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CRMP5 participates in oocyte meiosis by regulating spastin to correct microtubule–kinetochore misconnection

Published online by Cambridge University Press:  04 December 2023

Zhen Jin ,
Zhi-Cai Zhang ,
Chen-Yu Xiao ,
Mei-Qi Li ,
Qian-Ru Li  and
Lei-Lei Gao Open the ORCID record for Lei-Lei Gao [Opens in a new window]
Zhen Jin
Affiliation:
Center for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China
Zhi-Cai Zhang
Affiliation:
Department of Dispatching Management, Zibo Medical Emergency Command Center, Zibo, Shandong, 255030, China
Chen-Yu Xiao
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China
Mei-Qi Li
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China
Qian-Ru Li
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China
Lei-Lei Gao*
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China
*
Corresponding author: Lei-Lei Gao; Email: gaoleilei198802@126.com
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Summary

Our previous studies have suggested that spastin, which aggregates on spindle microtubules in oocytes, may promote the assembly of mouse oocyte spindles by cutting microtubules. This action may be related to CRMP5, as knocking down CRMP5 results in reduced spindle microtubule density and maturation defects in oocytes. In this study, we found that, after knocking down CRMP5 in oocytes, spastin distribution shifted from the spindle to the spindle poles and errors in microtubule–kinetochore attachment appeared in oocyte spindles. However, CRMP5 did not interact with the other two microtubule-severing proteins, katanin-like-1 (KATNAL1) and fidgetin-like-1 (FIGNL1), which aggregate at the spindle poles. We speculate that, in oocytes, due to the reduction of spastin distribution on chromosomes after knocking down CRMP5, microtubule–kinetochore errors cannot be corrected through severing, resulting in meiotic division abnormalities and maturation defects in oocytes. This finding provides new insights into the regulatory mechanisms of spastin in oocytes and important opportunities for the study of meiotic division mechanisms.

Type
Research Article
Information
Zygote , Volume 32 , Issue 1 , February 2024 , pp. 21 - 27
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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