Skip to main content Accessibility help
×
Home

Targeting and processing of membrane-anchored YFP fusion proteins to protein storage vacuoles in transgenic tobacco seeds

  • Ka Leung Fung (a1), Yin Fun Yim (a1), Yu Chung Tse (a1), Yansong Miao (a1), Samuel S.M. Sun (a1) and Liwen Jiang (a1)...

Abstract

Seeds that store proteins in protein storage vacuoles are attractive bioreactors for producing and storing large amounts of pharmaceutical proteins. However, foreign proteins expressed in transgenic plants are subjected to the delivery and modification processes present within plant cells. Here, it is demonstrated that unique membrane sequences deliver a yellow fluorescent protein (YFP) to the seed protein storage vacuoles in transgenic tobacco (Nicotiana tabacum L.) plants, where the YFP is then separated from its membrane anchors. This precise targeting and separation is required for the successful delivery of useful proteins to seed protein storage vacuoles for their stable accumulation in transgenic crops.

Copyright

Corresponding author

*Correspondence: Email: ljiang@cuhk.edu.hk

References

Hide All
Bethke, P.C. and Jones, R.L. (2000) Vacuoles and prevacuolar compartments. Current Opinion in Plant Biology 3, 469475.
Bewley, J.D. and Black, M. (1994) Seeds: Physiology of development and germination (2nd edition). New York, Plenum Press.
Hellwig, S., Drossard, J., Twyman, R.M. and Fischer, R. (2004) Plant cell cultures for the production of recombinant proteins. Nature Biotechnology 22, 14151422.
Jauh, G.Y., Phillips, T.E. and Rogers, J.C. (1999) Tonoplast intrinsic protein isoforms as markers for vacuole functions. Plant Cell 11, 18671882.
Jiang, L.W. and Rogers, J.C. (1998) Integral membrane protein sorting to vacuoles in plant cells: evidence for two pathways. Journal of Cell Biology 143, 11831199.
Jiang, L.W., Sun, S.S.M. (2002) Membrane anchors for vacuolar targeting: application in plant bioreactors. Trends in Biotechnology 20, 99102.
Jiang, L.W., Phillips, T.E., Rogers, S.W. and Rogers, J.C. (2000) Biogenesis of the protein storage vacuole crystalloid. Journal of Cell Biology 150, 755769.
Jiang, L.W., Phillips, T.E., Hamm, C.A., Drozdowicz, Y.M., Rea, P.A., Maeshima, M., Rogers, S.W. and Rogers, J.C. (2001) The protein storage vacuole: a unique compound organelle. Journal of Cell Biology 155, 9911002.
Jiang, L.W., Erickson, A. and Rogers, J.C. (2002) Multivesicular bodies: a mechanism to package lytic and storage functions in one organelle?. Trends in Cell Biology 12, 362367.
Li, Y.B., Rogers, S.W., Tse, Y.C., Lo, S.W., Sun, S.S.M., Jauh, G.Y. and Jiang, L.W. (2002) BP-80 and homologs are concentrared on post-Golgi, probable lytic prevacuolar compartments. Plant and Cell Physiology 43, 726742.
Mo, B.X., Tse, Y.C. and Jiang, L.W. (2003) Organelle identification and proteomics in plant cells. Trends in Biotechnology 21, 331332.
Neuhaus, J.M. and Rogers, J.C. (1998) Sorting of proteins to vacuoles in plant cells. Plant Molecular Biology 38, 127144.
Okita, T.W. and Rogers, J.C. (1996) Compartmentation of proteins in the endomembrane system of plant cells. Annual Review Plant Physiology and Plant Molecular Biology 47, 327350.
Séveno, M., Bardor, M., Paccalet, T., Gomord, V., Lerouge, P. and Faye, L. (2004) Glycoprotein sialylation in plants?. Nature Biotechnology 22, 13511352.
Shimada, T., Fuji, K., Tamura, K., Kondo, M., Nishimura, M., Hara-Nishimura, I. (2003) Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana. Proceedings of the National Academy of Sciences, USA 100, 1609516100.
Tse, Y.C., Mo, B.X., Hillmer, S., Zhao, M., Lo, S.W., Robinson, D.G. and Jiang, L.W. (2004) Identification of multivescular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cells. Plant Cell 16, 672693.
Vitale, A. and Hinz, G. (2005) Sorting of proteins to storage vacuole: how many mechanisms?. Trends in Plant Science 10, 316323.
Vitale, A. and Raikhel, N.V. (1999) What do proteins need to reach different vacuoles?. Trends in Plant Science 4, 149155.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed