Skip to main content Accessibility help
×
Home

Adaptation of a trap door technique for the recovery of ovarian cortical biopsies from Cebus apella (capuchin monkey)

  • Luana N. Santana (a1) (a2), Adriel B. Brito (a1) (a2), Danielle C. Brito (a1), Julianne S. Lima (a1) (a2), Sheyla F.S. Domingues (a1) (a2) and Regiane R. Santos (a3) (a1) (a2)...

Summary

There is a paucity of efficient cryopreservation protocols for primordial follicles enclosed in the ovarian tissue from non-human primates (NHP), in special New World primates. Our objective was to establish an optimal procedure for the recovery of ovarian biopsies from capuchin monkeys. To this end, we adapted a trap door biopsy method. Follicular density and quality of the biopsies were evaluated and ultrasound analysis was performed before and continuously after surgery to assess ovarian structure. Ovarian tissue biopsies recovered by the trap door technique allowed the successful harvesting of primordial follicles from capuchin monkeys, and no complication was recorded. The female cycle was not affected by surgery and no adherence was found thereafter. In conclusion, the adaptation of a trap door biopsy method is a safe procedure and allows recovery of healthy primordial follicles.

Copyright

Corresponding author

All correspondence to: Regiane R. Santos. Faculty of Veterinary Medicine, Yalelaan 104, 3584 CM, Utrecht, The Netherlands. Tel: +31 30 2531078. Fax: +31 30 2534125. e-mail: R.Rodriguesdossantos@pq.cnpq.br

References

Hide All
Domingues, S.F.S., Diniz, L.V., Furtado, S.H.C., Ohashi, O.M., Rondina, D. & Silva, L.D.M. (2004). Histological study of capuchin monkey (Cebus apella) ovarian follicles. Acta Amazonica 30, 495501.
Domingues, S.F.S., Caldas-Bussiere, M.C., Martins, N.D. & Carvalho, R.A. (2007). Ultrasonographic imaging of the reproductive tract and surgical recovery of oocytes in Cebus apella (capuchin monkeys). Theriogenology 68, 1251–9.
Domingues, S.F.S., Caldas-Bussiere, M.C., Petretski, M.D., Ohashi, O.M., Lima, J.S., Santos, R.R., Cordeiro, M.S. & Gomes de Castro, P.H. (2010). Effects of follicular phase and oocyte-cumulus complexes quality on the protein profile and in vitro oocyte meiosis competence in Cebus apella. Fertil. Steril. 93, 1662–7.
Donnez, J., Silber, S., Andersen, C.Y., Demeestere, I., Piver, P., Merow, D., Pellicer, A. & Dolmans, M.M. (2011). Children born after autotransplantation of cryopreserved ovarian tissue. A review of 13 live births. Ann. Med. 43, 437–50.
Edel, A. (1974). Clinical evaluation of free connective tissue grafts used to increase the width of keratinised gingiva. J. Clin. Periodontol. 1, 185–96.
Figueiredo, J.R., Rodrigues, A.P., Silva, J.R. & Santos, R.R. (2011). Cryopreservation and in vitro culture of caprine preantral follicles. Reprod. Fertil. Dev. 23, 40–7.
Meirow, D., Fasouliotis, S.J., Nugent, D., Schenker, J.G., Gosden, R.G. & Rutherford, A.J. (1999). A laparoscopic technique for obtaining ovarian cortical biopsy specimens for fertility conservation in patients with cancer. Fertil. Steril. 71, 948–51.
Santos, R.R., Rodrigues, A.P., Costa, S.H., Silva, J.R., Matos, M.H., Lucci, C.M., Báo, S.N., Van den Hurk, R. & Figueiredo, J.R. (2006). Histological and ultrastructural analysis of cryopreserved sheep preantral follicles. Anim. Reprod. Sci. 91, 249–63.
Santos, R.R., Amorim, C., Cecconi, S., Fassbender, M., Imhof, M., Lornage, J., Paris, M., Schoenfeldt, V. & Martinez-Madrid, B. (2010). Cryopreservation of ovarian tissue: an emerging technology for female germline preservation of endangered species and breeds. Anim. Reprod. Sci. 122, 151–63.
Taskin, U., Yigit, O., Bilici, S., Kuvat, S.V., Sisman, A.S. & Celebi, S. (2011). Efficacy of the combination of intraoperative cold saline-soaked gauze compression and corticosteroids on rhinoplasty morbidity. Otolaryngol. Head Neck Surg. [Epub ahead of print] DOI: 10.1177/0194599811400377.

Keywords

Adaptation of a trap door technique for the recovery of ovarian cortical biopsies from Cebus apella (capuchin monkey)

  • Luana N. Santana (a1) (a2), Adriel B. Brito (a1) (a2), Danielle C. Brito (a1), Julianne S. Lima (a1) (a2), Sheyla F.S. Domingues (a1) (a2) and Regiane R. Santos (a3) (a1) (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed