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Construction of Ipr1 expression vector and development of cloned embryos in vitro

  • Yongli Song (a1), Xiaoning He (a1), Song Hua (a1), Jie Lan (a1), Yonggang Liu (a1), Pang Cheng (a1), Hailin Zhang (a1), Jixia Li (a1), Xiaoying He (a1), Jun Liu (a1) and Yong Zhang (a2)...

Summary

The purpose of this study was to prepare intracellular pathogen resistance 1 (Ipr1) transgenic donor cells for somatic cell nuclear transfer (SCNT). Based on our current understanding of Ipr1, a macrophage special expression vector pSP–EGFP–Ipr1was constructed. Bovine fetal fibroblasts were transfected with pSP-EGFP-Ipr1. The green fluorescent protein (GFP)-expressing cells were selected and transferred into enucleated bovine oocytes. Then, the rates of oocyte cleavage and blastocyst formation of transgenic cells and non-transgenic cells were observed, respectively. The results showed that reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive. This study may provide cloned embryos for the production of anti-tuberculosis transgenic animals.

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Corresponding author

All correspondence to: Yongli Song. Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China. Tel: +86 29 87080092. Fax: +86 29 87080092. E-mail address: Zhy1956p@163.com

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