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Safety evaluation of single-sperm cryopreservation technique applied in intracytoplasmic sperm injection

Published online by Cambridge University Press:  17 April 2024

Duanjun Zhang
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Wenliang Yao
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Mingliang Zhang
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Lijuan Yang
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Lin Li
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Shujuan Liu
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Xianglong Jiang
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Yingli Sun
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Shuonan Hu
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Yufang Huang
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Jie Xue
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Xiaoting Zheng
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Qi Xiong
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Shenghui Chen*
Affiliation:
Department of Reproductive Medicine, Nanchang Xinhua Hospital, Nanchang Reproductive Hospital, Reproductive Hospital Affiliated to Jiangxi University of Chinese Medicine, Nanchang City, 330001, Jiangxi Province, China
Haiqin Zhu*
Affiliation:
Department of Pediatrics, The Second Affiliated Hospital of Nanchang University, Nanchang City, 330000, Jiangxi Province, China
*
Corresponding author: Haiqin Zhu; Email: Haiqinzhu1@163.com and Shenghui Chen; Email: np0287741yue@163.com
Corresponding author: Haiqin Zhu; Email: Haiqinzhu1@163.com and Shenghui Chen; Email: np0287741yue@163.com

Abstract

Intracytoplasmic sperm injection (ICSI) is a technique that directly injects a single sperm into the cytoplasm of mature oocytes. Here, we explored the safety of single-sperm cryopreservation applied in ICSI. This retrospective study enrolled 186 couples undergoing ICSI-assisted pregnancy. Subjects were allocated to the fresh sperm (group A)/single-sperm cryopreservation (group B) groups based on sperm type, with their clinical baseline/pathological data documented. We used ICSI-compliant sperm for subsequent in vitro fertilization and followed up on all subjects. The recovery rate/cryosurvival rate/sperm motility of both groups, the pregnancy/outcome of women receiving embryo transfer, and the delivery mode/neonatal-related information of women with successful deliveries were recorded. The clinical pregnancy rate, cumulative clinical pregnancy rate, abortion rate, ectopic pregnancy rate, premature delivery rate, live birth delivery rate, neonatal birth defect rate, and average birth weight were analyzed. The two groups showed no significant differences in age, body mass index, ovulation induction regimen, sex hormone [anti-Müllerian hormone (AMH)/follicle-stimulating hormone (FSH)/luteinizing hormone (LH)] levels, or oocyte retrieval cycles. The sperm recovery rate (51.72%-100.00%) and resuscitation rate (62.09% ± 16.67%) in group B were higher; the sperm motility in the two groups demonstrated no significant difference and met the ICSI requirements. Group B exhibited an increased fertilization rate, decreased abortion rate, and increased safety versus group A. Compared with fresh sperm, the application of single-sperm cryopreservation in ICSI sensibly improved the fertilization rate and reduced the abortion rate, showing higher safety.

Type
Research Article
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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Footnotes

*

Duanjun Zhang and Wenliang Yao contributed equally to this work.

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