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Physiological basis for resistance to diphenyl ether herbicides in common waterhemp (Amaranthus rudis)

Published online by Cambridge University Press:  20 January 2017

Jianmei Li ,
Reid J. Smeda ,
Kelly A. Nelson  and
Franck E. Dayan
Jianmei Li
Affiliation:
Agronomy Department, 202 Waters Hall, University of Missouri, Columbia, MO 65211
Reid J. Smeda
Affiliation:
Agronomy Department, 202 Waters Hall, University of Missouri, Columbia, MO 65211
Kelly A. Nelson
Affiliation:
Greenley Research Center, P.O. Box 126, University of Missouri, Novelty, MO 63460
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Abstract

Common waterhemp seeds were collected from two Missouri soybean fields where biotypes were not controlled by acifluorfen. Plants grown from these seeds were tested for resistance to the diphenyl ether herbicides acifluorfen and lactofen. Resistance to susceptibility (R/S) ratios, calculated as the ratio of the dose required to inhibit dry weight accumulation by 50% (GR50) in resistant plants to that for susceptible plants, were 9.5 and 11 for the Meadville biotype and 28 and 44 for the Bethel biotype exposed to acifluorfen and lactofen, respectively. Electrolyte leakage assays determined that light-induced lipid peroxidation by acifluorfen was greatest on a control population (Bradford), intermediate for the Meadville biotype, and lowest for the Bethel biotype. Levels of the photodynamic pigment protoporphyrin IX (Proto) accumulating in leaf disks exposed to acifluorfen were much lower in the resistant biotypes than in the susceptible wild type, and the level of Proto accumulation was significantly correlated to the degree of membrane disruption. Although the binding of acifluorfen to protoporphyrinogen oxidase in chloroplasts may have been altered in the resistant biotypes, the molecular and biochemical factors involved in the mechanism of resistance remain to be fully characterized. However, this study establishes that the physiological basis for the evolved resistance to diphenyl ethers in common waterhemp rests on the reduction of Proto accumulation.

Keywords

Acifluorfenlactofencommon waterhemp, Amaranthus rudis Sauer AMATAsoybean, Glycine max (L.) MerrHerbicide resistanceprotoporphyrinogen oxidaseprotoporphyrin accumulationPPOProtox
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 52 , Issue 3 , June 2004 , pp. 333 - 338
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Becerril, J. M. and Duke, S. O. 1989a. Acifluorfen effects on intermediates of chlorophyll synthesis in green cucumber cotyledon tissues. Pestic. Biochem. Physiol 35:119–126.CrossRefGoogle Scholar
Becerril, J. M. and Duke, S. O. 1989b. Protoporphyrin IX content correlates with activity of photobleaching herbicides. Plant Physiol 90:1175–1181.Google Scholar
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal. Biochem 72:248–254.CrossRefGoogle ScholarPubMed
Choi, K. W., Han, O., Lee, H. J., Yun, Y. C., Moon, Y. H., Kim, M., Kuk, Y. I., Han, S. U., and Guh, J. O. 1998. Generation of resistance to the diphenyl ether herbicide, oxyfluorfen, via expression of the Bacillus subtilis protoporphyrinogen oxidase gene in transgenic tobacco plants. Biosci. Biotechnol. Biochem 62:558–560.Google Scholar
Dayan, F. E. and Duke, S. O. 1997. Phytotoxicity of protoporphyrinogen oxidase inhibitors: phenomenology, mode of action and mechanisms of resistance. Pages 11–35 in Roe, R. M., Burton, J. D., and Kuhr, R. J. eds. Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Washington, DC: IOS.Google Scholar
Dayan, F. E., Duke, S. O., Reddy, K. N., Hamper, B. C., and Leschinsky, K. L. 1997a. Effects of isoxazole herbicides on protoporphyrinogen oxidase and porphyrin physiology. J. Agric. Food Chem 45:967–975.CrossRefGoogle Scholar
Dayan, F. E., Duke, S. O., Weete, J. D., and Hancock, H. G. 1997b. Selectivity and mode of action of carfentrazone-ethyl, a novel phenyl triazolinone herbicide. Pestic. Sci 51:65–73.Google Scholar
Dayan, F. E., Meazza, G., Bettarini, F., Signorini, E., Piccardi, P., Romagni, J. G., and Duke, S. O. 2001. Synthesis, herbicidal activity and mode of action of IR 5790. J. Agric. Food Chem 49:2302–2307.Google Scholar
Dayan, F. E., Rimando, A. M., Duke, S. O., and Jacobs, N. J. 1999. Thiol- dependent degradation of protoporphyrin IX by plant peroxidases. FEBS Lett 444:227–230.CrossRefGoogle ScholarPubMed
Dayan, F. E., Weete, J. D., Duke, S. O., and Hancock, H. G. 1997c. Soybean (Glycine max) cultivar differences in response to sulfentrazone. Weed Sci 45:634–641.Google Scholar
Dayan, F. E., Weete, J. D., and Hancock, H. G. 1996. Physiological basis for differential sensitivity to sulfentrazone by sicklepod (Senna obtusifolia) and coffee senna (Cassia occidentalis). Weed Sci 44:12–17.CrossRefGoogle Scholar
Frear, D. S., Swanson, H. R., and Mansager, E. R. 1983. Acifluorfen metabolism in soybean: diphenylether bond cleavage and the formation of homoglutathione, cysteine and glucose conjugates. Pestic. Biochem. Physiol 20:299–310.Google Scholar
Jacobs, J. M., Jacobs, N. J., and Duke, S. O. 1996. Protoporphyrinogen destruction by plant extracts and correlation with tolerance to protoporphyrinogen oxidase-inhibiting herbicides. Pestic. Biochem. Physiol 55:77–83.Google Scholar
Kataoka, M., Sato, R., and Oshio, H. 1990. Isolation and partial characterization of mutant Chlamydomonas reinhardtii resistant to herbicide S-23142. J. Pestic. Sci 15:449–451.Google Scholar
Kenyon, W. H., Duke, S. O., and Vaughn, K. C. 1985. Sequences of effects of acifluorfen on physiological and ultrastructural parameters in cucumber cotyledon discs. Pestic. Biochem. Physiol 24:240–250.Google Scholar
Lermontova, I. and Grimm, B. 2000. Overexpression of plastidic IX oxidase leads to resistance to the diphenyl-ether herbicide acifluorfen. Plant Physiol 122:75–84.Google Scholar
Li, J., Smeda, R. J., and Dayan, F. E. 2003. Two Missouri common waterhemp (Amaranthus rudis) biotypes resistant to protoporphyrinogen oxidase herbicides. Proc. Weed Sci. Soc. Am 43:32.Google Scholar
Matringe, M., Camadro, J-M., Labbe, P., and Scalla, R. 1989a. Protoporphyrinogen oxidase as a molecular target for diphenyl ether herbicides. Biochem. J 260:231–235.Google Scholar
Matringe, M., Camadro, J-M., Labbe, P., and Scalla, R. 1989b. Protoporphyrinogen oxidase inhibition by three peroxidizing herbicides: oxadiazon, LS 82–556 and M&B 39279. FEBS Lett 245:35–38.Google Scholar
Matringe, M. and Scalla, R. 1988. Effects of acifluorfen-methyl on cucumber cotyledons: porphyrin accumulation. Pestic. Biochem. Physiol 32:164–172.Google Scholar
Matringe, M. and Scalla, R. 1992. Characterization of [3H]acifluorfen binding to purified pea etioplasts, and evidence that protoporphyrinogen oxidase specifically binds acifluorfen. Eur. J. Biochem 209:861–868.CrossRefGoogle ScholarPubMed
Matsumoto, H., Lee, J. J., and Ishizuka, K. 1995. Variation in crop response to protoporphyrinogen oxidase inhibitors. Pages 120–132 in Duke, S. O. and Rebeiz, C. A. eds. Porphyric Pesticides: Chemistry, Toxicology, and Pharmaceutical Applications. American Chemical Society Symposium Series 559. Washington, DC: American Chemical Society.Google Scholar
Maxwell, B. D. and Mortimer, A. M. 1994. Selecting for herbicide resistance. Pages 1–25 in Powles, S. B. and Holtum, J.A.M. eds. Herbicide Resistance in Plants, Biology and Biochemistry. Boca Raton: Lewis.Google Scholar
Nandihalli, U. B. and Duke, S. O. 1993. The porphyrin pathway as a herbicide target site. Am. Chem. Soc. Symp. Ser 524:62–78.Google Scholar
Nandihalli, U. B., Sherman, T. D., Duke, M. V., Fisher, J. D., Musco, V. A., Becerril, J. M., and Duke, S. O. 1992. Correlation of protoporphyrinogen oxidase inhibition by O-phenyl pyrrolidino- and piperidino- carbamates with their herbicidal effects. Pestic. Sci 35:227–235.Google Scholar
Randolph-Anderson, B. L., Sato, R., and Johnson, A. M. et al. 1998. Isolation and characterization of a mutant protoporphyrinogen oxidase gene from Chlamydomonas reinhardtii conferring resistance to porphyric herbicides. Plant Mol. Biol 38:839–859.CrossRefGoogle ScholarPubMed
Scalla, R., Matringe, M., Camadro, J-M., and Labbe, P. 1990. Advances in the mode of action of diphenyl ether and related herbicides. Z. Naturforsch 45c:503–511.CrossRefGoogle Scholar
Sherman, T. D., Becerril, J. M., Matsumoto, H., Duke, M. V., Jacobs, J. M., Jacobs, N. J., and Duke, S. O. 1991. Physiological basis for differential sensitivities of plant species to protoporphyrinogen oxidase-inhibiting herbicides. Plant Physiol 97:280–287.Google Scholar
Shoup, D. E., Al-Khatib, K., and Peterson, D. E. 2003. Common waterhemp (Amaranthus rudis) resistance to protoporphyrinogen oxidase- inhibiting herbicides. Weed Sci 51:145–150.Google Scholar
Tischer, W. and Strotmann, H. 1977. Relationship between inhibitor binding by chloroplasts and inhibition of photosynthetic electron transport. Biochim. Biophys. Acta 460:113–125.Google Scholar
Warabi, E., Usui, K., Tanaka, Y., and Matsumoto, H. 2001. Resistance of a soybean cell line to oxyfluorfen by overproduction of mitochondrial protoporphyrinogen oxidase. Pest Manag. Sci 57:743–748.Google Scholar
Warabi, E., Usui, K., and Matsumoto, H. 2002. Differential accumulation of protoporphyrin IX between oxyfluorfen resistant and non-resistant soybean cell lines. Nippon Noyaku Gakkaishi 27:106–110.Google Scholar