Hostname: page-component-848d4c4894-xfwgj Total loading time: 0 Render date: 2024-06-14T09:24:06.289Z Has data issue: false hasContentIssue false

Number of blastomeres and distribution of microvilli in cloned mouse embryos during compaction

Published online by Cambridge University Press:  25 August 2010

Chao-Bo Li
Affiliation:
Department of Histology and Embryology, Harbin Medical University, Harbin, China.
Zhen-Dong Wang
Affiliation:
Department of Histology and Embryology, Harbin Medical University, Harbin, China.
Zhong Zheng
Affiliation:
Department of Histology and Embryology, Harbin Medical University, Harbin, China.
Li-Li Hu
Affiliation:
Department of Histology and Embryology, Harbin Medical University, Harbin, China.
Shu-Qi Zhong
Affiliation:
Department of Histology and Embryology, Harbin Medical University, Harbin, China.
Lei Lei*
Affiliation:
Department of Histology and Embryology, Harbin Medical University, 194 Xuefu Road, Nangang District, Harbin, 150081China.
*
All correspondence to: Lei Lei. Department of Histology and Embryology, Harbin Medical University, 194 Xuefu Road, Nangang District, Harbin, 150081China. Tel: +86 451 86674518. Fax: +86 451 87503325. e-mail: leil086@yahoo.com.cn

Summary

The events resulting in compaction have an important influence on the processes related to blastocyst formation. To analyse the quality of the embryos obtained by somatic cell nuclear transfer (SCNT) in aspects different from previous studies, not only the number of blastomeres of cloned embryos during the initiation of compaction, but also the distribution of microvilli in cloned, normal, parthenogenetic, and tetraploid embryos before and after compaction was preliminarily investigated in mouse. Our results showed that during compaction the number of blastomeres in SCNT embryos was fewer than that in intracytoplasmic sperm injection (ICSI) embryos and, before compaction, there was a uniform distribution of microvilli over the blastomere surface, but microvilli became restricted to an apical region after compaction in the four types of embryos. We also reported here that the time course of compaction in SCNT embryos was about 3 h delayed compared with that in ICSI embryos, while there was no significant difference between SCNT and ICSI embryos when developed to the 4-cell stage. We concluded that: (i) the cleavage of blastomeres in cloned embryos was slow at least before compaction; (ii) the distribution of microvilli in cloned, normal, parthenogenetic, and tetraploid embryos was coherent before and after compaction; and (iii) the initiation of compaction in SCNT embryos was delayed compared with that of ICSI embryos.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cui, X.S., Li, X.Y., Shen, X.H., Bae, Y.J., Kang, J.J. & Kim, N.H. (2007). Transcription profile in mouse four-cell, morula, and blastocyst: Genes implicated in compaction and blastocoel formation. Mol. Reprod. Dev. 74, 133–43.CrossRefGoogle ScholarPubMed
Dadi, T.D., Li, M.W. & Lloyd, K.C. (2004). Expression levels of EGF, TGF-alpha, and EGF-R are significantly reduced in pre-implantation cloned mouse embryos. Cloning Stem Cells 6, 267–83.CrossRefGoogle ScholarPubMed
Dadi, T.D., Li, M.W. & Lloyd, K. C. (2006). Development of mouse embryos after immunoneutralization of mitogenic growth factors mimics that of cloned embryos. Comp. Med. 56, 188–95.Google ScholarPubMed
Ducibella, T., Ukena, T., Karnovsky, M. & Anderson, E. (1977). Changes in cell surface and cortical cytoplasmic organization during early embryogenesis in the preimplantation mouse embryo. J. Cell. Biol. 74, 153–67.CrossRefGoogle ScholarPubMed
Kawase, Y., Iwata, T., Toyoda, Y., Wakayama, T., Yanagimachi, R. & Suzuki, H. (2001). Comparison of intracytoplasmic sperm injection for inbred and hybrid mice. Mol. Reprod. Dev. 60, 74–8.CrossRefGoogle ScholarPubMed
Larue, L., Ohsugi, M., Hirchenhain, J. & Kernler, R. (1994). E-cadherin null mutant embryos fail to form a trophectoderm epithelium. Proc. Natl. Acad. Sci. U S A. 91, 8263–7.CrossRefGoogle Scholar
Louvet, S., Aghion, J., Santa-Maria, A., Mangeat, P. & Maro, B. (1996). Ezrin becomes restricted to outer cells following asymmetrical division in the preimplantation mouse embryo. Dev. Biol. 177, 568–79.CrossRefGoogle ScholarPubMed
McLachlin, J.R., Caveney, S. & Kidder, G.M. (1983). Control of gap junction formation in early mouse embryos. Dev. Biol. 98, 155–64.CrossRefGoogle ScholarPubMed
Nelson, W.J. (2008). Regulation of cell–cell adhesion by the cadherin–catenin complex. Biochem. Soc. Trans. 36, 149–55.CrossRefGoogle ScholarPubMed
Pauken, C.M. & Capco, D.G. (1999). Regulation of cell adhesion during embryonic compaction of mammalian embryos: roles for PKC and beta-catenin. Mol. Reprod. Dev. 54, 135–44.3.0.CO;2-A>CrossRefGoogle ScholarPubMed
Pratt, H.P., Ziomek, C.A., Reeve, W.J. & Johnson, M.H. (1982). Compaction of the mouse embryo: an analysis of its components. J. Embryol. Exp. Morphol. 70, 113–32.Google ScholarPubMed
Rybouchkin, A., Heindryckx, B., Van Der Elst, J. & Dhont, M. (2002). Developmental potential of cloned mouse embryos reconstructed by a conventional technique of nuclear injection. Reproduction 124, 197207.CrossRefGoogle ScholarPubMed
Watson, A.J. & Barcroft, L.C. (2001). Regulation of blastocyst formation. Front. Biosci. 6, D70830.CrossRefGoogle ScholarPubMed
Weis, W.I. & Nelson, W.J. (2006). Re-solving the cadherin–catenin–actin conundrum. J. Biol. Chem. 281, 35593–7.CrossRefGoogle ScholarPubMed
Yazawa, H., Yanagida, K. & Sato, A. (2001). Oocyte activation and Ca2+ oscillation-inducing abilities of mouse round/elongated spermatids and the developmental capacities of embryos from spermatid injection. Hum. Reprod. 16, 1221–8.CrossRefGoogle ScholarPubMed