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Effects of Haloxyfop on Corn (Zea mays) and Soybean (Glycine max) Cell Suspension Cultures

Published online by Cambridge University Press:  12 June 2017

Hyung-Yul Cho ,
Jack M. Widholm  and
Fred W. Slife
Hyung-Yul Cho
Affiliation:
Dep. Agron., Univ. Illinois, 1102 S. Goodwin Avenue, Urbana, IL 61801
Jack M. Widholm
Affiliation:
Dep. Agron., Univ. Illinois, 1102 S. Goodwin Avenue, Urbana, IL 61801
Fred W. Slife
Affiliation:
Dep. Agron., Univ. Illinois, 1102 S. Goodwin Avenue, Urbana, IL 61801
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Abstract

To investigate the mode of action of a grass-specific herbicide, haloxyfop {2-[4-[[3-chloro-5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoic acid} was added to corn and soybean cell suspension cultures containing metabolic intermediates such as sucrose [14C-U], sodium acetate [14C-1,2], or L-leucine [14C-U]. Death of the corn suspension cells occurred within 3 days after haloxyfop was added at a concentration of 1.0 μM to supplemented Murashige and Skoog's medium. At 0.1 μM haloxyfop, 14C-sucrose uptake and 14C loss due to respiration of the corn cells declined without a significant reduction in cell fresh weight, cell viability, cellular ATP level, free sugar content, cell wall materials, or level of glycolytic intermediates. The proportions of sucrose, glucose, and fructose in the free sugar fraction were not affected. However, the 14C-labeled free amino acid level was increased. During a 24-h incubation period, incorporation of leucine [14C] as a precursor revealed that haloxyfop did not inhibit protein synthesis. Incorporation of acetate [14C-1,2] showed that total lipid synthesis was inhibited by 42% at 0.1 μM haloxyfop (sublethal). Similar responses were observed in the soybean suspension cells; however, the calculated 50% lethal concentration (LC50) was 47 times higher than for corn cells. The results of this study suggest that the site of action of haloxyfop is located where metabolic intermediates enter the Kreb's cycle and lipid synthesis. Glycolysis is not inhibited.

Keywords

Dowco 453mode of action
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 34 , Issue 4 , July 1986 , pp. 496 - 501
Copyright
Copyright © 1986 by the Weed Science Society of America 

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References

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