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Separation of Glyphosate and Possible Metabolites by Thin-Layer Chromatography

Published online by Cambridge University Press:  12 June 2017

Paul Sprankle ,
C. L. Sandberg ,
W. F. Meggitt  and
Donald Penner
Paul Sprankle
Affiliation:
Dep. Crop and Soil Sci., Michigan State Univ., East Lansing, MI 48824
C. L. Sandberg
Affiliation:
Dep. Crop and Soil Sci., Michigan State Univ., East Lansing, MI 48824
W. F. Meggitt
Affiliation:
Dep. Crop and Soil Sci., Michigan State Univ., East Lansing, MI 48824
Donald Penner
Affiliation:
Dep. Crop and Soil Sci., Michigan State Univ., East Lansing, MI 48824
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Abstract

Glyphosate [N-(phosphonomethyl)glycine] was separated from its potential metabolites, aminomethylphosphonic acid, glycine, and sarcosine by using 500 μm-thick cellulose plates developed with ethanol:water:15 N NH4OH:trichloroacetic acid (TCA):17 N acetic acid (55:35: 2.5:3.5 g:2, v/v/v/w/v with v in ml). This TLC system separated impurities from the 14C-glyphosate standard and glyphosate from possible metabolites in treated field bindweed (Convolvulus arvensis L.).

Keywords

Glycineaminomethylphosphonic acidsacrosineConvolvulus arvensis
Type
Research Article
Information
Weed Science , Volume 26 , Issue 6 , November 1978 , pp. 673 - 674
Copyright
Copyright © 1978 by the Weed Science Society of America 

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References

Literature Cited

1. Rueppel, M. L., Marvel, J. T., and Suba, L. A. 1975. The metabolism of N-phosphonomethylglycine in corn, cotton, soybeans, and wheat. Am. Chem. Soc. Div., Pestic. Chem. Abstr. pp. 8–9.Google Scholar
2. Sprankle, P., Meggitt, W. F., and Penner, D. 1975. Rapid inactivation of glyphosate in the soil. Weed Sci. 23:224–228.Google Scholar