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Prevalence of a Novel Resistance Mechanism to PPO-Inhibiting Herbicides in Waterhemp (Amaranthus tuberculatus)

Published online by Cambridge University Press:  20 January 2017

Ryan M. Lee ,
Aaron G. Hager  and
Patrick J. Tranel
Ryan M. Lee
Affiliation:
Department of Crop Sciences, University of Illinois, 1201 West Gregory Drive, Urbana, IL 61801
Aaron G. Hager
Affiliation:
Department of Crop Sciences, University of Illinois, 1201 West Gregory Drive, Urbana, IL 61801
Patrick J. Tranel*
Affiliation:
Department of Crop Sciences, University of Illinois, 1201 West Gregory Drive, Urbana, IL 61801
*
Corresponding author's E-mail: tranel@uiuc.edu
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Abstract

Resistance to protoporphyrinogen oxidase (PPO)-inhibiting herbicides in waterhemp has been shown previously to be the result of a unique mechanism. Specifically, a three–base-pair (3-bp) deletion in the PPX2L gene, a gene encoding both plastid- and mitochondria-targeted PPO enzymes, confers herbicide resistance in this species. Furthermore, when this unique mechanism was initially characterized it was presumed that waterhemp contained three PPX genes, PPX1, PPX2S, and PPX2L, and that the resistant biotypes were missing PPX2S. Here, allele testing and examination of genetic sequence data demonstrate that there are likely only two PPX genes in waterhemp, PPX1 and PPX2L. Next, to determine the prevalence of this mechanism of resistance in Illinois waterhemp, we developed an allele-specific polymerase chain reaction (PCR) marker that amplifies only the 3-bp deletion allele, ΔG210, of PPX2L. By utilizing this marker, we show that the ΔG210 PPX2L allele correlated with whole-plant resistance to PPO inhibitors in each of four other waterhemp populations evaluated from Illinois.

Keywords

Common waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea & Tardif AMATUtall waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. tuberculatus (Sauer) Costea & Tardif AMATU.Herbicide resistanceprotoporphyrinogen oxidaseprotoxmolecular marker
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 3 , June 2008 , pp. 371 - 375
Copyright
Copyright © Weed Science Society of America 

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