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Replacement of glutamine with the dipeptide derivative alanyl-glutamine enhances in vitro maturation of porcine oocytes and development of embryos

Published online by Cambridge University Press:  27 February 2013

Su Jin Kim ,
Ok Jae Koo ,
Dae Kee Kwon ,
Jung Taek Kang ,
Sol Ji Park ,
Ma Ninia Gomez ,
Mohammad Atikuzzaman ,
Goo Jang  and
Byeong-Chun Lee
Su Jin Kim
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Ok Jae Koo
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Dae Kee Kwon
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Jung Taek Kang
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Sol Ji Park
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Ma Ninia Gomez
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Mohammad Atikuzzaman
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Goo Jang
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
Byeong-Chun Lee*
Affiliation:
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea.
*
All correspondence to Byeong-Chun Lee. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 599 Gwanangno, Gwanak-gu, Seoul 151–742, Korea. Tel: +82 2 880 1269. Fax: +82 2 873 1269. E-mail: bclee@snu.ac.kr
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Summary

The presence of glutamine (Gln) in in vitro maturation (IVM) and in vitro culture (IVC) medium is a more potent factor for improving porcine oocyte and embryo development than other amino acids. However Gln is inherently unstable and spontaneously breaks down into ammonia, and therefore interferes with proper development. To avoid this adverse effect, Gln was replaced in the present study with its stable dipeptide derivative alanyl-glutamine (Ala-Gln) and the effects of this replacement on porcine IVM and IVC were evaluated. Replacement of Gln with Ala-Gln during IVM did not improve nuclear maturation, however numbers of early cleaved embryos were significantly increased after activation. Blastocyst formation rates were also significantly improved by using Ala-Gln during IVM. Replacement of Gln with Ala-Gln during IVC significantly increased total cell numbers in blastocysts. Blastocyst formation rate was also significantly higher when Ala-Gln was used in both IVM and IVC. In conclusion, the use of Ala-Gln rather than Gln gives better results for development in both porcine IVM and IVC.

Keywords

Alanyl-glutamineGlutaMAXGlutamineIn vitro maturationPorcine embryo culture
Type
Short Communications
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 286 - 289
Copyright
Copyright © Cambridge University Press 2013 

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