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Calcium may mediate auxinic herbicide resistance in wild mustard

  • Youlin Wang (a1), Satish Deshpande (a1) and J. Christopher Hall


The role of calcium in mediating resistance to several auxinic herbicides (i.e., 2,4-dichlorophenoxyacetic acid, [4-chloro-2-methylphenoxy] acetic acid, (±)-2-(4-chloro-2-methylphenoxy) propanoic acid [mecoprop], 3,6-dichloro-2-methoxy-benzoic acid [dicamba], or 4-amino-3, 5,6-trichloro-2-pyridinecarboxylic acid [picloram]) was investigated by modulating calcium dynamics of a susceptible (S) and resistant (R) biotype of wild mustard. The inhibitory effects of the auxinic herbicides on root length of the S seedlings were significantly reduced upon pretreatment with calcium in the presence of the calcium ionophore A23187. Conversely, the addition of verapamil, a calcium channel blocker, to the R seedlings increased their sensitivity to the auxinic herbicides. Valinomycin, a potassium channel ionophore, did not ameliorate the effect of the auxinic herbicides on both biotypes of wild mustard, thus indicating that the observed effects were specific for calcium. These results demonstrate that calcium plays a crucial role in the resistance of wild mustard to auxinic herbicides at the level of intact seedlings, thereby supporting our previous results using intact protoplasts.


Corresponding author

Corresponding author. Department of Environmental Biology, The University of Guelph, Guelph, Ontario N1G 2W1, Canada;


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Bush, D. S. 1995. Calcium regulation in plant cells and its role in signaling. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46:95122.
Bush, D. S., Biswas, A. K., and Jones, R. L. 1993. Hormonal regulation of Ca2+ transport in the endomembrane system of the aleurone. Planta 189:507515.
Cho, H.-T. and Hong, Y.-N. 1996. Effect of calcium channel blockers on the IAA-induced cell elongation of sunflower hypocotyl segments. J. Plant Physiol. 149:377383.
Coupland, D. 1994. Resistance to the auxin analog herbicides. Pages 171214 In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants. London: Lewis.
Deshpande, S. and Hall, J. C. 1995. Comparison of flash-induced light scattering transients and proton efflux from auxinic-herbicide resistant and susceptible wild mustard protoplasts: a possible role for calcium in mediating auxinic herbicide resistance. Biochem. Biophys. Acta 1244:6978.
Deshpande, S. and Hall, J. C. 1996. ATP-dependent auxin- and auxinic-herbicide induced volume changes in isolated protoplast suspensions from Sinapis arvensis L. Pestic. Biochem. Physiol. 56:2643.
Devine, M. D., Duke, S. O., and Fedtke, C. 1993. Herbicides with auxin activity. Pages 295309 In Physiology of Herbicide Action. New Jersey: Prentice Hall.
Gelli, A., Higgins, V. J., and Blumwald, E. 1997. Activation of plant membrane Ca2+-permeable channels by race-specific fungal elicitors. Plant Physiol. 113:269279.
Gilroy, S. and Jones, R. L. 1993. Calmodulin stimulation of unidirectional calcium uptake by the endoplasmic reticulum of barley aleurone. Planta 190:289296.
Gong, M., Chen, S. N., Song, Y. Q., and Li, Z. G. 1997a. Effect of calcium and calmodulin on intrinsic heat tolerance in maize seedlings in relation to antioxidant system. Aust. J. Plant Physiol. 24:371379.
Gong, M., Li, Y. J., Dai, X., Tian, M., and Li, Z. G. 1997b. Involvement of calcium and calmodulin in the acquisition of heat-shock induced thermotolerance in maize seedlings. J. Plant Physiol. 150:615621.
Gong, M., Van der Luit, A. H., Knight, M. R., and Trewavas, A. J. 1998. Heat-shock-induced changes in intracellular Ca2+ level in tobacco seedlings in relation to thermotolerance. Plant Physiol. 116:429437.
Hall, J. C., Alam, S.M.M., and Murr, D. P. 1993. Ethylene biosynthesis following foliar application of picloram to biotypes of wild mustard (Sinapis arvensis L.) susceptible or resistant to auxinic herbicides. Pestic. Biochem. Physiol. 47:3643.
Hall, J. C. and Romano, M. L. 1995. Morphological and physiological differences between the auxinic herbicide susceptible (S) and resistant (R) wild mustard (Sinapis arvensis L.) biotypes. Pestic. Biochem. Physiol. 52:149155.
Heap, I. 2000. International survey of herbicide resistant weeds. Available at Accessed July 27, 2000.
Heap, I. and Morrison, I. M. 1992. Resistance to auxin-type herbicides in wild mustard (S. arvensis L.) populations in Western Canada. Weed Sci. Am. 32:55 [Abstract].
Hubert, J. J. 1992. Bioassay. 3rd ed. Dubuque, IA: Kendall/Hunt.
Knight, M. R., Campbell, A. K., Smith, S. M., and Trewavas, A. J. 1991. Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium. Nature 352:524526.
Knight, M. R., Read, N. D., Campbell, A. K., and Trewavas, A. J. 1993. Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins. J. Cell Biol. 121:8390.
Knight, M. R., Smith, S. M., and Trewavas, A. J. 1992. Wind-induced plant motion immediately increases cytosolic calcium. Proc. Nat. Acad. Sci. USA 89:49674971.
Latimer, P. 1982. Light scattering absorption as methods of studying cell population parameters. Ann. Rev. Biophys. Bioeng. 11:129150.
Peniuk, M. G., Romano, M. L., and Hall, J. C. 1993. Physiological investigations into the resistance of a wild mustard (Sinapis arvensis L.) biotype to auxinic herbicides. Weed Res. 33:431440.
Poovaiah, B. W. and Reddy, A.S.N. 1993. Calcium and signal transduction in plants. Crit. Rev. Plant Sci. 12:185211.
Price, A. H., Taylor, A., Ripley, S. J., Griffiths, A., Trewavas, A. J., and Knight, M. R. 1994. Oxidative signals in tobacco increase cytosolic calcium. Plant Cell 6:13011310.
Sterling, T. M. and Hall, J. C. 1997. Mechanism of action of natural auxins and the auxinic herbicides. Pages 111141 In Roe, R. M., Burton, J. D., and Kuhr, R. J., eds. Herbicide Activity: Biochemistry and Molecular Biology. Amsterdam: IOS Press.
Tavernier, E., Wendehenne, D., Blein, J.-P., and Pugin, A. 1995. Involvement of free calcium in action of cryptogein, a proteinaceous elicitor of hypersensitive reaction in tobacco cells. Plant Physiol. 109:10251031.
Webb, S. R. and Hall, J. C. 1995. Auxinic-herbicide resistant and susceptible wild mustard (Sinapis arvensis L.) biotypes: effects of auxinic herbicides on seedling growth and auxin-binding activity. Pestic. Biochem. Physiol. 52:137148.
Yang, T. and Poovaiah, B. W. 2000. Molecular and biochemical evidence for the involvement of calcium/calmodulin in auxin action. J. Biol. Chem. 275:31373143.


Calcium may mediate auxinic herbicide resistance in wild mustard

  • Youlin Wang (a1), Satish Deshpande (a1) and J. Christopher Hall


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