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Multiple Pro197 ALS Substitutions Endow Resistance to ALS Inhibitors within and among Mayweed Chamomile Populations

Published online by Cambridge University Press:  20 January 2017

Suphannika Intanon ,
Alejandro Perez-Jones ,
Andrew G. Hulting  and
Carol A. Mallory-Smith
Suphannika Intanon*
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
Alejandro Perez-Jones
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
Andrew G. Hulting
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
Carol A. Mallory-Smith
Affiliation:
Department of Crop and Soil Science, Oregon State University, 107 Crop Science Building, Corvallis, OR 97331
*
Corresponding author's E-mail: suphannika.intanon@oregonstate.edu
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Abstract

Mayweed chamomile seeds were collected from six different fields across the Pacific Northwest. All populations (each collection site was considered a population) were suspected to have some level of acetolactate synthase (ALS) resistance. Greenhouse and laboratory studies were conducted to determine if these populations were resistant to three different classes of ALS inhibitors: sulfonylureas (SU), imidazolinones (IMI), and triazolopyrimidines (TP). A whole-plant dose–response and in vitro ALS activity studies confirmed cross-resistance to thifensulfuron + tribenuron/chlorsulfuron (SU), imazethapyr (IMI), and cloransulam (TP); however, resistance varied by herbicide class and population. Two ALS isoforms of the ALS gene (ALS1 and ALS2) were identified in mayweed chamomile; however, only mutations in ALS1 were responsible for resistance. No mutations were found in ALS2. Sequence analysis of the partial ALS gene identified four point mutations at position 197 (Pro197 to Leu, Gln, Thr, or Ser) in the resistant populations. This study demonstrates genotypic variation associated with cross-resistance to ALS inhibitors within and between populations.

Keywords

Thifensulfuron +tribenuronchlorsulfuronimazethapyrcloransulammayweed chamomile, Anthemis cotula L. ANTCOwheat, Triticum aestivum L. TRZAXALS inhibitorsALSherbicide resistancemayweed chamomiletarget-site
Type
Weed Management
Information
Weed Science , Volume 59 , Issue 3 , September 2011 , pp. 431 - 437
Copyright
Copyright © Weed Science Society of America 

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