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5-HT-receptive structures are localized in the interblastomere cleft of Paracеntrotus lividus early embryos

  • Yu. B. Shmukler (a1), Francesco Silvestre (a2) and Elisabetta Tosti (a2)

Summary

Local application of the agonists of serotonin receptors of third type 5-HTQ, SR57277A and quipazine into the interblastomere cleft of the sea urchin Paracentrotus lividus embryo during first cleavage division, evokes specific membrane currents, whereas application of these drugs out of contact area show currents of lower amplitude and longer latent period. At the same time 5-HT3-receptor agonist quipazine imitates interblastomere signal in half embryos, but corresponding antagonists prevent it. Present data develop the hypothesis of protosynapse, demonstrating that the distribution of membrane serotonin receptors is limited to the period of cleavage division and localized in the interblastomere contact area. A possible role of spatial–temporal restriction of receptors at the interblastomere contact area is discussed in relation to the subsequent embryo development.

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Corresponding author

1All correspondence to: Elisabetta Tosti. Stazione Zoologica ‘Anton Dohrn’, Villa Comunale 1, 80121, Napoli, Italy. Tel: +39 81 5833288. Fax: +39 81 7641355. e-mail: tosti@szn.it

References

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Buznikov, G.A. (ed.) (1990). Neurotransmitters in Embryogenesis, p. 526. Churchill, Academic Press.
Buznikov, G.A. & Shmukler, Yu.B. (1978). On the influence of antitransmitter substances on cellular interactions in early sea urchin embryos. Sov. J. Dev. Biol. 9, 173–8.
Buznikov, G.A. & Shmukler, Yu.B. (1981). The possible role of ‘prenervous’ neurotransmitters in cellular interactions of early embryogenesis: a hypothesis. Neurochem. Res. 6, 5569.
Shmukler, Yu.B. (1981). Cellular interactions in early sea urchin embryos. III. Influence of neuropharmaca on the cleavage pattern of Scaphechinus mirabilis half. Sov. J. Dev. Biol. 12, 404–9.
Shmukler, Yu.B. (1992). Specific binding of [3H]8-OH-DPAT by early embryos of sea urchin Strongylocentrotus intermedius. Biol. Membr. 9, 1167–9.
Shmukler, Yu.B. (1993). On the possibility of membrane reception of neurotransmitter in sea urchin early embryos. Comp. Biochem. Physiol. 106C, 269–73.
Shmukler, Yu.B. & Buznikov, G.A. (1998). Functional coupling of neurotransmitters with second messengers during cleavage divisions: facts and hypotheses. Perspect. Dev. Neurobiol. 5, 469–80.
Shmukler, Yu.B. & Tosti, E. (2002). Serotonergic-induced ion currents in cleaving sea urchin embryo. Invertebr. Reprod. Dev. 42, 43–9.
Shmukler, Yu.B., Chailakhian, L.M., Smolianinov, V.V., Bliokh, Zh.L., Karpovich, A.L., Gusareva, E.V., Naidenko, T.Kh., Khashaev, Z. Kh-M. & Medvedeva, T.D. (1981). Cellular interactions in early sea urchin embryos. II. Dated mechanical isolation of blastomeres. Sov. J. Dev. Biol. 12, 398403.
Shmukler, Yu.B., Grigoriev, N.G., Buznikov, G.A. & Turpaev, T.M. (1986). Regulation of cleavage divisions: participation of ‘prenervous’ neurotransmitters coupled with second messengers. Comp. Biochem. Physiol. 83C, 423–7.
Vacquier, V.D. & Mazia, D. (1968). Twinning of sand dollar embryos by means of dithiothreitol. The structural basis of blastomere interactions. Exp. Cell Res. 52, 209–19.
Yazaki, I., Tosti, E. & Dale, B. (1995). Cytoskeletal elements link calcium channel activity and the cell cycle in early sea urchin embryos. Development 121, 1827–31.

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