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5-HT-receptive structures are localized in the interblastomere cleft of Paracеntrotus lividus early embryos

Published online by Cambridge University Press:  01 February 2008

Yu. B. Shmukler ,
Francesco Silvestre  and
Elisabetta Tosti
Yu. B. Shmukler
Affiliation:
N.K. Koltzov Institute of Developmental Biology, Russian Acad. Sci. 26, Vavilov st., Moscow, 119334, Russia.
Francesco Silvestre
Affiliation:
Cell Biology Laboratory, Stazione Zoologica, Napoli, Italy.
Elisabetta Tosti*
Affiliation:
Cell Biology Laboratory, Stazione Zoologica, Napoli, Italy.
*
1All correspondence to: Elisabetta Tosti. Stazione Zoologica ‘Anton Dohrn’, Villa Comunale 1, 80121, Napoli, Italy. Tel: +39 81 5833288. Fax: +39 81 7641355. e-mail: tosti@szn.it
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Summary

Local application of the agonists of serotonin receptors of third type 5-HTQ, SR57277A and quipazine into the interblastomere cleft of the sea urchin Paracentrotus lividus embryo during first cleavage division, evokes specific membrane currents, whereas application of these drugs out of contact area show currents of lower amplitude and longer latent period. At the same time 5-HT3-receptor agonist quipazine imitates interblastomere signal in half embryos, but corresponding antagonists prevent it. Present data develop the hypothesis of protosynapse, demonstrating that the distribution of membrane serotonin receptors is limited to the period of cleavage division and localized in the interblastomere contact area. A possible role of spatial–temporal restriction of receptors at the interblastomere contact area is discussed in relation to the subsequent embryo development.

Keywords

Blastomere interactionsCleavage division5-HT receptorsNeurotransmittersProtosynapseSea urchin embryo
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 79 - 86
Copyright
Copyright © Cambridge University Press 2008

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