Hostname: page-component-77c89778f8-9q27g Total loading time: 0 Render date: 2024-07-17T11:57:54.466Z Has data issue: false hasContentIssue false
  • English
  • Français

Nucleic Acid and Protein Changes Induced by Auxin-Like Herbicides

Published online by Cambridge University Press:  12 June 2017

L. G. Chen ,
C. M. Switzer  and
R. A. Fletcher
L. G. Chen
Affiliation:
Dep. of Environ. Biol., Univ. of Guelph, Guelph, Ontario, Canada
C. M. Switzer
Affiliation:
Dep. of Environ. Biol., Univ. of Guelph, Guelph, Ontario, Canada
R. A. Fletcher
Affiliation:
Dep. of Environ. Biol., Univ. of Guelph, Guelph, Ontario, Canada
Get access Rights & Permissions [Opens in a new window]

Abstract

Cucumber (Cucumus sativus L. ‘Chicago pickling’) and wheat (Triticum aestivum L. ‘Manitou’) seeds were germinated in various concentrations of (2,4-dichlorophenoxy) acetic acid (2,4-D), (2,4,5-trichlorophenoxy)acetic acid (2,4,5-T), 3,6-dichloro-o-anisic acid (dicamba), and 4-amino-3,5,6-trichloropicolinic acid (picloram). The nucleic acid and protein levels in the roots of the two species were determined 4 days after germination and compared on a per root basis. All four herbicides increased the DNA and protein of both cucumber and wheat roots with the increase in cucumber being higher than in wheat. The greatest difference between the tolerant wheat and susceptible cucumber was the effect on the RNA levels. As the concentration of all four herbicides increased, a progressive decrease of RNA levels occurred in wheat. In contrast, in cucumber the herbicides at high concentrations (10 and 100 ppmw) increased the RNA levels by more than 200% over the water controls. When the protein levels in both species were compared on a per unit RNA basis, there was an inverse relationship. In wheat the protein/RNA ratio was higher than the control whereas in cucumber the ratio was lower. A differential alteration of RNA species and interference with protein synthesis is suggested as the basis for selectivity.

Type
Research Article
Information
Weed Science , Volume 20 , Issue 1 , January 1972 , pp. 53 - 55
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Fletcher, R. A. 1969. Retardation of leaf senescence by benzyladine in intact bean plants. Planta 89:1–8.CrossRefGoogle Scholar
2. Holm, E. R., O'Brien, T. J., Key, J. L., and Cherry, J. H. 1970. The influence of auxin and ethylene on chromatindirected ribonucleic acid synthesis in soybean. Plant Physiol. 45:41–45.Google Scholar
3. Key, J. L. 1964. Ribonucleic acid and protein synthesis as essential process for cell elongation. Plant Physiol. 39:365–370.CrossRefGoogle ScholarPubMed
4. Key, J. L. and Shannon, J. C. 1964. Enhancement by auxin of ribonucleic acid synthesis in excised soybean hypocotyl tissue. Plant Physiol. 39:360–364.Google Scholar
5. Key, J. L. 1969. Hormones and nucleic acid metabolism. Annu. Rev. Plant Physiol. 20:449–474.Google Scholar
6. Malhotra, S. S. and Hanson, J. B. 1970. Picloram sensitivity and nucleic acids in plants. Weed Sci. 18:1–4.Google Scholar
7. O'Brien, T. J., Jarvis, B. C., Cherry, J. H., and Hanson, J. B. 1968. Enhancement by 2,4-dichlorophenoxyacetic acid of chromatin RNA polymerase in soybean hypocotyl tissue. Biochim. Biophys. Acta 169:35–43.CrossRefGoogle Scholar
8. Silberger, J. and Skoog, F. 1953. Changes induced by indoleaceted acid in nucleic acid contents and growth of tobacco pith tissue. Science 118:443–444.Google Scholar
9. Tester, C. F. and Dure, L. S. 1967. Nucleic acid synthesis during the hormone-stimulated growth of excised oat coleoptiles. Biochemistry 6:2532–2537.Google Scholar