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Light-induced modulation of coupling between AII amacrine cells in the rabbit retina

Published online by Cambridge University Press:  02 June 2009

Stewart A. Bloomfield ,
Daiyan Xin  and
Tristan Osborne
Stewart A. Bloomfield
Affiliation:
Department of Ophthalmology, New York University Medical Center, New York Department of Physiology and Neuroscience, New York University Medical Center, New York
Daiyan Xin
Affiliation:
Department of Ophthalmology, New York University Medical Center, New York
Tristan Osborne
Affiliation:
Department of Ophthalmology, New York University Medical Center, New York
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Abstract

The rod-driven, AII amacrine cells in the mammalian retina maintain homologous gap junctions with one another as well as heterologous gap junctions with on-cone bipolar cells. We used background illumination to study whether changes in the adaptational state of the retina affected the permeabilities of these two sets of gap junctions. To access changes in permeability, we injected single AII amacrine cells with the biotinylated tracer, Neurobiotin, and measured the extent of tracer coupling to neighboring AII cells and neighboring cone bipolar cells. We also measured the center-receptive field size of All cells to assess concomitant changes in electrical coupling. Our results indicate that in well dark-adapted retinas, AII cells form relatively small networks averaging 20 amacrine cells and covering about 75 μm. The size of these networks matched closely to the size of AII cell on-center receptive fields. However, over most of their operating range, AII cells formed dramatically larger networks, averaging 326 amacrine cells, which corresponded to an increased receptive-field size. As the retina was light adapted beyond the operating range of the AII cells, they uncoupled to form networks comparable in size to those seen in well dark-adapted retinas. Our results, then, indicate that the adaptational state of the retina has a profound effect on the extent of electrical coupling between AII amacrine cells. Although we observed light-induced changes in the number of tracer-coupled cone bipolar cells, these appeared to be an epiphenomenon of changes in homologous coupling between AII amacrine cells. Therefore, in contrast to the robust changes in AII–AII coupling produced by background illumination, our data provided no evidence of a light-induced modulation of coupling between AII cells and on-cone bipolar cells.

Keywords

RetinaAmacrine cellCone bipolar cellCouplingGap junctions
Type
Research Articles
Information
Visual Neuroscience , Volume 14 , Issue 3 , May 1997 , pp. 565 - 576
Copyright
Copyright © Cambridge University Press 1997

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