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CsA promotes trophoblast invasion accompanied by changes in leukaemic inhibitory factor and fibroblast growth factor in peri-implantation blastocysts

Published online by Cambridge University Press:  21 December 2023

Dan Li Open the ORCID record for Dan Li [Opens in a new window] ,
Qiuling Jie ,
Qi Li ,
Ping Long ,
Zhen Wang ,
Jiaxing Wang ,
Shengnan Tian ,
Menglan Wu ,
Yanlin Ma  and
Yuanhua Huang
Dan Li
Affiliation:
Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Hainan Medical University, China Department of Reproductive Medicine, Haikou Women & Children Hospital, China
Qiuling Jie
Affiliation:
Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Hainan Medical University, China Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China
Qi Li
Affiliation:
Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China
Ping Long
Affiliation:
Guizhou Qiannan People’s Hospital, China
Zhen Wang
Affiliation:
Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China
Jiaxing Wang
Affiliation:
Hainan Medical University, China
Shengnan Tian
Affiliation:
Hainan Medical University, China
Menglan Wu
Affiliation:
Department of Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, China
Yanlin Ma
Affiliation:
Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Hainan Medical University, China Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China
Yuanhua Huang*
Affiliation:
Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Hainan Medical University, China Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, China
*
Corresponding author: Yuanhua Huang; Email: 13036095796@163.com
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Summary

During the early stages of human pregnancy, successful implantation of embryonic trophoblast cells into the endometrium depends on good communication between trophoblast cells and the endometrium. Abnormal trophoblast cell function can cause embryo implantation failure. In this study, we added cyclosporine A (CsA) to the culture medium to observe the effect of CsA on embryonic trophoblast cells and the related mechanism. We observed that CsA promoted the migration and invasion of embryonic trophoblast cells. CsA promoted the expression of leukaemic inhibitory factor (LIF) and fibroblast growth factor (FGF). In addition, CsA promoted the secretion and volume increase in vesicles in the CsA-treated group compared with the control group. Therefore, CsA may promote the adhesion and invasion of trophoblast cells through LIF and FGF and promote the vesicle dynamic process, which is conducive to embryo implantation.

Keywords

Cyclosporine AFibroblast growth factorInvasionLeukaemic inhibitory factorTrophoblast
Type
Research Article
Information
Zygote , Volume 32 , Issue 1 , February 2024 , pp. 71 - 76
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally.

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