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Effects of Shading on the Anatomy and Ultrastructure of the Leaf Mesophyll and Vascular Bundles of Itchgrass (Rottboellia exaltata)

Published online by Cambridge University Press:  12 June 2017

Rex N. Paul  and
David T. Patterson
Rex N. Paul
Affiliation:
South. Weed Sci. Lab., Agric. Res., Sci. Ed. Admin., U.S. Dep. Agric., Stoneville, MS 38776
David T. Patterson
Affiliation:
South. Weed Sci. Lab., Agric. Res., Sci. Ed. Admin., U.S. Dep. Agric., Stoneville, MS 38776
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Abstract

A study of the C4 plant itchgrass (Rottboellia exaltata L.f.) grown under 100%, 60%, 25% and 2% sunlight revealed differences in the anatomy and cytology of the foliar mesophyll and vascular bundles associated with shading. In the bundle sheath, shading caused a reduction in thickness of the cell walls, shrinkage of plastids, rearrangement of plastid thylakoids, a reduction in starch deposits and vacuolization of the cytoplasm. In general, plastids and mitochondria retained membrane integrity but underwent stromal deterioration. Shading effects on cytoplasm were similar for mesophyll and bundle sheath cells. Mesophyll chloroplasts lost starch grains and the peripheral reticulum tended to decrease with greater shading. Grana were well developed at all irradiances, although the chloroplasts themselves decreased in size with shading. Movement of mesophyll chloroplasts away from bundle sheath-mesophyll borders was pronounced at 60% sunlight and was progressively greater at the two lower irradiances. These observations suggest that the structural relationships thought to be necessary for the intercellular transfer of C4 acids and the functioning of the C4 photosynthetic pathway were disrupted by shading.

Keywords

Bundle sheathleaf mesophyllchloroplastsC4-photosynthesisKranz anatomy
Type
Research Article
Information
Weed Science , Volume 28 , Issue 2 , March 1980 , pp. 216 - 224
Copyright
Copyright © 1980 by the Weed Science Society of America 

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References

Literature Cited

1. Bjorkman, O. and Holmgren, P. 1963. Adaptability of the photosynthetic apparatus to light intensity in ecotypes from exposed and shaded habitats. Physiol. Plant. 16:889–914.Google Scholar
2. Boardman, K. 1977. Comparative photosynthesis of sun and shade plants. Annu. Rev. Plant Physiol. 28:355–377.Google Scholar
3. Brangeon, J. 1973. Effect of irradiance on granal configurations of Zea mays bundle sheath chloroplasts. Photosynthetica 7:365–372.Google Scholar
4. Brown, W. V. 1975. Variations in anatomy, associations, and origins of Kranz tissue. Am. J. Bot. 62:395–402.CrossRefGoogle Scholar
5. Carmer, S. G. and Swanson, M. R. 1971. Detection of differences between means: a Monte Carlo study of five pairwise multiple comparison procedures. Agron. J. 63:940–945.CrossRefGoogle Scholar
6. Carolin, R. C., Jacobs, S. W. L., and Vesk, M. 1973. The structure of the cells of the mesophyll and parenchymatous bundle sheath of the Gramineae. Bot. J. Linn. Soc. 66:259–275.Google Scholar
7. Carolin, R. C., Jacobs, S. W. L., and Vesk, M. 1977. The ultrastructure of Kranz cells in the family Cyperaceae. Bot. Gaz. 138:413–319.Google Scholar
8. Chabot, B. F. and Chabot, J. F. 1977. Effects of light and temperature on leaf anatomy and photosynthesis in Fragaria vesca . Oecologia (Berlin) 26:363–377.CrossRefGoogle ScholarPubMed
9. Downton, W. J. S. 1970. Preferential C4-dicarboxylic acid synthesis, the postillumination CO2 burst, carboxyl transfer step, and grana configurations in plants with C4-photosynthesis. Can. J. Bot. 48:1795–1800.Google Scholar
10. Hatch, M. D. 1976. Photosynthesis: The path of carbon. Pages 797–844 in Bonner, J. and Varner, J., eds. Plant Biochemistry. Academic Press, New York.Google Scholar
11. Hatch, M. D., Kagawa, T., and Craig, S. 1975. Subdivision of C4 acid decarboxylating systems and ultrastructural features. Aust. J. Plant Physiol. 2:111–128.Google Scholar
12. Holm, L. G., Plucknett, D. L., Pancho, J. V., and Herberger, J. P. 1977. The World's Worst Weeds: Distribution and Biology. Univ. Press of Hawaii, Honolulu. 609 pp.Google Scholar
13. Karnovsky, M. J. 1965. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27:137A–138A.Google Scholar
14. Kramer, P. J., Hellmers, H., and Downs, R. J. 1970. SEPEL: New phytotrons for environmental research. Bioscience 20:1201–1208.CrossRefGoogle Scholar
15. Laetsch, W. M. 1971. Chloroplast relationships in leaves of C4 plants. Pages 323–349 in Hatch, M. D., Osmond, C. B., and Slatyer, R. D., eds. Photosynthesis and Photorespiration. Wiley, New York.Google Scholar
16. Laetsch, W. M. 1974. The C4 syndrome: A structural analysis. Annu. Rev. Plant Physiol. 25:27–52.Google Scholar
17. Louwerse, W. and Zweerde, W. v.d. 1977. Photosynthesis, transpiration and leaf morphology of Phaseolus vulgaris and Zea mays grown at different irradiances in artificial and sunlight. Photosynthetica 11(1):11–21.Google Scholar
18. Patterson, D. T. 1979. The effects of shading on the growth and photosynthetic capacity of itchgrass (Rottboellia exaltata . Weed Sci. 27:549–553.Google Scholar
19. Patterson, D. T. 1979. Light and temperature adaptation. Chapter X in Hesketh, J. D. and Jones, J. W., eds. Predicting Photosynthate Production and Use for Ecosystem Models. CRC Press, West Palm Beach. (In press).Google Scholar
20. Patterson, D. T., Bunce, J. A., Alberte, R. S., and van Volkenburgh, E. 1977. Photosynthesis in relation to leaf characteristics of cotton from controlled and field environments. Plant Physiol. 59: 384–387.Google Scholar
21. Patterson, D. T. and Duke, S. O. 1979. Effect of growth irradiance on the maximum photosynthetic capacity of water hyacinth [Eichornia crassipes (Mart.) Solms]. Plant Cell Physiol. 20:177–184.Google Scholar
22. Patterson, D. T., Duke, S. O., and Hoagland, R. E. 1978. Effect of irradiance during growth on adaptive photosynthetic characteristics of velvetleaf and cotton. Plant Physiol. 61:402–405.CrossRefGoogle ScholarPubMed
23. Patterson, D. T., Longstretch, D. J., and Peet, M. M. 1977. Photosynthetic adaptation to light intensity in Sakhalin knotweed (Polygonum sachalinense . Weed Sci. 25:319–323.Google Scholar
24. Patterson, D. T., Meyer, C. R., Flint, E. P., and Quimby, P. C. Jr. 1979. Temperature response and potential distribution of itchgrass (Rottboellia exaltata L.f.) in the United States. Weed Sci. 27:77–82.Google Scholar
25. Patterson, D. T., Meyer, C. R., and Quimby, P. C. Jr. 1978. Effects of irradiance on relative growth rates, net assimilation rates, and leaf area partitioning in cotton and three associated weeds. Plant Physiol. 62:14–17.CrossRefGoogle ScholarPubMed
26. Reynolds, E. S. 1963. The use of lead citrate as high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17: 208–213.Google Scholar
27. Spurr, A. R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26:31–43.Google Scholar