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Glyphosate Resistance Is More Variable Than Paraquat Resistance in a Multiple-Resistant Hairy Fleabane (Conyza bonariensis) Population

  • Marcelo L. Moretti (a1), Bradley D. Hanson (a1), Kurt J. Hembree (a2) and Anil Shrestha (a3)

Abstract

Reduced control of some glyphosate-resistant hairy fleabane populations with paraquat has raised concerns about evolved multiple resistance to both glyphosate and paraquat. The objective of this study was to confirm the presence of multiple-resistant (glyphosate and paraquat) hairy fleabane populations in California. A series of dose-response experiments was conducted to evaluate the effect of glyphosate and paraquat in a known susceptible (S) and putative multiple-resistant (R) population of hairy fleabane. The greenhouse experiments were conducted during summer, fall, and winter under controlled temperature and natural light conditions. Multiple-resistant hairy fleabane was identified; however, the level of resistance to glyphosate varied substantially among seasons. During the summer, the glyphosate rate required to reduce growth by 50% (GR50) for the R population was 0.94 kg ae ha−1, 5.2-fold more than for the S population. In the fall and winter experiments, however, the R population response to glyphosate was similar to the S population with a GR50 of 0.22 kg ae ha−1 or less. Multiple-resistant plants were controlled in the fall and winter at rates that did not control the same population during summer. GR50 of paraquat varied among seasons (0.94, 0.24, and 0.07 kg ai ha−1 during summer, fall, and winter, respectively); however, plant mortality was more consistent. This is the first reported case of glyphosate–paraquat resistance in hairy fleabane and the multiple-resistant population could pose a significant challenge to annual no-till and perennial cropping systems in California. Further research on the mechanisms of resistance and the physiological factors underlying the seasonally variable response to glyphosate is needed.

Copyright

Corresponding author

Corresponding author's E-mail: bhanson@ucdavis.edu.

References

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Beckie, H. J. 2011. Herbicide-resistant weed management: focus on glyphosate. Pest Manag. Sci. 67:10371048.
Christoffoleti, P. J., Galli, A.J.B., Carvalho, S.J.P., Moreira, M. S., Nicolai, M., Foloni, L. L., Martins, B.A.B., and Ribeiro, D. N. 2008. Glyphosate sustainability in South American cropping systems. Pest Manag. Sci. 64:422427.
CIMIS. 2012. California Irrigation Management Information System—Department of Water Resources. http://www.cimis.water.ca.gov. Accessed October 15, 2012.
Dinelli, G., Marotti, I., Bonetti, A., Catizone, P., Urbano, J. M., and Barnes, J. 2008. Physiological and molecular bases of glyphosate resistance in Conyza bonariensis biotypes from Spain. Weed Res. 48:257265.
Eubank, T. W., Nandula, V. K., Poston, D. H., and Shaw, D. R. 2012. Multiple resistance of horseweed to glyphosate and paraquat and its control with paraquat and metribuzin combinations. Agronomy 2:358370.
Feng, P.C.C., Tran, M., Chiu, T., Sammons, R. D., Heck, G. R., and Jacob, C. A. 2004. Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci. 52:498505.
Fuerst, E. P., Nakatani, H. Y., Dodge, A. D., Penner, D., and Arntzen, C. J. 1985. Paraquat resistance in Conyza . Plant Physiol. 77:984989.
Ge, X., d'Avignon, D. A., Ackerman, J.J.H., Duncan, B., Spaur, M. B., and Sammons, R. D. 2011. Glyphosate resistant horseweed made sensitive to glyphosate: low temperature suppression of glyphosate vacuolar sequestration revealed by 31P NMR. Pest Manag. Sci. 67:12151221.
Ge, X., d'Avignon, D. A., Ackerman, J.J.H., and Sammons, R. D. 2010. Rapid vacuolar sequestration: the horseweed glyphosate resistance mechanism. Pest Manag. Sci. 66:345348.
Heap, I. M. 2012. The International Survey of Herbicide-Resistant Weeds. http://www.weedscience.com. Accessed October 14, 2012.
Jóri, B., Soós, V., Szego, D., Páldi, E., Szigeti, Z., Rácz, I., and Lásztity, D. 2007. Role of transporters in paraquat resistance of horseweed (Conyza canadensis (L.) Cronq). Pestic. Biochem. Physiol. 88:5765.
Koger, C. H. and Reddy, K. N. 2005. Role of absorption and translocation in the mechanism of glyphosate resistance in horseweed (Conyza canadensis). Weed Sci. 53:8489.
McCullagh, P. and Nelder, J. A. 1989. Generalized Linear Models. 2nd ed. London, England Chapman and Hall. 511 p.
Moretti, M. L. 2011. Determination of multiple herbicide resistance in populations of hairy fleabane (Conyza bonariensis) in the Central Valley of California and evaluation of alternative chemical control. . Fresno, CA: California State University.109 p.
Nol, N., Tsikou, D., Eid, M., Livieratos, I., and Giannopolitis, C. 2012. Shikimate leaf disc assay for early detection of glyphosate resistance in Conyza canadensis and relative transcript levels of EPSPs and ABC transporter genes. Weed Res. 52:233241.
Norman, M. A., Smeda, R. J., Vaughn, K. C., and Fuerst, E. P. 1994. Differential movement of paraquat in resistant and sensitive biotypes of Conyza . Pestic. Biochem. Physiol. 50:3142.
Pratley, J. E., Urwin, N., Stanton, R., Baines, P., Broster, J., Cullis, K., Schafer, D., Bohn, J., and Krueger, R. 1999. Resistance to glyphosate in Lolium rigidum. I. Bioevaluation. Weed Sci. 47:405411.
Purba, E., Preston, C., and Powles, S. B. 1995. The mechanism of resistance to paraquat is strongly temperature dependent in resistant Hordeum leporinum Link and H. glaucum Steud. Planta 196:464468.
SAS Institute. 2012. Modeling and Multivariate Methods. JMP® 10. Cary, NC SAS Institute, Inc. 712 p.
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicide dose-response relationships. Weed Technol. 9:218227.
Shaner, D. L. 2009. Role of translocation as a mechanism of resistance to glyphosate. Weed Sci. 57:118123.
Shrestha, A., Hanson, B. D., and Hembree, K. J. 2008. Glyphosate-resistant hairy fleabane documented in the Central Valley. Calif. Agric. 62:116119.
Travlos, I. S. and Chachalis, D. 2010. Glyphosate-resistant hairy fleabane (Conyza bonariensis) is reported in Greece. Weed Technol. 24:569573.
Urbano, J. M., Borrego, A., Torres, V., Leon, J. M., Jimenez, C., Dinelli, G., and Barnes, J. 2007. Glyphosate-resistant hairy fleabane (Conyza bonariensis) in Spain. Weed Technol. 21:396401.
Varadi, G., Darko, E., and Lehoczki, E. 2000. Changes in the xanthophyll cycle and fluorescence quenching indicate light-dependent early events in the action of paraquat and the mechanism of resistance to paraquat in Erigeron canadensis (L.) Cronq. Plant Physiol. 123:14591469.
Yu, Q., Cairns, A., and Powles, S. 2007. Glyphosate, paraquat and ACCase multiple herbicide resistance evolved in a Lolium rigidum biotype. Planta 225:499513.
Yuan, J. S., Abercrombie, L.L.G., Cao, Y., Halfhill, M. D., Zhou, X., Peng, Y., Hu, J., Rao, M. R., Heck, G. R., Larosa, T. J., Sammons, R. D., Wang, X., Ranjan, P., Johnson, D. H., Wadl, P. A., Scheffler, B. E., Rinehart, T. A., Trigiano, R. N., and Stewart, C. N. Jr. 2010. Functional genomics analysis of horseweed (Conyza canadensis) with special reference to the evolution of non–target-site glyphosate resistance. Weed Sci. 58:109117.
Yuan, J. S., Tranel, P. J., and Stewart, C. N. Jr. 2007. Non-target-site herbicide resistance: a family business. Trends Plant Sci. 12:613.

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