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Construction of Ipr1 expression vector and development of cloned embryos in vitro

Published online by Cambridge University Press:  26 August 2011

Yongli Song ,
Xiaoning He ,
Song Hua ,
Jie Lan ,
Yonggang Liu ,
Pang Cheng ,
Hailin Zhang ,
Jixia Li ,
Xiaoying He  and
Jun Liu
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Yongli Song*
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Xiaoning He
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Song Hua
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Jie Lan
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Yonggang Liu
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Pang Cheng
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Hailin Zhang
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Jixia Li
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Xiaoying He
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Jun Liu
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Yong Zhang
Affiliation:
Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
*
All correspondence to: Yongli Song. Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China. Tel: +86 29 87080092. Fax: +86 29 87080092. E-mail address: Zhy1956p@163.com
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Summary

The purpose of this study was to prepare intracellular pathogen resistance 1 (Ipr1) transgenic donor cells for somatic cell nuclear transfer (SCNT). Based on our current understanding of Ipr1, a macrophage special expression vector pSP–EGFP–Ipr1was constructed. Bovine fetal fibroblasts were transfected with pSP-EGFP-Ipr1. The green fluorescent protein (GFP)-expressing cells were selected and transferred into enucleated bovine oocytes. Then, the rates of oocyte cleavage and blastocyst formation of transgenic cells and non-transgenic cells were observed, respectively. The results showed that reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive. This study may provide cloned embryos for the production of anti-tuberculosis transgenic animals.

Keywords

BovineCloned embryosDonor cellsIpr1Tuberculosis
Type
Research Article
Information
Zygote , Volume 21 , Issue 3 , August 2013 , pp. 265 - 269
Copyright
Copyright © Cambridge University Press 2011 

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