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Accelerated modification of the zona pellucida is the primary cause of decreased fertilizability of oocytes in the 129 inbred mouse strain

  • Toshiaki Hino (a1) (a2), Kanako Oda (a2) (a3), Kenji Nakamura (a2), Hiroyuki Tateno (a4), Yutaka Toyoda (a5) and Minesuke Yokoyama (a2) (a3)...

Summary

We investigated whether the small litter size in the 129 inbred mouse strain results from a reduction in oocyte fertilizability. Sensitivity of the zona pellucida to α-chymotrypsin was examined for oocytes collected at 14 h (shortly after ovulation), 17 h, and 20 h after hCG injection. Passage of spermatozoa through the zona pellucida (using an in vitro fertilization (IVF) technique) and the density of cortical granules were examined for oocytes collected at 14 and 17 h after hCG injection. The capability of the oolemma to fuse with the sperm plasma membrane was also evaluated by IVF using zona-free eggs. The zona pellucida became markedly resistant to the enzyme 17 h after hCG injection. IVF rates significantly decreased at this time. In addition, there was a significant reduction in the density of cortical granules. When zona-free oocytes were inseminated, high fertilization rates were obtained at both 17 and 14 h after hCG injection. These results indicate that accelerated modification of the zona pellucida primarily causes a decreased fertilizability of oocytes in 129 mice, resulting in the low reproductive performance of this strain.

Copyright

Corresponding author

All correspondence to: Toshiaki Hino, Department of Biological Sciences, Asahikawa Medical University, 2–1 Midorigaoka-higashi, Asahikawa 078–8510, Japan. Tel: +81 166 68 2731. Fax: +81 166 68 2783. e-mail: hino@asahikawa-med.ac.jp

References

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Barlow, P., Englert, Y., Puissant, F., Lejeune, B., Delvigne, A., Van Rysselberge, M. & Leroy, F. (1990). Fertilization failure in IVF: why and what next? Hum. Reprod. 5, 451–6.
Barros, C. & Yanagimachi, R. (1971). Induction of zona reaction in golden hamster eggs by cortical granule material. Nature 233, 268–9.
Braden, A.W.H. (1962). Spermatozoon penetration and fertilization in the mouse. Symp. Genet. Biol. Italica Pavia Italy 9, 94101.
Braden, A.W.H. & Austin, C.R. (1954). Fertilization of the mouse egg and the effect of delayed coitus and of hot-shock treatment. Aust. J. Biol. Sci. 7, 552–65.
Buhi, W.C. (2002). Characterization and biological roles of oviduct-specific, oestrogen-dependent glycoprotein. Reproduction 123, 355–62.
Byers, S.L., Payson, S.J. & Taft, R.A. (2006). Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs). Theriogenology 65, 1716–26.
Collas, P., Balise, J.J., Hofmann, G.A. & Robl, J.M. (1989). Electrical activation of mouse oocytes. Theriogenology 32, 835–44.
Coy, P., Cánovas, S., Mondéjar, I., Saavedra, M.D., Romar, R., Grullón, L., Matás, C. & Avilés, M. (2008). Oviduct-specific glycoprotein and heparin modulate sperm-zona pellucida interaction during fertilization and contribute to the control of polyspermy. Proc. Natl. Acad. Sci. USA 105, 15809–14.
Ducibella, T., Anderson, E., Albertini, D.F., Aalberg, J. & Rangarajan, S. (1988). Quantitative studies of changes in cortical granule number and distribution in the mouse oocyte during meiotic maturation. Dev. Biol. 130, 184–97.
Edwards, R.G. & Gates, A.H. (1959). Timing of the stages of the maturation divisions, ovulation, fertilization and the first cleavage of eggs of adult mice treated with gonadotrophins. J. Endocrinol. 18, 292304.
Evans, M.J. & Kaufman, M.H. (1981). Establishment in culture of pluripotential cells from mouse embryos. Nature 292, 154–6.
Festing, M.F.W. (1979). Inbred Strains in Biomedical Research. pp. 137266. New York: Oxford University Press.
Fulton, B.P. & Whittingham, D.G. (1978). Activation of mammalian oocytes by intracellular injection of calcium. Nature 273, 149–51.
Gulyas, B.J. & Yuan, L.C. (1985). Cortical reaction and zona hardening in mouse oocytes following exposure to ethanol. J. Exp. Zool. 233, 269–76.
Hino, T., Oda, K., Nakamura, K., Toyoda, Y. & Yokoyama, M. (2009). Low fertility in vivo resulting from female factors causes small litter size in 129 inbred mice. Reprod. Med. Biol. 8, 157–61.
Kawai, Y., Hata, T., Suzuki, O. & Matsuda, J. (2006). The relationship between sperm morphology and in vitro fertilization ability in mice. J. Reprod. Dev. 52, 561–8.
Krzanowska, H. (1972). Rapidity of removal in vitro of the cumulus oophorus and the zona pellucida in different strains of mice. J. Reprod. Fert. 31, 714.
Kubiak, J.Z. (1989). Mouse oocytes gradually develop the capacity for activation during the metaphase II arrest. Dev. Biol. 136, 537–45.
Lyng, R. & Shur, B.D. (2009). Mouse oviduct-specific glycoprotein is an egg-associated ZP3-independent sperm-adhesion ligand. J. Cell Sci. 122, 3894–906.
Männikkö, M., Törmälä, R.M., Tuuri, T., Haltia, A., Martikainen, H., Ala-Kokko, L., Tapanainen, J.S. & Lakkakorpi, J.T. (2005). Association between sequence variations in genes encoding human zona pellucida glycoproteins and fertilization failure in IVF. Hum. Reprod. 20, 1578–85.
Martin, G.R. (1981). Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma. Proc. Natl. Acad. Sci. USA. 78, 7634–8.
Matin, A. (2007). What leads from dead-end? Cell. Mol. Life Sci. 64, 1317–22.
Mintz, B. & Gearhart, J.D. (1973). Subnormal zona pellucida change in parthenogenetic mouse embryos. Dev. Biol. 31, 178–84.
Molloy, D., Harrison, K., Breen, T. & Hennessey, J. (1991). The predictive value of idiopathic failure to fertilization on the first in vitro fertilization attempt. Fertil. Steril. 56, 285–9.
Nagasawa, H., Miyamoto, M. & Fujimoto, M. (1973). Reproductivity in inbred strains of mice and project for their efficient production. Jikken Dobutsu 22, 119–26. [In Japanese]
Nomura, T. & Katsuki, M. (eds.) (1987). Hassei Kougaku Jikken Manual, p. 15. Tokyo: Kodansha-Scientific. [In Japanese]
Olds-Clarke, P. (1996). How does poor motility alter sperm fertilizing ability? J. Androl. 17, 183–6.
Roest, J., Van Heusden, A.M., Zeilmaker, G.H. & Verhoeff, A. (1998). Treatment policy after poor fertilization in the first IVF cycle. J. Assist. Reprod. Genet. 15, 1821.
Sato, K. (1979). Polyspermy – preventing mechanisms in mouse eggs fertilized in vitro. J. Exp. Zool. 210, 353–59.
Schmell, E.D. & Gulyas, B.J. (1980). Ovoperoxidase activity in ionophore treated mouse eggs. II. Evidence for the enzyme's role in hardening the zona pellucida. Gamete Res. 3, 279–90.
Smithberg, M. (1953). The effect of different proteolytic enzymes on the zona pellucida of mouse ova. Anat. Rec. 117, 554.
Stevens, L.C. (1967). The biology of teratomas. Adv. Morphog. 6, 131.
Suzuki-Migishima, R., Hino, T., Takabe, M., Oda, K., Migishima, F., Morimoto, Y., & Yokoyama, M. (2009). Marked improvement of fertility of cryopreserved C57BL/6J mouse sperm by depletion of Ca2+ in medium. J. Reprod. Dev. 55, 386–92.
Sztein, J.M., Farley, J.S. & Mobraaten, L.E. (2000). In vitro fertilization with cryopreserved inbred mouse sperm. Biol. Reprod. 63, 1774–80.
Toyoda, Y., Yokoyama, M. & Hosi, T. (1971a). Studies on the fertilization of mouse eggs in vitro. I. In vitro fertilization of eggs by fresh epididymal sperm. Jpn. J. Anim. Reprod. 16, 147–51.
Toyoda, Y., Yokoyama, M. & Hosi, T. (1971b). Studies on the fertilization of mouse eggs in vitro. II. Effects of in vitro preincubation of spermatozoa on time of sperm penetration of mouse eggs in vitro. Jpn. J. Anim. Reprod. 16, 152–7.
Verley, F.A., Grahn, D., Leslie, W.P. & Hamilton, K.F. (1967). Sex ration of mice as possible indicator of mutation rate for sex-linked lethals. J. Hered. 58, 285–90.
Xu, Z., Abbott, A., Kopf, G.S., Schultz, R.M. & Ducibella, T. (1997). Spontaneous activation of ovulated mouse eggs: time-dependent effects on M-phase exit, cortical granule exocytosis, maternal messenger ribonucleic acid recruitment, and inositol 1,4,5-trisphosphate sensitivity. Biol. Reprod. 57, 743–50.

Keywords

Accelerated modification of the zona pellucida is the primary cause of decreased fertilizability of oocytes in the 129 inbred mouse strain

  • Toshiaki Hino (a1) (a2), Kanako Oda (a2) (a3), Kenji Nakamura (a2), Hiroyuki Tateno (a4), Yutaka Toyoda (a5) and Minesuke Yokoyama (a2) (a3)...

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