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Melanopsin and calbindin immunoreactivity in the inner retina of humans and marmosets

Published online by Cambridge University Press:  18 June 2019

Ashleigh J. Chandra ,
Sammy C.S. Lee  and
Ulrike Grünert Open the ORCID record for Ulrike Grünert [Opens in a new window]
Ashleigh J. Chandra
Affiliation:
Save Sight Institute, Discipline of Clinical Ophthalmology, Sydney Medical School, The University of Sydney, Sydney, NSW 2000, Australia Australian Research Council Centre of Excellence for Integrative Brain Function, Sydney Node, The University of Sydney, Sydney, NSW 2000, Australia
Sammy C.S. Lee
Affiliation:
Save Sight Institute, Discipline of Clinical Ophthalmology, Sydney Medical School, The University of Sydney, Sydney, NSW 2000, Australia Australian Research Council Centre of Excellence for Integrative Brain Function, Sydney Node, The University of Sydney, Sydney, NSW 2000, Australia
Ulrike Grünert*
Affiliation:
Save Sight Institute, Discipline of Clinical Ophthalmology, Sydney Medical School, The University of Sydney, Sydney, NSW 2000, Australia Australian Research Council Centre of Excellence for Integrative Brain Function, Sydney Node, The University of Sydney, Sydney, NSW 2000, Australia
*
*Address correspondence to: Ulrike Grünert, Email: ulrike.grunert@sydney.edu.au
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Abstract

In primate retina, the calcium-binding protein calbindin is expressed by a variety of neurons including cones, bipolar cells, and amacrine cells but it is not known which type(s) of cell express calbindin in the ganglion cell layer. The present study aimed to identify calbindin-positive cell type(s) in the amacrine and ganglion cell layer of human and marmoset retina using immunohistochemical markers for ganglion cells (RBPMS and melanopsin) and cholinergic amacrine (ChAT) cells. Intracellular injections following immunolabeling was used to reveal the morphology of calbindin-positive cells. In human retina, calbindin-labeled cells in the ganglion cell layer were identified as inner and outer stratifying melanopsin-expressing ganglion cells, and ON ChAT (starburst amacrine) cells. In marmoset, calbindin immunoreactivity in the ganglion cell layer was absent from ganglion cells but present in ON ChAT cells. In the inner nuclear layer of human retina, calbindin was found in melanopsin-expressing displaced ganglion cells and in at least two populations of amacrine cells including about a quarter of the OFF ChAT cells. In marmoset, a very low proportion of OFF ChAT cells was calbindin-positive. These results suggest that in both species there may be two types of OFF ChAT cells. Consistent with previous studies, the ratio of ON to OFF ChAT cells was about 70 to 30 in human and 30 to 70 in marmoset. Our results show that there are species-related differences between different primates with respect to the expression of calbindin.

Keywords

Calcium-binding proteinCholinergic amacrine cellsMelanopsin-expressing ganglion cellsPrimate retina
Type
Research Article
Information
Visual Neuroscience , Volume 36 , 2019 , E009
Copyright
Copyright © Cambridge University Press 2019 

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