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Assessment of binder of sperm protein 1 (BSP1) and heparin effects on in vitro capacitation and fertilization of bovine ejaculated and epididymal sperm

Published online by Cambridge University Press:  10 August 2020

Paula Rodríguez-Villamil ,
Daiane Mentz ,
Felipe Ledur Ongaratto Open the ORCID record for Felipe Ledur Ongaratto [Opens in a new window] ,
Luis Henrique Aguiar ,
Jose Luiz Rodrigues ,
Marcelo Bertolini  and
Arlindo A. Moura Open the ORCID record for Arlindo A. Moura [Opens in a new window]
Paula Rodríguez-Villamil
Affiliation:
Animal Physiology Laboratory, Federal University of Ceará, Fortaleza, Brazil Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Daiane Mentz
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Felipe Ledur Ongaratto
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Luis Henrique Aguiar
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Jose Luiz Rodrigues
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Marcelo Bertolini
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Arlindo A. Moura*
Affiliation:
Animal Physiology Laboratory, Federal University of Ceará, Fortaleza, Brazil
*
Author for correspondence: Arlindo A. Moura. Department of Animal Science. Av. Mister Hull, s/n Campus do Pici. FortalezaCE60440-554, Brazil. Tel: +55 85 3366 9697. E-mail: arlindo.moura@gmail.com
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Summary

The present study evaluated the effect of binder of sperm protein 1 (BSP1) and/or heparin on in vitro bovine capacitation and fertilization rates using epididymal and ejaculated bovine sperm. Frozen–thawed sperm were selected and used in the following treatments. Control group: Fert-TALP medium without heparin; heparin (HEP) group: Fert-TALP with heparin (10 UI/ml); BSP1 group: Fert-TALP medium with BSP1 (10 µg/ml for ejaculated sperm; 40 µg/ml for epididymal sperm); HEP + BSP1 group: Fert-TALP medium with heparin (5 UI/ml) and BSP1 (5 µg/ml for ejaculated sperm; 20 µg/ml for epididymal sperm) and determined in vitro capacitation rates in different interval times (0, 15, 30 and 60 min) using the chlortetracycline fluorescence (CTC) method. Also, we evaluated the development rates of oocytes fertilized with ejaculated or epididymal sperm into the same treatments. Capacitation was greater and faster when ejaculated sperm were treated for 60 min with heparin compared with other treatments. However, developmental rates were similar in all treatments. For epididymal sperm, the treatments with BSP1 presented higher capacitation and fertilization rates compared with heparin (P < 0.05). The effects of heparin + BSP1 on capacitation and developmental rates did not cause any increase in capacitation or blastocyst rates compared with other groups for ejaculated or epididymal sperm. In conclusion, this study confirmed that either BSP1 and heparin can be used as capacitator agents for bovine ejaculated sperm during IVF. However, BSP1 seems to be more efficient compared with heparin for epididymal sperm. Furthermore, BSP1 and heparin have no synergic effects on sperm capacitation.

Keywords

BSP1HeparinIVFSperm capacitationSperm protein
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 489 - 494
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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