Skip to main content Accessibility help
×
Home

Different calcium-dependent pathways control fertilisation-triggered glycoside release and the cortical contraction in ascidian eggs

  • Alex McDougall (a1), Christian Sardet (a1) and Charles. C Lambert (a1)

Summary

Fertilisation of ascidian eggs induces the rapid release of a cell surface N-acetylglycosaminidase that blocks sperm binding to vitelline coat sperm receptors resulting in a block to polyspermy. Fertilisation also triggers a large contraction of the egg (thus stimulating ooplasmic segregation) that is completed within 5 min of insemination. In eggs of the ascidian Phallusia mammillata, glycosidase release and cortical contractions are blocked by BAPTA-AM [bis-(o-aminophenoxy-ethane-N,N,N',N' -tetraacetic acid, tetra(acetoxymethyl)-ester], a cell-permeant calcium chelator, indicating that both processes are probaly dependent on a rise in intracellular calcium levels. Both glycosidase release and the cortical contraction are induced by treatment of the egg with the protein synthesis inhibitor emetine, while only the glycosidase release is induced by isoproterenol, carbachol or acetylcholine. Previous work with ryanodine demonstrated that ryanodine also caused glycosidase release but not the cortical contraction Inversely, activation by ionomycin in calcium-free sea water causes cortical contractions but not glycosidase release. Thus the two processes can be activated independently. Dextran-coupled (10kDa) calcium green-1 injected eggs show an increase in intracellular calcium 30–40s before the cortical contraction is triggered by fertilisation or ionomycin- induced activation. This confirms previous findings that the cortical contraction is a consequence of the activation calcium the triggered by te sperm. The extracellular calcium requirement for the glycosidase release suggests that calcium influx may be more important for this phase of egg activation. Thus activation eggs appears to involve two independent pathways involving calcium.

Copyright

Corresponding author

Alex McDougall, URA 671 CNRS/Paris VI, Station Zoologique, F-06230 Villefranche-sur-Mer, France. Fax: 33.93.76.37.92.

References

Hide All
Abdelmajid, H.Leclerc-David, C.Moreau, M.Guerrier, P. & Ryazanov, A. (1993). Release from the metaphase I block in invertebrate oocytes possible involvement of Ca2+/calmodulin-dependent kinase III. Int.J. Dev. Biol. 37 279–90.
Arnoult, C.Georges, D. & Villaz, M. (1994). Cell cycle-related fluctuations in oocyte surface area of the ascidian Ciona intestinalis after meiosis resumption. Dev. Biol. 166 110.
Berridge, M.J. (1993). Inositol triphosphate and calcium signaling Nature 361, 315–25.
Bezprovanny, I.Watras, J. & Ehrlich, B.E. (1991). Bell-shaped calcium- response curves of Ins(1,4,5,)P3- and calciumgated channels from endoplasmic reticulum of cerebellum Nature 351, 751–4.
Colas, P.Launay, C.Van Loon, A. & Guerrier, P. (1993) Protein synthesis controls cyclin stability im metaphase I-arrested oocytes of Patella vulgata Exp. Cell Res. 208 518–21.
Dale, B. (1988) Primary and secondary messengers in the activation of ascidian eggs. Exp. Cell Res. 177 215–11.
Dasilva, A.M. & Klein, C. (1989). Characterization of a glycosyl-phosphatidylinositol degarding actvity in Dicytostelium discoideum membranes Exp. Cell Res. 185 464–72.
Galione, A. & White, A. (1994). Ca++ release induced by cyclic ADP-ribose Trends Cell Biol. 4 436–6.
Galione, A.McDougall, A.Busa, W.BWillmott, N.Gilott, I. & Whitaker, M. (1993) Redundant mechanisms of calcium-induced calcium release underlying calcium waves during fertilization of sea urchin eggs. Science. 261, 348–52.
Goudeau, M. & Goudeau, H. (1993). In the egg of Phallusia mammallata, removal of external calcium modifies fertilization potential, induces polyspermy, and blocks the resumption of meiosis. Dev. Biol. 160, 165–77.
Hille, B. (1984). Ionic Channels of Excitable Membranes. SunderlandMA: Sinauer Associates.
Kawahara, H. & Yokosawa, H. (1994). Intracellular calcium calcium mobilization regulates the activity of 26 S proteosome during the metaphase-anaphase transition in the ascidian cell cycle Dev. Biol. 166 623–33.
Kiehart, D.P. (1982). Microinjection of echinoderm eggs. In Methods Cell Biol. 25 1331.
Kline, D. & Kline, J.T. (1992) Thapsigargin activates a calcium influx pathway in the unfertilized mouse egg and suppresses repetitive calcium transients in the fertilized eggs. J. Biol. Chem. 267 17624–30.
Lambert, C.C. (1989). Ascidian eggs release glycosidase activity which aids in the block against polyspermy. Development 105, 415–20.
Lambert, C.C. & Goode, C.A. (1992.) Glycolipid linkage of a polyspermy blocking glycosidase to the ascidian egg surface. Dev. Biol. 153 95100.
Lambert, C.C.Gonzalez, G.P. & Millar, K.M. (1994). Independent initiation of calcium dependent glycosidase release and cortical contractions during the activation of ascidian eggs. Dev. Growth Differ. 36 133–9.
Lee, H.C.Aarhus, R. & Walseth, T.F. (1993). Calcium mobilization by dual receptors during fertilization of sea urchin eggs. Science 261, 352–5.
Marks, P.W. & Maxfield, F.R. (1990). Transient increases in cytosolic free calcium appear to be required for the migration of adherent human neutrophils. J.Cell Biol. 110 4352.
McDougall, A. & Sardet, C. (1995). Function and characteristics of repetitive calcium waves associated with meiosis. Curr.Biol. 5, 318–28.
McDougall, A.D.Gillot, I. & Whitaker, M. (1993). Thimerosal reveals calcium-induced calcium release in unfertilised sea urchin eggs. Zygote 1, 3543.
Miyazaki, S.Katayama, Y. & Swann, K. (1990) Synergistic activation by serotonin and GTP analogue and inhibition by phorbol ester of cyclic Ca2+ rises in hamster eggs. J. physiol.(Lond) 426 209–27.
Nuccitelli, R. (1991). How do sperm activate eggs? Curr. Top. Dev. Biol. 25 116.
Nuccitelli, R.Yim, D.L. & Smart, T. (1993). The sperm induced Ca++ wave following fertilization in Xenopus eggs requires the production of Ins(1,4,5)P3 Dev. Biol. 158 200–12.
Putney, J.W. (1993). Excitement about calcium signaling in unexcitable cells. Science 262 676–78.
Sardet, C.Speksnijder, J.Inoué, S. & Jaffe, L. (1989). Fertilization and ooplasmic movements in the ascidian egg. Development 105 237–50.
Speksnijder, J.Corson, D.W.Sardet, C. &Jaffe, L.F. (1989). Free calcium pulses following fertilization in the ascidian egg. Dev. Biol. 135 182–90.
Speksnijder, J.E.Sardet, C. & Jaffe, L.F. (1990). The activation wave of calcium in the ascidian egg and its role in ooplasmic segregation. J. Cell Biol. 110 1589–98.
Swann, K. (1991). Thimerosol causes calcium oscillations and sensitizes calcium-induced calcium release in unfertilized hamster eggs. FEBS Lett 278 175–8.
Swann, K. & Whitaker, M. (1986). The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchins. J. Cell. Biol. 103 2333–42.
Swann, K.McDougall, A. & Whitaker, M. (1994). Calcium signaling at fertilization J. Mar. Biol. Assoc. U.k. 74 316.
Ting, A.E. & Pogano, R.E. (1990). Detection of a phosphati-dylinositol-specific phospholipase C at the surface of Swiss 3T3 cells and its potential role in the regulation of cell growth. J. Biol. chem 265 5337–40.
Ting, A.E. & Pogano, R.E. (1991). Density dependent inhibition of cell growth is correlated with the activity of a phosphatidyl- inositol specific phospholipase C. Eur. J. Cell Biol. 56 401–6.
Volwerk, J.J.Birrell, G.B.Hedberg, K.K. &Griffith, O.H. (1992) A high level of cell-surface phosphatidylinositol- specific phospholipase—activity is characterristic of growth arrested 3T3 fibroblasts but not of transformed variants. J. cell. physiol. 151 613–22.
Whitaker, M. & Swann, K. (1993). Lighting the fuse at fertilization. Development 117 112.

Keywords

Different calcium-dependent pathways control fertilisation-triggered glycoside release and the cortical contraction in ascidian eggs

  • Alex McDougall (a1), Christian Sardet (a1) and Charles. C Lambert (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed