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Localization and expression of insulin-like growth factor in the teleost retina

Published online by Cambridge University Press:  02 June 2009

Andreas F. Mack ,
Steven L. Balt  and
Russell D. Fernald
Andreas F. Mack
Affiliation:
Neuroscience Program, Jordan Hall, Stanford University, Stanford
Steven L. Balt
Affiliation:
Neuroscience Program, Jordan Hall, Stanford University, Stanford
Russell D. Fernald
Affiliation:
Neuroscience Program, Jordan Hall, Stanford University, Stanford
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Abstract

Teleost fish retinas continue to add neurons throughout life, and evidence from in vitro experiments have implicated insulin-like growth factors (IGFs) in this process. To discover whether these factors are expressed in vivo, we have examined their expression in the cichlid fish, Haplochromis burtoni. Three lines of evidence show that IGFs are present in the fish retina. An IGF-I specific antibody, sm 1.2, binds preferentially to the retinal outer plexiform layer, in areas of cone photoreceptor synaptic endings. Northern blots of mRNA hybridized with riboprobes from trout IGF-I and IGF-II genes revealed transcripts of ~6.5 and 4.9 kb, respectively. The IGF-I probe detected an additional transcript of 1.2 kb in liver but not in retinal mRNA. In situ hybridization with digoxigenin-labeled riboprobes revealed that the IGF gene product is localized in the cone photoreceptors. These results show that cone photoreceptors are the source of IGFs in the fish retina, consistent with the hypothesis that IGFs play a role in regulating production of new neurons in the teleost retina.

Keywords

Insulin-like growth factorsNeurogenesisIn situ hybridizationfish retina
Type
Research Articles
Information
Visual Neuroscience , Volume 12 , Issue 3 , May 1995 , pp. 457 - 461
Copyright
Copyright © Cambridge University Press 1995

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