Hostname: page-component-5c6d5d7d68-txr5j Total loading time: 0 Render date: 2024-08-20T11:16:54.821Z Has data issue: false hasContentIssue false
  • English
  • Français

ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies

Published online by Cambridge University Press:  01 November 2006

Masashi Nagano ,
Seiji Katagiri  and
Yoshiyuki Takahashi
Masashi Nagano
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Seiji Katagiri
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
Yoshiyuki Takahashi*
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
*
All correspondence to: Y. Takahashi, Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku Kita 18 Nishi 9, Sapporo 060-0818, Japan. Tel: +81 11 706 5231. Fax: +81 11 706 5232. e-mail: ytaka@vetmed.hokudai.ac.jp
Get access Rights & Permissions [Opens in a new window]

Summary

We examined the relationship among morphological appearance (six groups) of bovine oocytes, ATP content and maturational/developmental ability. Oocytes with a brown ooplasm (with or without a dark region) had intermediate levels of ATP at the germinal vesicle (GV) stage and showed higher rates of first polar body (PB) extrusion than the other groups. Oocytes with a low level of ATP (oocytes with a pale ooplasm without dark clusters) and oocytes with a high level of ATP (oocytes with a black ooplasm) showed lower rates of PB extrusion. During in vitro maturation, ATP levels in oocytes decreased at around GV breakdown and increased toward metaphase II (MII). MII oocytes having a brown ooplasm with a dark region, which had good developmental capacity, had a relatively high level of ATP. MII oocytes with a brown or pale ooplasm without dark clusters, which had poor developmental capacity, had low ATP levels. MII oocytes with a black ooplasm, which had poor developmental capacity, had an unusually high level of ATP. These results suggest that the morphological appearance of bovine oocytes is closely related to their ATP levels and that cytoplasmic morphology will give an advantage for the selection of oocytes with a high maturational and developmental ability.

Keywords

ATPDevelopmental abilityMaturationMitochondriaOocyte morphology
Type
Research Article
Information
Zygote , Volume 14 , Issue 4 , November 2006 , pp. 299 - 304
Copyright
Copyright © Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barnett, D.K., Kimura, J. & Bavister, B.D. (1996). Translocation of active mitochondria during hamster preimplantation embryo development studies by confocal laser scanning microscopy. Dev. Dyn. 205, 64–72.3.0.CO;2-3>CrossRefGoogle ScholarPubMed
Bavister, B.D., Leibfried, M.L. & Lieberman, G. (1983). Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol. Reprod. 28, 235–47.Google Scholar
Calarco, P.G. (1995). Polarization of mitochondria in the unfertilized mouse oocyte. Mol. Reprod. Dev. 16, 36–43.Google ScholarPubMed
Cetica, P., Pintos, L., Dalvit, G. & Beconi, M. (2002). Activity of key enzymes involved in glucose and triglyceride catabolism during bovine oocyte maturation in vitro. Reproduction 124, 675–81.CrossRefGoogle ScholarPubMed
Coenen, K., Massicotte, L. & Sirard, M.A. (2004). Study of newly synthesized proteins during bovine oocytes maturation in vitro using image analysis of two-dimensional gel electrophoresis. Mol. Reprod. Dev. 67, 313–22.Google Scholar
Hyttel, P., Xu, K.P., Smith, S. & Greve, T. (1986). Ultrastructure of in vitro oocyte maturation in cattle. J. Reprod. Fertil. 78, 615–25.CrossRefGoogle ScholarPubMed
Kim, J.Y., Kinoshita, M., Ohnishi, M. & Fukui, Y. (2001). Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes. Reproduction 122, 131–8.CrossRefGoogle ScholarPubMed
Kruip, T.A.M., Cran, D.G., Van Beneden, T.H. & Dieleman, S.J. (1983). Structural changes in bovine oocytes during final maturation in vivo. Gamete Res. 8, 29–47.Google Scholar
Nagano, M., Takahashi, Y. & Katagiri, S. (1999). In vitro fertilization and cortical granule distribution of bovine oocytes having heterogeneous ooplasm with dark clusters. J. Vet. Med. Sci. 61, 531–5.CrossRefGoogle ScholarPubMed
Nagano, M., Katagiri, S. & Takahashi, Y. (2006). Relationship between bovine oocyte morphology and in vitro developmental potential. Zygote 14, 53–61.CrossRefGoogle ScholarPubMed
Rieger, D. (1997). Batch analysis of the ATP content of bovine sperm, oocytes, and early embryos using a scintillation counter to measure the chemiluminescence produced by the luciferin-luciferase reaction. Anal. Biochem. 246, 67–70.CrossRefGoogle ScholarPubMed
Rieger, D. & Loskutoff, N. (1994). Changes in the metabolism of glucose, pyruvate, glutamine and glycine during maturation of cattle oocytes. J. Reprod. Fertil. 100, 257–62.Google Scholar
Sirard, M.A., Florman, H.M., Leibfried-Rutledge, M.L., Barnes, F.L., Sims, M.L. & First, N.L. (1989). Timing of nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes. Biol. Reprod. 40, 1257–63.CrossRefGoogle ScholarPubMed
Stojkovic, M., Machado, S.A., Stojkovic, P., Zakhartchenko, V., Hutzler, P., Goncalves, P.B. & Wolf, E. (2001). Mitochondrial distribution and adenosine triphosphate content of bovine oocytes before and after in vitro maturation: correlation with morphological criteria and developmental capacity after in vitro fertilization and culture. Biol. Reprod. 64, 904–9.Google Scholar
Takahashi, Y., Hishinuma, M., Matsui, M., Tanaka, H. & Kanagawa, H. (1996). Development of in vitro matured/fertilized bovine embryos in a chemically defined medium: influence of oxygen concentration in the gas atmosphere. J. Vet. Med. Sci. 58, 897–902.Google Scholar
Van Blerkom, J. & Runner, M.N. (1984). Mitochondrial reorganization during resumption of arrested meiosis in the mouse oocyte. Am. J. Anat. 171, 335–55.CrossRefGoogle ScholarPubMed
Van Blerkom, J., Davis, P.W. & Lee, J. (1995). ATP content of human oocytes and developmental potential and outcome after in vitro fertilization and embryo transfer. Hum. Reprod. 10, 415–24.Google Scholar
Wanka, F. & Van Zoelen, E.J.J. (2003). Force generation by cellular motors. Cell. Mol. Biol. Lett. 8, 1017–33.Google ScholarPubMed