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Cyclopiazonic acid induces accelerated progress of meiosis in pig oocytes

Published online by Cambridge University Press:  26 September 2008

Jaroslav Petr ,
Jirří Rozinek  and
František Jílek
Jaroslav Petr*
Affiliation:
Research Institute of Animal Production and Czech University of Agriculture, Prague, Czech Republic
Jirří Rozinek
Affiliation:
Research Institute of Animal Production and Czech University of Agriculture, Prague, Czech Republic
František Jílek
Affiliation:
Research Institute of Animal Production and Czech University of Agriculture, Prague, Czech Republic
*
Ing. Jaroslav Petr DrSc, Research Institute of Animal Production, 104 00 Prague 10–Uhříněves, Czech Republic. Tel: +42 2 67711747. Fax: +42 2 67710779.
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Summary

In mammalian oocytes, calcium plays an important role in the regulation of meiotic maturation. In our study, we used the mycotoxin cyclopiazonic acid (CPA), an inhibitor of calcium-dependent ATPases, to mobilise intracellular calcium deposits during in vitro maturation of pig oocytes. The CPA treatment of maturing oocytes significantly accelerated the progress of their maturation. Oocytes entered the CPA-sensitive period after 21 h of in vitro culture. A very short (5 min) exposure to CPA (100 mM) is sufficient to accelerate maturation and it seems that accelerated maturation can be triggered by a transient elevation of intracellular calcium levels. The effect of CPA is not mediated through the cumulus cells, because maturation is accelerated by CPA treatment even in oocytes devoid of cumulus cells. Culture of oocytes with the calcium channel blocker verapamil (concentrations ranging from 0.01 to 0.04 mM) blocked the progress of oocyte maturation beyond the stage of metaphase I. This block can be overcome by the mobilisation of intracellular calcium deposits after CPA treatment (100 nM). The microinjection of heparin (20 pl, 50.1 mg/;ml), the inhibitor of inositol triphosphate receptors, before CPA treatment prevented the acceleration of oocyte maturation. This indicates that CPA mobilises the release of calcium deposits through inositol trisphosphate receptors. On the other hand, the microinjection of procaine (20 pl, 200 nM) or the microinjection of ruthenium red (20 pl, 50 mM), both inhibitors of ryanodine receptors, did not prevent accelerated maturation in CPA-treated oocytes. If present in pig oocytes, ryanodine receptors evidently play no part in the liberation of calcium from intracellular stores after CPA treatment.

Keywords

CalciumCyclopiazonic acidMaturationOocytePig
Type
Article
Information
Zygote , Volume 5 , Issue 3 , August 1997 , pp. 193 - 205
Copyright
Copyright © Cambridge University Press 1997

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