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ON and OFF activity gradients in the lateral geniculate nucleus of the cat: A combined 14C 2-deoxyglucose and D, L-2-amino-4-phosphonobutyric acid study

Published online by Cambridge University Press:  02 June 2009

G. A. Thurlow
Affiliation:
Departments of Medical Physiology and Psychology, University of Calgary, Calgary
D. B. Bowling
Affiliation:
Departments of Medical Physiology and Psychology, University of Calgary, Calgary
R. M. Cooper
Affiliation:
Departments of Medical Physiology and Psychology, University of Calgary, Calgary

Abstract

Experiments on the lateral geniculate nucleus (LGN) of the cat based on 14C 2-deoxyglucose (2-DG) autoradiography and intraocular injections of 2-amino-4-phosphonobutyric acid (APB) provided evidence for gradients of metabolic activity in the ON and OFF pathways in layer A, but only very weakly, if at all, in layer Al. Alert and freely moving cats were exposed to square-wave gratings over a 45-min period after injection of the 2-DG. When one eye had been treated previously with APB, contralateral layer A showed a clear gradient of 2-DG label indicating that the remaining OFF pathway was most active ventrally in the layer and, by implication, that the ON pathway is normally most active dorsally. No gradient was apparent in layer Al ipsilateral to the APB eye. Control experiments based on binocular injections of tetrodotoxin (TTX) demonstrated that no gradients were present in the baseline activity within the layers. Finally, monocular injections of TTX provided evidence for gradients of nondominant eye activity in layers A and Al that were maximal near the interlaminar zone between layers A and A1 and declined in mirror-symmetric fashion toward the dorsal border of A and the ventral border of A1.

Combined with earlier anatomical studies showing depth-dependent patterns of geniculo-cortical projection, these results indicate that in the cat, as in several other species, the visual input to striate cortex is partly organized around ON and OFF pathways. In addition, the results suggest that a systematic variation of binocular interaction, perhaps related to ocular dominance, exists through the depths of the geniculate layers. Understanding how the ON and OFF pathways, and binocular interactions, are organized in the thalamus may provide insight into the functional merging of these systems in the cortex.

Type
Research Articles
Copyright
Copyright © Cambridge University Press 1993

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References

Blasdel, G.G. & Fitzpatrick, D. (1984). Physiological organization of layer 4 in macaque striate cortex. Journal of Neuroscience 12, 13191334.Google Scholar
Bowling, D.B. & Michael, C.R. (1984). Terminal patterns of single, physiologically characterized optic tract fibers in the cat's lateral geniculate nucleus. Journal of Neuroscience 4, 198216.CrossRefGoogle ScholarPubMed
Bowling, D.B. & Wieniawa-Narkiewicz, E. (1986). The distribution of ON- and OFF-centre X- and Y-like cells in the A layers of the cat's lateral geniculate nucleus. Journal of Physiology 375, 561572.CrossRefGoogle Scholar
Bowling, D.B. & Wieniawa-Narkiewicz, E. (1987). Differences in the amplitude of X-cell responses as a function of depth in layer A of lateral geniculate nucleus in cat. Journal of Physiology 390, 201212.CrossRefGoogle Scholar
Bowling, D.B. (1989). Evidence for sublaminar organization of the cells of origin of the geniculo-striate pathway in the cat: A novel method for cortical deposition of HRP. Brain Research 502, 180186.CrossRefGoogle ScholarPubMed
Bowling, D.B. & Caverhill, J.I. (1989). ON/OFF organization in the cat lateral geniculate nucleus: Sublaminae vs. columns. Journal of Comparative Neurology 283, 161168.CrossRefGoogle ScholarPubMed
Conway, J.L. & Schiller, P.H. (1983). Laminar organization of tree shrew dorsal lateral geniculate nucleus. Journal of Neurophysiology 50, 13301342.CrossRefGoogle ScholarPubMed
Guido, W., Tumosa, N. & Spear, P.D. (1989). Binocular interaction in the cat's dorsal lateral geniculate nucleus. I. Spatial-frequency analysis of responses of X, Y, and W cells to nondominant eye stimulation. Journal of Neurophysiology 62, 526543.CrossRefGoogle Scholar
Guillery, R.W. (1966). A study of Golgi preparations from the dorsal lateral geniculate nucleus of the adult cat. Journal of Comparative Neurology 128, 2150.CrossRefGoogle ScholarPubMed
Horton, J.C. & Sherk, H. (1984). Receptive-field properties in the cat's lateral geniculate nucleus in the absence of ON-center retinal input. Journal of Neuroscience 4, 374380.CrossRefGoogle ScholarPubMed
Humphrey, A.L., Sur, M., Uhlrich, D.J. & Sherman, S.M. (1985). Projection patterns of individual X- and Y-cell axons from the lateral geniculate nucleus to cortical area 17 in the cat. Journal of Comparative Neurology 233, 159189.CrossRefGoogle ScholarPubMed
LeVay, S. & McConnell, S.K. (1982). ON and OFF layers in the lateral geniculate nucleus of the mink. Nature 300, 350351.CrossRefGoogle ScholarPubMed
McConnell, S.K. & LeVay, S. (1986). Anatomical organization of the visual system of the mink (Mustela vison). Journal of Comparative Neurology 250, 109132.CrossRefGoogle Scholar
Muly, E.C. & Fitzpatrick, D. (1992). The morphological basis for binocular and ON/OFF convergence in the tree shrew striate cortex. Journal of Neuroscience 12, 13191334.CrossRefGoogle ScholarPubMed
Norton, T.T., Rager, G. & Kretz, R. (1985). ON and OFF regions in layer IV of striate cortex. Brain Research 327, 319323.CrossRefGoogle ScholarPubMed
Schiller, P.H. & Malpeli, J.G. (1978). Functional specificity of lateral geniculate nucleus laminae of the Rhesus monkey. Journal of Neurophysiology 41, 788797.CrossRefGoogle ScholarPubMed
Schiller, P.H., Sandell, J.H. & Maunsell, J.H.R. (1986). Functions of the ON and OFF channels of the visual system. Nature 322, 824825.CrossRefGoogle ScholarPubMed
Schiller, P.H. (1992). The ON and OFF channels in the visual system. Trends in Neuroscience 15, 8692.CrossRefGoogle ScholarPubMed
Sherk, H. & Horton, J.C. (1984). Receptive-field properties in the cat's area 17 in the absence of ON-center geniculate input. Journal of Neuroscience 4, 381393.CrossRefGoogle ScholarPubMed
Slaughter, M.M. & Miller, R.F. (1981). 2-Amino-4-phosphobutyric acid: A new pharmacological tool for retinal research. Science 211, 182185.CrossRefGoogle Scholar
Sokoloff, L., Reivich, M., Kennedy, C, Des, Rosiers M.H., Patlak, C.S., Pettigrew, K.D., Sakurada, O. & Shinohara, M. (1977). The [14C]deoxyglucose method for the measurement of local cerebral glucose utilization: Theory, procedure, and normal values in the conscious and anesthetized albino rat. Journal ofNeu-rochemistry 28, 897916.Google Scholar
Stryker, M.P. & Zahs, K.R. (1983). ON and OFF sublaminae in the lateral geniculate nucleus of the ferret. Journal of Neuroscience 3, 19431951.CrossRefGoogle ScholarPubMed
Wong-Riley, M. & Riley, D.A. (1983). The effect of impulse blockage on cytochrome oxidase activity in the cat visual system. Brain Research 261, 185193.CrossRefGoogle ScholarPubMed
Xue, J.T., Ramoa, A.S., Carney, T. & Freeman, R.D. (1987). Binocular interaction in the dorsal lateral geniculate nucleus of the cat. Experimental Brain Research 68, 305310.CrossRefGoogle ScholarPubMed
Zahs, K.R. & Stryker, M.P. (1988). Segregation of ON and OFF afferents to ferret visual cortex. Journal of Neurophysiology 59, 14101429.CrossRefGoogle Scholar

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ON and OFF activity gradients in the lateral geniculate nucleus of the cat: A combined 14C 2-deoxyglucose and D, L-2-amino-4-phosphonobutyric acid study
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