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An in vitro Method of Evaluating the Activity of Buds on the Rhizomes of Quackgrass (Agropyron repens)

  • B. G. Johnson (a1) and K. P. Buchholtz (a1)

Extract

One of the major prerequisites to a study of the vegetative buds on the rhizomes of quackgrass is the development of a method in which the rhizomes may be grown where bud growth can be observed easily. Since buds on the rhizomes of quackgrass are subterranean and since immediate observations of growth responses are virtually impossible, it was concluded that a method should be devised which would provide in vitro conditions favorable for growth. Changes in bud activity or dormancy could then be followed throughout the growing season. The method should also be such that observations of and treatments to the rhizomes could be made without disturbing them. Several methods have been used previously, but they do not lend themselves readily to observation or treatment.

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References

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1. Dexter, S. T. Seasonal variations in drought resistance of exposed rhizomes of quackgrass. J. Am. Soc. Agron. 34: 11251136. 1942.
2. Meyer, R. E., and Buchholtz, K. P. Some factors affecting the activity and growth of buds on quackgrass rhizomes. Proc. NCWCC 17:37. 1960.
3. Skoog, F. Growth and organ formation in tobacco tissue cultures. Am. J. Bot. 31:1924. 1944.
4. Skoog, F. and Tsui, Cheng. Chemical control of growth and bud formation in tobacco stem segments and callus cultured in vitro. Am. J. Bot. 35:782787. 1948.
5. White, P. R. Accessory salts in the nutrition of excised roots. Plant Physiol. 13:391398. 1938.

An in vitro Method of Evaluating the Activity of Buds on the Rhizomes of Quackgrass (Agropyron repens)

  • B. G. Johnson (a1) and K. P. Buchholtz (a1)

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