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Accelerated modification of the zona pellucida is the primary cause of decreased fertilizability of oocytes in the 129 inbred mouse strain

Published online by Cambridge University Press:  21 December 2010

Toshiaki Hino ,
Kanako Oda ,
Kenji Nakamura ,
Hiroyuki Tateno ,
Yutaka Toyoda  and
Minesuke Yokoyama
Toshiaki Hino*
Affiliation:
Department of Biological Sciences, Asahikawa Medical University, 2–1 Midorigaoka-higashi, Asahikawa 078–8510, Japan. Mitsubishi Kagaku Institute of Life Sciences (MITILS), Tokyo, Japan.
Kanako Oda
Affiliation:
Mitsubishi Kagaku Institute of Life Sciences (MITILS), Tokyo, Japan. Center for Bioresource-based Researches, Brain Research Institute, Niigata University, Niigata, Japan.
Kenji Nakamura
Affiliation:
Mitsubishi Kagaku Institute of Life Sciences (MITILS), Tokyo, Japan.
Hiroyuki Tateno
Affiliation:
Department of Biological Sciences, Asahikawa Medical University, Hokkaido, Japan.
Yutaka Toyoda
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Minesuke Yokoyama
Affiliation:
Mitsubishi Kagaku Institute of Life Sciences (MITILS), Tokyo, Japan. Center for Bioresource-based Researches, Brain Research Institute, Niigata University, Niigata, Japan.
*
All correspondence to: Toshiaki Hino, Department of Biological Sciences, Asahikawa Medical University, 2–1 Midorigaoka-higashi, Asahikawa 078–8510, Japan. Tel: +81 166 68 2731. Fax: +81 166 68 2783. e-mail: hino@asahikawa-med.ac.jp
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Summary

We investigated whether the small litter size in the 129 inbred mouse strain results from a reduction in oocyte fertilizability. Sensitivity of the zona pellucida to α-chymotrypsin was examined for oocytes collected at 14 h (shortly after ovulation), 17 h, and 20 h after hCG injection. Passage of spermatozoa through the zona pellucida (using an in vitro fertilization (IVF) technique) and the density of cortical granules were examined for oocytes collected at 14 and 17 h after hCG injection. The capability of the oolemma to fuse with the sperm plasma membrane was also evaluated by IVF using zona-free eggs. The zona pellucida became markedly resistant to the enzyme 17 h after hCG injection. IVF rates significantly decreased at this time. In addition, there was a significant reduction in the density of cortical granules. When zona-free oocytes were inseminated, high fertilization rates were obtained at both 17 and 14 h after hCG injection. These results indicate that accelerated modification of the zona pellucida primarily causes a decreased fertilizability of oocytes in 129 mice, resulting in the low reproductive performance of this strain.

Keywords

129 mouseCortical granulesFertilityOocyteZona pellucida
Type
Research Article
Information
Zygote , Volume 19 , Issue 4 , November 2011 , pp. 315 - 322
Copyright
Copyright © Cambridge University Press 2010

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