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Assessment of cGMP level in medium during in vitro growth period of murine preantral follicles with and without supplementation of C-type natriuretic peptide

Published online by Cambridge University Press:  22 June 2021

Li Ang ,
Guo Xingping ,
Cao Haixia ,
Wang Zhulin  and
Wang Huaixiu Open the ORCID record for Wang Huaixiu [Opens in a new window]
Li Ang
Affiliation:
Department of Biochemistry, Shanxi Medical University, Taiyuan, 030001, China
Guo Xingping
Affiliation:
Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China
Cao Haixia
Affiliation:
Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China
Wang Zhulin
Affiliation:
School of Environment and Engineering, Tianjin Industrial University, Tianjin, 300387, China
Wang Huaixiu*
Affiliation:
Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China Beijing Perfect Family Hospital, Beijing, 100034, China
*
Author for correspondence: Wang Huaixiu. Shanxi Provincial Key Laboratory of Cell Regeneration and Birth Defects, Taiyuan, 030001, China. E-mail: 976378008@qq.com
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Summary

To enhance the developmental competency of murine ovarian follicles cultured in vitro, C-type natriuretic peptide (CNP) was supplemented in the culture system. Although the mechanism is not fully elucidated, it was reported that the effect of CNP supplementation was mediated by increased cyclic guanosine monophosphate (cGMP). In the present study, cGMP levels in media for murine preantral follicle culture were compared both between a control group without CNP supplementation and an experimental group with CNP supplementation and between days in each group. In addition, follicle growth patterns and oocyte maturity were assessed and compared between the two groups. Results demonstrated that along with in vitro culture, cGMP levels increased (P < 0.05) both in the control group and the experimental group, whereas cGMP levels were not significantly different between the two groups on the same day of in vitro culture (P > 0.05). The oocyte’s maturity was superior in the experimental group compared with the control group (P < 0.05). As ovarian follicles grew three-dimensionally in the experimental group but were flattened in the control group, CNP might improve oocyte maturity through maintaining the three-dimensional architecture of the ovarian follicle because of increased transzonal projections (TZP) and functional gap junctions between oocyte and surrounding granulosa cells.

Keywords

C-type natriuretic peptideCyclic guanosine monophosphateIn vitro fertilizationIn vitro growthMiceOocyteOvarian follicle culture
Type
Research Article
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 98 - 102
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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