Field studies at three locations across Nebraska showed that 2,3,6-trichlorobenzoic acid (2,3,6-TBA) leached readily into the soil profile. The herbicide was lost from the surface soil much more readily in central and western Nebraska than in eastern Nebraska even though rainfall was about twice as much at the last location. Volatilization was suggested as a possible reason for this loss of 2,3,6-TBA. There was a definite, but not complete, loss of 2,3,6-TBA from the three soil profiles between May 1960 and September 1961. Soybeans proved to be an acceptable bioassay plant for this herbicide in soil at 2,3,6-TBA concentrations of ½ to 2 ppmw.