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Cycle GMP-activated channels of rod photoreceptors show neither fast nor slow desensitization

Published online by Cambridge University Press:  02 June 2009

Shu-Ichi Watanabe  and
Gary Matthews
Shu-Ichi Watanabe
Affiliation:
Department of Neurobiology and Behavior, The State University of New York, Stony Brook
Gary Matthews
Affiliation:
Department of Neurobiology and Behavior, The State University of New York, Stony Brook
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Abstract

Desensitization of cGMP-activated channels was examined in excised, inside-out patches obtained from rod photoreceptors. Cyclic GMP was applied using a rapid-flow system in which concentration jumps are complete within 10–50 ms. In outer-segment patches containing many channels, the cGMP-dependent conductance reached a steady plateau that was maintained for tens of seconds in the presence of cGMP; thus, there was no indication of slow desensitization. However, rapid desensitization on the scale of milliseconds could not be ruled out because of limited speed of access of cGMP to the inner face of the patch membrane. To test for rapid desensitization, inner-segment patches containing only a single cGMP-activated channel were used. In these one-channel patches, there was no change in activity of the channel with time from its earliest onset after application of cGMP, indicating that rapid desensitization also did not occur.

Keywords

Ion channelsPhotoreceptorsCyclic GMPDesensitizationPhototransduction
Type
Research Articles
Information
Visual Neuroscience , Volume 4 , Issue 5 , May 1990 , pp. 481 - 487
Copyright
Copyright © Cambridge University Press 1990

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References

Brett, R.S., Dilger, J.P., Adams, P.R. & Lancaster, B. (1986). A method for the rapid exchange of solutions bathing excised membrane patches. Biophysical Journal 50, 987–992.CrossRefGoogle ScholarPubMed
Fesenko, E.E., Kollesnikov, S.S. & Lyubarsky, A.L. (1985). Induction by cyclic GMP of cationic conductance in plasma membrane of rod outer segment. Nature 313, 310–313.CrossRefGoogle ScholarPubMed
Hamill, O.P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F.J. (1981). Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflü;gers Archiv 391, 85–100.CrossRefGoogle ScholarPubMed
Huganir, R.L., Delcour, A.H., Grenngard, P. & Hess, G.P. (1986). Phosphorylation of the nicotinic acetylcholine receptor regulates its rate of desensitization. Nature 321, 774–776.CrossRefGoogle ScholarPubMed
Karpen, J.W., Zimmerman, A.L., Stryer, L. & Baylor, D.A. (1988a). Molecular mechanics of the cyclic-GMP-activated channel of retinal rods. Cold Spring Harbor Symposia on Quantitative Biology 53, 325–332.CrossRefGoogle ScholarPubMed
Karpen, J.W., Zimmerman, A.L., Stryer, L. & Baylor, D.A. (1988b). Gating kinetics of the cyclic-GMP-activated channel of retinal rods; flash photolysis and voltage-jump studies. Proceedings of the National Academy of Sciences of the U.S.A. 85, 1287–1291.CrossRefGoogle ScholarPubMed
Matthews, G. (1986). Comparison of the light-sensitive and cyclic GMP-sensitive conductances of the rod photoreceptor: noise characteristics. Journal of Neuroscience 6, 2521–2526.CrossRefGoogle ScholarPubMed
Matthews, G. (1987). Single-channel recordings demonstrate that cGMP opens the light-sensitive ion channel of the rod photoreceptor. Proceedings of the National Academy of Sciences of the U.S.A. 84, 299–302.CrossRefGoogle ScholarPubMed
Matthews, G. & Watanabe, S.-I. (1987). Properties of ion channels closed by light and opened by guanosine 3',5'-cyclic monophosphate in toad retinal rods. Journal of Physiology 389, 691–715.CrossRefGoogle ScholarPubMed
Matthews, G. & Watanabe, S.-I. (1988). Activation of single-ion channels from toad retinal rod inner segments by cyclic GMP: concentration dependence. Journal of Physiology 403, 389–405.CrossRefGoogle ScholarPubMed
Matthews, G. & Watanabe, S.-I. (1989). Cyclic GMP-activated channels from rod photoreceptors show little desensitization. Biophysical Journal 55, 458a.Google Scholar
Mayer, M.L., Vyklickly, L. & Clements, J. (1989). Regulation of NMDA receptor desensitization in mouse hippocampal neurons by glycine. Nature 338, 425–427.CrossRefGoogle ScholarPubMed
Pearce, L.B., Calhoon, R.D., Burns, P.R., Vincent, A. & Goldin, S.M. (1988). Two functionally distinct forms of guanosine cyclic 3',5'-phosphate stimulated cation channels in a bovine rod photoreceptor disk preparation. Biochemistry 27, 4396–4406.CrossRefGoogle Scholar
Stryer, L. (1986). Cyclic GMP cascade of vision. Annual Review of Neuroscience 9, 87–119.CrossRefGoogle ScholarPubMed
Watanabe, S.-I. & Matthews, G. (1988). Regional distribution of cGMP-activated ion channels in the plasma membrane of the rod photoreceptor. Journal of Neuroscience 8, 2334–2337.CrossRefGoogle ScholarPubMed