Hostname: page-component-7c8c6479df-r7xzm Total loading time: 0 Render date: 2024-03-29T16:01:34.778Z Has data issue: false hasContentIssue false

Mechanism of resistance to clethodim in a johnsongrass (Sorghum halepense) biotype

Published online by Cambridge University Press:  20 January 2017

Ian C. Burke
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
James D. Burton
Affiliation:
Department of Horticulture, North Carolina State University, Raleigh, NC 27695-7609
Alan C. York
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
John Cranmer
Affiliation:
Valent USA Corporation, Suite 201, 110 Iowa Lane, Cary, NC 27511

Abstract

A biotype of johnsongrass cross resistant to clethodim, sethoxydim, quizalofop-P, and fluazifop-P was identified in several fields in Washington County, MS. Absorption, translocation, and metabolism studies using 14C-clethodim and acetyl-coenzyme A carboxylase (ACCase) activity assays were conducted to determine the resistance mechanism. Absorption of 14C-clethodim was higher in the resistant than the susceptible biotype 4 hours after treatment (HAT), but at 24, 48, and 72 HAT, similar levels of radioactivity were detected in both johnsongrass biotypes. Consequently, resistant plants had more radioactivity present in the treated leaves at 4 and 24 HAT. However, there was no difference between resistant and susceptible biotypes in the translocation of 14C out of the treated leaf at 4, 8, 24, 48, and 72 HAT as a percentage of total absorbed. Metabolism of clethodim was similar in the resistant and susceptible biotypes. There was no difference in the specific activity of ACCase from the susceptible and resistant johnsongrass biotypes (means of 0.221 and 0.223 nmol mg−1 protein min−1, respectively). ACCase from the susceptible biotype was sensitive to clethodim, with an I50 value of 0.29 μM clethodim. The ACCase enzyme from the resistant biotype was less sensitive, with an I50 value of 1.32 μM clethodim. The resultant R/S ratio for clethodim was 4.5. These results indicate that resistance to clethodim in this johnsongrass biotype resulted from an altered ACCase enzyme that confers resistance to clethodim.

Type
Physiology, Chemistry, and Biochemistry
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Anonymous. 2005. Select 2EC® . Pages 18881890 in Crop Protection Reference. 21st ed. New York: C & P.Google Scholar
Askew, S. D. and Wilcut, J. W. 2002. Absorption, translocation, and metabolism of foliar-applied CGA 362622 in cotton, peanut, and selected weeds. Weed Sci 50:293298.CrossRefGoogle Scholar
Bourgeois, L., Kenkel, N. C., and Morrison, I. N. 1997. Characterization of cross-resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua). Weed Sci 45:750755.CrossRefGoogle Scholar
Bradley, K. W., Wu, J., Hatzios, K. K., and Hagood, E. S. Jr. 2001. The mechanism of resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in a johnsongrass biotype. Weed Sci 49:477484.CrossRefGoogle Scholar
Burke, I. C., Price, A. J., Wilcut, J. W., Jordan, D. L., Culpepper, A. S., and Tredaway-Ducar, J. 2004. Annual grass control in peanut (Arachis hypogaea) with clethodim and imazapic. Weed Technol 18:8892.CrossRefGoogle Scholar
Burke, I. C. and Wilcut, J. W. 2003a. Physiological basis for antagonism of clethodim by CGA 362622. Weed Sci 51:671677.CrossRefGoogle Scholar
Burke, I. C. and Wilcut, J. W. 2003b. Physiological basis for antagonism of clethodim by imazapic on goosegrass [Eleusine indica L. Gaertn]. Pest. Biochem. Physiol 76:3745.CrossRefGoogle Scholar
Burke, I. C., Wilcut, J. W., and Cranmer, J. 2006. Cross resistance of a johnsongrass (Sorghum halepense) biotype to aryloxyphenoxypropionate and cyclohexanedione herbicides. Weed Technol. In press.CrossRefGoogle Scholar
Burke, I. C., Wilcut, J. W., and Porterfield, D. 2003. CGA-363633 antagonizes annual grass control with clethodim. Weed Technol 16:749754.CrossRefGoogle Scholar
Burton, J. D., Gronwald, J. W., Somers, D. A., Connelly, J. A., Gegenbach, B. G., and Wyse, D. L. 1989. Inhibition of plant acetyl-coenzyme A carboxylase by the herbicides sethoxydim and haloxyfop. Biochem. Biophys. Res. Commun 148:10391044.CrossRefGoogle Scholar
Campbell, J. R. and Penner, D. 1985. Sethoxydim metabolism in monocotyledonous and dicotyledonous plants. Weed Sci 33:771773.CrossRefGoogle Scholar
Campbell, J. R. and Penner, D. 1987. Retention, absorption, translocation, and distribution of sethoxydim in monocotyledonous and dicotyledonous plants. Weed Res 27:179186.CrossRefGoogle Scholar
Christoffers, M. J., Berg, M. L., and Messersmith, C. G. 2002. An isoleucine to leucine mutation in acetyl-CoA carboxylase confers herbicide resistance in wild oat. Genome 45:10491056.CrossRefGoogle ScholarPubMed
Culpepper, A. S., Jordan, D. L., York, A. C., Corbin, F. T., and Sheldon, Y. 1999. Influence of adjuvants and bromoxynil on absorption of clethodim. Weed Technol 13:536541.CrossRefGoogle Scholar
Delye, C., Calmes, E., and Matejicek, A. 2002a. SNP markers for blackgrass (Alopecurus myosuroides Huds) genotypes resistant to acetyl CoA-carboxylase inhibiting herbicides. Theor. Appl. Genet 104:11141120.CrossRefGoogle ScholarPubMed
Delye, C., Matejicek, A., and Gasquez, J. 2002b. PCR-based detection of resistance to acetyl-CoA carboxylase inhibiting herbicides in black-grass (Alopecurus myosuroides Huds.) and ryegrass (Lolium rigidum Gaud). Pest. Manage. Sci 58:474478.CrossRefGoogle ScholarPubMed
Delye, C., Straub, C., Matejicek, A., and Michel, S. 2003. Multiple origins for black-grass (Alopecurus myosuroides Huds) target-site-based resistance to herbicides inhibiting acetyl-CoA carboxylase. Pest Manage. Sci 60:3541.CrossRefGoogle Scholar
Delye, C., Wang, T., and Darmency, H. 2002c. An isoleucine-leucine substitution in chloroplastic acetyl-CoA carboxylase from green foxtail (Setaria viridis (L.) Beauv.) is responsible for resistance to the cyclohexanedione herbicide sethoxydim. Planta 214:421427.CrossRefGoogle Scholar
Devine, M. D. 1997. Mechanism of resistance to acetyl-coenzyme A carboxylase inhibitors: a review. Pestic. Sci 51:259264.3.0.CO;2-S>CrossRefGoogle Scholar
Devine, M. D. and Eberlein, C. V. 1997. Physiological, biochemical, and molecular aspects of herbicide resistance based on altered target sites. Pages 159185 in Roe, R. M., Burton, J. D., and Kuhr, R. J. eds. Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Amsterdam, The Netherlands: IOS Press.Google Scholar
Devine, M. D. and Shimabukuro, R. H. 1994. Resistance to acetyl coenzyme A carboxylase inhibiting herbicides. Pages 141169 in Powles, S. B. and Holtum, J.A.M. eds. Herbicide Resistance: Biology and Biochemistry. Boca Raton, FL: Lewis.Google Scholar
Devine, M. D. and Shulka, A. 2000. Altered target sites as a mechanism of herbicide resistance. Crop Prot 19:881889.CrossRefGoogle Scholar
Draper, N. R. and Smith, H. 1981. Applied Regression Analysis. New York: Wiley. Pp. 3342, 511.Google Scholar
Esau, K. 1977. Anatomy of Seed Plants. New York: John Wiley and Sons. P. 286.Google Scholar
Gronwald, J. W., Eberlein, C. V., Betts, K. J., Baerg, R. J., Ehlke, N. J., and Wyse, D. L. 1992. Mechanism of diclofop resistance in an Italian ryegrass (Lolium multiflorum Lam.) biotype. Pestic. Biochem. Physiol 44:126139.CrossRefGoogle Scholar
Heap, I. 2005. The International Survey of Herbicide Resistant Weeds. Available at www.weedscience.com. Accessed March 17, 2005.Google Scholar
Heap, I. M. and Morrison, I. N. 1996. Resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in green foxtail (Setaria viridis). Weed Sci 44:2530.CrossRefGoogle Scholar
Hidayat, I. and Preston, C. 1997. Enhanced metabolism of fluazifop acid in a biotype of Digitaria sanguinalis resistant to the herbicide fluazifop-P-butyl. Pest. Biochem. Physiol 57:137146.CrossRefGoogle Scholar
Incledon, B. J. and Hall, J. C. 1997. Acetyl-coenzyme A carboxylase: quaternary structure and inhibition by graminicidal herbicides. Pest. Biochem. Physiol 57:255271.CrossRefGoogle Scholar
Konishi, T. and Sasaki, Y. 1994. Compartmentalization of two forms of acetyl-CoA carboxylase in plants and the origin of their tolerance toward herbicides. Proc. Natl. Acad. Sci. USA 911:35983601.CrossRefGoogle Scholar
Leach, G. E., Devine, M. D., Kirkwood, R. C., and Marshall, G. 1995. Target enzyme-based resistance to acetyl-coenzyme A carboxylase inhibitors in Eleusine indica . Pestic. Biochem. Physiol 51:129136.CrossRefGoogle Scholar
MacFadden, J. J., Frear, D. S., and Mansager, E. R. 1989. Aryl hydroxylation of diclofop by a cytochrome P-450 dependent monoxygenase from wheat. Pestic. Biochem. Physiol 34:92100.CrossRefGoogle Scholar
Maneechote, C., Holtum, J. A. M., Preston, C., and Powles, S. B. 1994. Resistant acetyl-CoA carboxylase is a mechanism of herbicide resistance in a biotype of Avena sterilis ssp. ludoviciana . Plant Cell Physiol 35:627635.CrossRefGoogle Scholar
Marles, M. A. S., Devine, M. D., and Hall, J. C. 1993a. Herbicide resistance in Setaria viridis conferred by a less sensitive form of acetyl coenzyme A carboxylase. Pestic. Biochem. Physiol 46:714.CrossRefGoogle Scholar
Marles, M. A. S., Devine, M. D., and Hall, J. C. 1993b. Herbicide resistance in green foxtail (Setaria viridis (L.) Beauv.) and johnsongrass (Sorghum halepense (L.) Pers.) biotypes conferred by an insensitive form of acetyl coenzyme-A carboxylase. Weed Sci. Soc. Am. Abstr 33:62.Google Scholar
McIntosh, M. S. 1983. Analysis of combined experiments. Agron. J 75:153155.CrossRefGoogle Scholar
Preston, C., Tardif, F. J., Christopher, J. T., and Powles, S. B. 1996. Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic. Biochem. Physiol 54:123134.CrossRefGoogle Scholar
[SAS] Statistical Analysis Systems. 1998. SAS/STAT User's Guide. Release 7.00. Cary, NC: Statistical Analysis Systems Institute. 1028 p.Google Scholar
Seefeldt, S. S., Fuerst, E. P., Gealy, D. R., Shukla, A., Irzyk, G. P., and Devine, M. D. 1996. Mechanisms of resistance to diclofop of two wild oat (Avena fatua) biotypes from the Willamette Valley of Oregon. Weed Sci 44:776781.CrossRefGoogle Scholar
Seefeldt, S. S., Jensen, J., and Fuerst, P. 1995. Log-logistic analysis of herbicide dose-response relationships. Weed Technol 9:213412.CrossRefGoogle Scholar
Smeda, R. J., Snipes, C. E., and Barrentine, W. L. 1997. Identification of graminicide-resistant johnsongrass (Sorghum halepense). Weed Sci 45:132137.CrossRefGoogle Scholar
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem 150:7680.CrossRefGoogle ScholarPubMed
Tal, A. and Rubin, B. 2004. Molecular characterization and inheritance of resistance to ACCase-inhibiting herbicides in Lolium rigidum . Pestic. Manage. Sci 60:10131018.CrossRefGoogle ScholarPubMed
Tardif, F. J., Holtum, J. A. M., and Powles, S. B. 1993. Occurrence of a herbicide-resistant acetyl-coenzyme A carboxylase mutant in annual ryegrass (Lolium rigidum) selected by sethoxydim. Planta 190:176181.CrossRefGoogle Scholar
Vidrine, P. R., Reynolds, D. B., and Blouin, D. C. 1995. Grass control in soybean (Glycine max) with graminicides applied alone or in mixtures. Weed Technol 9:6872.CrossRefGoogle Scholar
Volenberg, D. and Stoltenberg, D. 2002. Altered acetyl-coenzyme A carboxylase confers resistance to clethodim, fluazifop, and sethoxydim in Setaria faberi and Digitaria sanguinalis . Weed Res 42:342350.CrossRefGoogle Scholar
Wanamarta, G. and Penner, D. 1989. Identification of efficacious adjuvants for sethoxydim and bentazon. Weed Technol 3:6066.CrossRefGoogle Scholar
Zagnitko, O., Jelenska, J., Tevzadze, G., Haselkorn, R., and Gornicki, P. 2001. An isoleucine/leucine residue in the carboxyltransferase domain of acetyl-CoA carboxylase is critical for interaction with aryloxyphenoxypropionate and cyclohexanedione inhibitors. Proc. Natl. Acad. Sci. USA 98:66176622.CrossRefGoogle ScholarPubMed