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Influence of Amitrole Upon Protein Metabolism in Bean Plants

Published online by Cambridge University Press:  12 June 2017

John C. Brown  and
Mason C. Carter
John C. Brown
Affiliation:
Department of Forestry, Auburn University, Auburn, Alabama
Mason C. Carter
Affiliation:
Department of Forestry, Auburn University, Auburn, Alabama
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Abstract

No effect was shown of 3-amino-l,2,4-triazole (amitrole) upon the incorporation of alanine or histidine into soluble protein in bean (Phaseolus vulgaris L.) hypocotyls grown in darkness. The metabolic derivative of amitrole, β-(3-amino-1,2,4-triazolyl-l-)α-ala-nine (hereinafter referred to as 3-ATAL), also had no effect upon histidine incorporation. Serine incorporation was increased 56% in the presence of amitrole, but this may result from a reduction of endogenous serine pools. No evidence indicates a general disruption of protein synthesis. Activity from amitrole-5-14C was readily incorporated into bean protein, but hydrolysis revealed no 3-ATAL. Most of the activity was recovered as amitrole. Amitrole in the presence of riboflavin and light attacked bovine serum albumin, probably by free radical formation. Hydrolysis of bovine serum albumin revealed mainly amitrole. Apparently, the entry of amitrole into bean protein is by free radical formation.

Type
Research Article
Information
Weed Science , Volume 16 , Issue 2 , April 1968 , pp. 222 - 226
Copyright
Copyright © Weed Science Society of America 

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References

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