vargiolu, A (2005) Sperm-mediated gene transfer. Reprod Fertil Dev 18, 19–23.Google Scholar
Lavitrano, M, Maione, B, Forte, E, Francolini, M, Sperandio, S, Testi, R Spadafora, C (1997) The interaction of sperm cells with exogenous DNA: a role of CD4 and major histocompatibility complex class II molecules. Exp Cell Res 233, 56–62.Google Scholar
Lillico, S, Sherman, A, McGrew, MJ, Robertson, CD, Smith, J, Haslam, C, Barnard, P, Radcliffe, PA, Mitrophanous, KA, Elliot, EA Sang, HM (2007) Oviduct-specific expression of two therapeutic proteins in transgenic hens. Proc Natl Acad Sci USA 104, 1771–1776.Google Scholar
Love, J, Gribbin, C, Mather, C Sang, H (1994) Transgenic birds by DNA microinjection. Nat Biotechnol 12, 60–63.Google Scholar
Nakanishi, A Iritani, A (1993) Gene transfer in the chicken by sperm‐mediated methods. Mol Reprod Dev 36, 258–261.Google Scholar
Parrington, J, Coward, K Gadea, J (2011) Sperm and testis mediated DNA transfer as a means of gene therapy. Sys Biol Reprod Med 57, 35–42.Google Scholar
Riel, JM, Yamauchi, Y, Huang, TT, Grove, J Ward, MA (2011) Short-term storage of human spermatozoa in electrolyte-free medium without freezing maintains sperm chromatin integrity better than cryopreservation. Biol Reprod 85, 536–547.Google Scholar
Saito, K, Kinoshita, Y, Kanno, H, Iwasaki, A Hosaka, M (1996) A new method of the electrolyte-free long-term preservation of human sperm at 4°C. Fertil Steril 65, 1210–1213.Google Scholar
Salehi, M, Kato, Y Tsunoda, Y (2014) Effect of melatonin treatment on developmental potential of somatic cell nuclear-transferred mouse oocytes in vitro . Zygote 22, 213–217.Google Scholar
Sato, M. (2005) Transgenesis via sperm. J Mammal Ova Res 22, 92–100.Google Scholar
Shen, W, Li, L, Pan, Q, Min, L, Dong, H Deng, J (2006) Efficient and simple production of transgenic mice and rabbits using the new DMSO‐sperm mediated exogenous DNA transfer method. Mol Reprod Dev 73, 589–594.Google Scholar
Smith, K Spadafora, C (2005) Sperm‐mediated gene transfer: applications and implications. Bioessays 27, 551–562.Google Scholar
Van de Lavoir, M-C, Diamond, JH, Leighton, PA, Mather-Love, C, Heyer, BS, Bradshaw, R, Kerchner, A, Hooi, LT, Gessaro, TM, Swanberg, SE, Delany, ME Etches, RJ (2006) Germline transmission of genetically modified primordial germ cells. Nature 441, 766.Google Scholar
Zani, M, Lavitrano, M, French, D, Lulli, V, Maione, B, Sperandio, S Spadafora, C (1995) The mechanism of binding of exogenous DNA to sperm cells: factors controlling the DNA uptake. Exp Cell Res 217, 57–264.Google Scholar