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Biology and Management of Glyphosate-Resistant and Glyphosate-Susceptible Palmer Amaranth (Amaranthus palmeri) Phenotypes from a Segregating Population

  • Sushila Chaudhari (a1), David L. Jordan (a1), Alan C. York (a1), Katherine M. Jennings (a2), Charles W. Cahoon (a3), Aman Chandi (a4) and Matthew D. Inman (a1)...


Palmer amaranth is the most economically damaging glyphosate-resistant (GR) weed in the southern United States. An understanding of the basic biology, including relative growth and competitiveness of GR and glyphosate-susceptible (GS) Palmer amaranth phenotypes from a segregating population collected from the same geographical location, may yield information helpful in the management of resistant populations. A segregating population of Palmer amaranth collected in North Carolina during 2010 was used as a plant source for both GR and GS traits. Research was conducted in the greenhouse to compare the following: level of resistance and shikimate accumulation in GR and GS phenotypes following glyphosate application; interference from GR and GS phenotypes on early-season vegetative growth of corn, cotton, and peanut; effect of various durations of imposed drought stress on GR and GS phenotypes; and response of GR and GS phenotypes to POST-applied herbicides. The GR50 (glyphosate rate providing 50% reduction in shoot dry biomass) was 17 times greater with the GR phenotype compared with the GS phenotype. Shikimate accumulated in both GR and GS phenotypes following glyphosate application, but greater concentrations were found in GS plants. The GR and GS phenotypes responded similarly when subjected to drought stress; grown with corn, cotton, and peanut; or treated with 2,4-D, atrazine, dicamba, fomesafen, glufosinate, paraquat, tembotrione, and thifensulfuron. These results indicate that in the absence of glyphosate selection pressure, resistance to glyphosate does not influence the growth and competitiveness of GR and GS Palmer amaranth phenotypes collected from the same geographical location.


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Associate Editor for this paper: Muthukumar V. Bagavathiannan, Texas A&M.



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Baucom, RS, Mauricio, R (2004) Fitness costs and benefits of novel herbicide tolerance in a noxious weed. Proc Natl Acad Sci USA 101:1338613390
Bensch, CN, Horak, MJ, Peterson, D (2003) Interference of redroot pigweed (Amaranthus retroflexus), Palmer amaranth (A. palmeri), and common waterhemp (A. rudis) in soybean. Weed Sci 51:3743
Bergelson, J, Purrington, CB (1996) Surveying patterns in the costs of resistance in plants. Am Nat 148:536558
Bond, JA, Oliver, LR, Stephenson IV, DO (2006) Response of Palmer amaranth (Amaranthus palmeri) accessions to glyphosate, fomesafen, and pyrithiobac. Weed Technol 20:885892
Burke, IC, Schroeder, M, Thomas, WE, Wilcut, JW (2007) Palmer amaranth interference and seed production in peanut. Weed Technol 21:367371
Chandi, A, Jordan, DL, York, AC, Burton, J, Milla-Lewis, SR, Spears, J, Whitaker, JR, Wells, R (2013a) Response of herbicide-resistant Palmer amaranth (Amaranthus palmeri) accessions to drought stress. Int J Agron 2013:10.1155/2013/823913
Chandi, A, Jordan, DL, York, AC, Milla-Lewis, SR, Burton, JD, Culpepper, AS, Whitaker, JR (2013b) Interference and control of glyphosate-resistant and -susceptible Palmer amaranth (Amaranthus palmeri) populations under greenhouse conditions. Weed Sci 61:259266
Chandi, A, Milla-Lewis, SR, Giacomini, D, Westra, P, Preston, C, Jordan, DL, York, AC, Burton, JD, Whitaker, JR (2012) Inheritance of evolved glyphosate resistance in a North Carolina Palmer amaranth (Amaranthus palmeri) biotype. Int J Agron 2012:10.1155/2012/176108
Chandi, A, Milla-Lewis, SR, Jordan, DL, York, AC, Burton, JD, Zuleta, MC, Whitaker, JR, Culpepper, AS (2013c) Use of AFLP markers to assess genetic diversity in Palmer amaranth (Amaranthus palmeri) populations from North Carolina and Georgia. Weed Sci 61:136145
Culpepper, AS, Grey, TL, Vencill, WK, Kichler, JM, Webster, TM, Brown, SM, York, AC, Davis, JW, Hanna, WW (2006) Glyphosate resistant Palmer amaranth (Amaranthus palmeri) confirmed in Georgia. Weed Sci 54:620626
Ehleringer, J (1983) Ecophysiology of Amaranthus palmeri, a Sonoran desert summer annual. Oecologia 57:107112
Franssen, AS, Skinner, DZ, Al-Khatib, K, Horak, MJ (2001) Pollen morphological differences in Amaranthus species and interspecific hybrids. Weed Sci 49:732737
Gaines, TA, Shaner, DL, Ward, SM, Leach, JE, Westra, P (2011) Mechanism of resistance of evolved glyphosate-resistant Palmer amaranth (Amaranthus palmeri). J Agric Sci Food Chem 59:58865889
Giacomini, D, Westra, P, Ward, SM (2014) Impact of genetic background in fitness cost studies: an example from glyphosate-resistant Palmer amaranth. Weed Sci 62:2937
Harring, T, Streibig, JC, Husted, S (1998) Accumulation of shikimic acid: a technique for screening glyphosate efficacy. J Agri Food Chem 46:44064412
Heap, I (2016) The International Survey of Herbicide Resistant Weeds. Accessed: December 28, 2016
Henry, WB, Koger, CH, Shaner, DL (2005) Accumulation of shikimate in corn and soybean exposed to various rates of glyphosate. Crop Manage DOI: 10.1094/CM-2005-1123-01-RS
Howell, TA (1990) Grain, dry matter yield relationships for winter wheat and grain sorghum—Southern High Plains. Agron J 82:914918
Isleib, TG, Milla-Lewis, SR, Pattee, HE, Copeland, SC, Zuleta, MC, Shew, BB, Hollowell, JE, Sanders, TH, Dean, LO, Hendrix, KW, Balota, M, Chapin, JW (2011) Registration of ‘Bailey’ peanut. J Plant Regist 5:2739
Keeley, PE, Carter, CH, Thullen, RJ (1987) Influence of planting date on growth of Palmer amaranth (Amaranthus palmeri). Weed Sci 35:199204
Klingaman, TE, Oliver, LR (1994) Palmer amaranth (Amaranthus palmeri) interference in soybeans (Glycine max). Weed Sci 42:523527
Ma, XY, Wu, HW, Jiang, WL, Ma, YJ, Ma, Y (2015) Interference between redroot pigweed (Amaranthus retroflexus L.) and cotton (Gossypium hirsutum L.): growth analysis. PLoS ONE 10:e0130475
Massinga, RA, Curie, RS, Horak, MJ, Boyer, J Jr (2001) Interference of Palmer amaranth in corn. Weed Sci 49:202208
Mohseni-Moghadam, M, Schroeder, J, Ashigh, J (2013) Mechanism of resistance and inheritance in glyphosate resistant Palmer amaranth (Amaranthus palmeri) populations from New Mexico, USA. Weed Sci 61:517525
Morgan, GD, Baumann, PA, Chandler, JM (2001) Competitive impact of Palmer amaranth (Amaranthus palmeri) on cotton (Gossypium hirsutum) development and yield. Weed Technol 15:408412
Mueller, TC, Massey, JH, Hayes, RM, Main, CL, Stewart, CN Jr (2003) Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq.). J Agric Food Chem 51:680684
Nandula, VK, Reddy, KN, Kroger, CH, Poston, DH, Rimando, AM, Duke, SO, Bond, JA, Ribeiro, DN (2012) Multiple resistance to glyphosate and pyrithiobac in Palmer amaranth (Amaranthus palmeri) from Mississippi and response to flumiclorac. Weed Sci 60:179188
Noldin, JA, Chandler, JM, Ketchersid, ML, McCauley, GN (1999) Red rice (Oryza sativa) biology. II. Ecotype sensitivity to herbicides. Weed Technol 13:1924
Norsworthy, JK, Griffith, GM, Scott, RC, Smith, KL, Oliver, LR (2008) Confirmation and control of glyphosate-resistant Palmer amaranth (Amaranthus palmeri) in Arkansas. Weed Technol 22:108113
Padzoldt, WL, Tranel, PJ, Hager, AG (2002) Variable herbicide response among Illinois waterhemp (Amaranthus rudis and A. tuberculatus) populations. Crop Prot 21:707712
Pederson, BP, Neve, P, Andreasen, C, Powles, SB (2007) Ecological fitness of a glyphosate-resistant Lolium rigidum population: growth and seed production along a competition gradient. Basic Appl Ecol 8:258268
Poirier, AH, York, AC, Jordan, DL, Chandi, A, Everman, WJ, Whitaker, JR (2014) Distribution of glyphosate- and thifensulfuron-resistant Palmer amaranth (Amaranthus palmeri) in North Carolina. Int J Agron 2014:10.1155/2014/747810
Purrington, CB, Bergelson, J (1997) Fitness consequences of genetically engineered herbicide and antibiotic resistance in Arabidopsis thaliana . Genetics 145:807814
Riar, DS, Norsworthy, JK, Bond, JA, Bararpour, MT, Wilson, MJ, Scott, RC (2012). Resistance of Echinochloa crus-galli populations to acetolactate synthase-inhibiting herbicides. Int J Agron 2012:10.1155/2012/893953
Roux, F, Gasquez, J, Reboud, X (2004) The dominance of the herbicide resistance cost in several Arabidopsis thaliana mutant lines. Genetics 166:449460
Smith, DT, Baker, RV, Steele, GL (2000) Palmer amaranth (Amaranthus palmeri) impacts on yield, harvesting, and ginning in dryland cotton (Gossypium hirsutum). Weed Technol 14:122126
Sosnoskie, LM, Kichler, JM, Wallace, RD, Culpepper, AS (2011) Multiple resistance in Palmer amaranth to glyphosate and pyrithiobac confirmed in Georgia. Weed Sci 59:321325
Sosnoskie, LM, Webster, TM, Kichler, JM, MacRae, AW, Grey, TL, Culpepper, AS (2012) Pollen-mediated dispersal of glyphosate-resistance in Palmer amaranth under field conditions. Weed Sci 60:366373
Tardif, FJ, Leroux, GD (1991) Response of quackgrass biotypes to glyphosate and quazalofop. Can J Plant Sci 71:803810
Vila-Aiub, MM, Goh, SS, Gaines, TA, Han, H, Busi, R, Yu, Q, Powles, SB (2013) No fitness cost of glyphosate resistance endowed by massive EPSPS gene amplification in Amaranthus palmeri . Planta 239:793801
Ward, SM, Webster, TM, Steckel, LE (2013) Palmer amaranth (Amaranthus palmeri): a review. Weed Technol 27:1227
Warwick, SI, Black, LD (1994) Relative fitness of herbicide-resistant and susceptible populations of weeds. Phytoprotection 75:3749
WebHADSS. (2004). HADSS decision program 2004.0.3. North Carolina Database. Accessed: August 24, 2016
Webster, TM (2005) Weed survey—southern states. Pages 291306 in Proceedings of Southern Weed Science Society. Las Cruces, NM: Southern Weed Science Society
Werle, R, Sandell, LD, Buhler, DD, Hartzler, RG, Lindquist, JL (2014) Predicting emergence of 23 summer annual weed species. Weed Sci 62:267279
Whitaker, JR (2009) Distribution, Biology, and Management of Glyphosate-resistant Palmer Amaranth in North Carolina. Ph.D dissertation. Raleigh, NC: North Carolina State University. 231 p
Whitaker, JR, Burton, JD, York, AC, Jordan, DL, Chandi, A (2013) Physiology of glyphosate-resistant and glyphosate-susceptible Palmer amaranth (Amaranthus palmeri) populations collected from North Carolina. Int J Agron 2013:10.1155/2013/429294
Whitaker, JR, York, AC, Jordan, DL, Culpepper, AS (2010) Palmer amaranth (Amaranthus palmeri) control in soybean with glyphosate and conventional herbicide systems. Weed Technol 24:403410
Whitaker, JR, York, AC, Jordan, DL, Culpepper, AS (2011) Weed management with glyphosate- and glufosinate-based systems in PHY 485 WRF cotton. Weed Technol 25:183191
Van Wychen, L (2016) 2015 Survey of the Most Common and Troublesome Weeds in the United States and Canada. Weed Science Society of America National Weed Survey Dataset. Accessed: December 28, 2016



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