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Soybean seed coat cup unloading on plants with low-raffinose, low-stachyose seeds

Published online by Cambridge University Press:  01 September 2009

Suzanne M. Kosina ,
Alexander Castillo ,
Steven R. Schnebly  and
Ralph L. Obendorf
Suzanne M. Kosina
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University, Ithaca, NY14853, USA
Alexander Castillo
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University, Ithaca, NY14853, USA
Steven R. Schnebly
Affiliation:
Pioneer Hi-Bred, A DuPont Business, 810 Sugar Grove Ave., Hwy44, Dallas Center, IA50063, USA
Ralph L. Obendorf*
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University, Ithaca, NY14853, USA
*
*Correspondence Fax: +1 607 255 2644 Email: rlo1@cornell.edu
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Abstract

Sucrose, raffinose and stachyose accumulate in soybean [Glycine max L. (Merrill)] embryos during seed maturation. To determine the relationship of plant maternal composition on seed composition, soluble carbohydrates in three 1-cm2 leaf punches at three plant growth stages (R2, R3, R6) and in seed coat cup exudates in planta were analysed at four 30-min intervals on soybean plants (R5) with low-raffinose, low-stachyose (LRS) seeds expressing the mutant stc1 phenotype; low-raffinose, low-stachyose and low-phytin (LRSP1, LRSP2) seeds expressing the mutant mips phenotype; or normal raffinose, stachyose and phytin (CHECK) seeds expressing the Stc1 and Mips phenotype. Leaf sucrose (23.6 μg cm− 2), myo-inositol (9.3 μg cm− 2), d-chiro-inositol (6.7 μg cm− 2), d-ononitol (0.76 μg cm− 2), d-pinitol (50.1 μg cm− 2) and total soluble carbohydrates (107.1 μg cm− 2) were not significantly different between phenotypes. d-chiro-Inositol, myo-inositol, d-pinitol and sucrose were unloaded from soybean seed coat cups in planta at decreasing rates over the four sequential periods of sampling. Unloading rates of sucrose and myo-inositol were highest for LRS, d-pinitol was highest for LRSP2, and d-chiro-inositol was not different between LRS, LRSP1, LRSP2 and CHECK. Free cyclitols were 60% of total soluble carbohydrates in leaves and 20% in seed coat cup exudates. Except for sucrose and d-pinitol, seed phenotype had little influence on the composition of compounds unloaded from seed coats to maturing embryos of low-raffinose, low-stachyose seeds. Maternally supplied cyclitols may contribute, in part, to changes in the composition of cyclitol galactosides stored in mature seeds.

Keywords

d-chiro-inositolGlycine max (L.) Merrillmyo-inositold-pinitolseed coat cup unloadingsucrose
Type
Research Article
Information
Seed Science Research , Volume 19 , Issue 3 , September 2009 , pp. 145 - 153
Copyright
Copyright © Cambridge University Press 2009

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References

Chappell, A.S., Scaboo, A.M., Wu, X., Nguyen, H., Pantalone, V.R. and Bilyeu, K.D. (2006) Characterization of the MIPS gene family in Glycine max. Plant Breeding 125, 493–500.CrossRefGoogle Scholar
Chiera, J.M. and Grabau, E.A. (2007) Localization of myo-inositol phosphate synthase (GmMIPS-1) during the early stages of soybean seed development. Journal of Experimental Botany 58, 2261–2268.CrossRefGoogle ScholarPubMed
Chiera, J.M., Streeter, J.G. and Finer, J.J. (2006) Ononitol and pinitol production in transgenic soybean containing the inositol methyl transferase gene from Mesembryanthemum crystallinum. Plant Science 171, 647–654.CrossRefGoogle Scholar
Dittrich, P. and Brandl, A. (1987) Revision of the pathway of d-pinitol formation in Leguminosae. Phytochemistry 26, 1925–1926.CrossRefGoogle Scholar
Fehr, W.R. and Caviness, C.E. (1977) Stages of soybean development. Ames, Special Report 80, Cooperative Extension Service, Iowa State University.Google Scholar
Gomes, C.I., Obendorf, R.L. and Horbowicz, M. (2005) myo-Inositol, d-chiro-inositol, and d-pinitol synthesis, transport, and galactoside formation in soybean explants. Crop Science 45, 1312–1319.CrossRefGoogle Scholar
Heaney, R.P., Weaver, C.M. and Fitzsimmons, M.L. (1991) Soybean phytate content – effect on calcium absorption. American Journal of Clinical Nutrition 53, 745–747.CrossRefGoogle ScholarPubMed
Hegeman, C.E., Good, L.L. and Grabau, E.A. (2001) Expression of d-myo-inositol-3-phosphate synthase in soybean. Implications for phytic acid biosynthesis. Plant Physiology 125, 1941–1948.CrossRefGoogle ScholarPubMed
Hipps, P.P., Sehgal, R.K., Holland, W.H. and Sherman, W.R. (1973) Identification and partial characterization of inositol-NAD+ epimerase and inosose: NAD(P)H reductase from the fat body of the American cockroach, Periplaneta americana. Biochemistry 12, 4705–4712.CrossRefGoogle ScholarPubMed
Hitz, W.D., Carlson, T.J., Kerr, P.S. and Sebastian, S.A. (2002) Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds. Plant Physiology 128, 650–660.CrossRefGoogle ScholarPubMed
Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: dependence on flatulence-producing oligosaccharides and cyclitols – review and survey. Seed Science Research 4, 385–405.CrossRefGoogle Scholar
Horbowicz, M., Brenac, P. and Obendorf, R.L. (1998) Fagopyritol B1, O-α-d-galactopyranosyl-(1 → 2)-d-chiro-inositol, a galactosyl cyclitol in maturing buckwheat seeds associated with desiccation tolerance. Planta 205, 1–11.CrossRefGoogle ScholarPubMed
Loewus, F.A. and Murthy, P.P.N. (2000) myo-Inositol metabolism in plants. Plant Science 150, 1–19.CrossRefGoogle Scholar
Ma, J.M., Horbowicz, M. and Obendorf, R.L. (2005) Cyclitol galactosides in embryos of buckwheat stem-leaf-seed explants fed d-chiro-inositol, myo-inositol, or d-pinitol. Seed Science Research 15, 329–338.CrossRefGoogle Scholar
Meis, S.J., Fehr, W.R. and Schnebly, S.R. (2003) Seed source effect on field emergence of soybean lines with reduced phytate and raffinose saccharides. Crop Science 43, 1336–1339.CrossRefGoogle Scholar
Neus, J.D., Fehr, W.R. and Schnebly, S.R. (2005) Agronomic and seed characteristics of soybean with reduced raffinose and stachyose. Crop Science 45, 589–592.CrossRefGoogle Scholar
Nunes, A.C.S., Vianna, G.R., Cuneo, F., Amaya-Farfán, J., de Capdeville, G., Rech, E.L. and Aragão, F.J.L. (2006) RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content. Planta 224, 125–132.CrossRefGoogle ScholarPubMed
Obendorf, R.L. (1997) Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance (Review Update). Seed Science Research 7, 63–74.CrossRefGoogle Scholar
Obendorf, R.L., Horbowicz, M., Dickerman, A.M., Brenac, P. and Smith, M.E. (1998) Soluble oligosaccharides and galactosyl cyclitols in maturing soybean seeds in planta and in vitro. Crop Science 38, 78–84.CrossRefGoogle Scholar
Obendorf, R.L., Odorcic, S., Ueda, T., Coseo, M.P. and Vassallo, E. (2004) Soybean galactinol synthase forms fagopyritol B1 but not galactopinitols: substrate feeding of isolated embryos and heterologous expression. Seed Science Research 14, 321–333.CrossRefGoogle Scholar
Obendorf, R.L., Sensenig, E.M., Wu, J., Ohashi, M., O'Sullivan, T.E., Kosina, S.M. and Schnebly, S.R. (2008 a) Soluble carbohydrates in mature soybean seed after feeding d-chiro-inositol, myo-inositol, or d-pinitol to stem-leaf-pod explants of low-raffinose, low-stachyose lines. Plant Science 175, 650–655.CrossRefGoogle Scholar
Obendorf, R.L., Zimmerman, A.D., Ortiz, P.A., Taylor, A.G. and Schnebly, S.R. (2008 b) Imbibitional chilling sensitivity and soluble carbohydrate composition of low raffinose, low stachyose soybean seed. Crop Science 48, 2396–2403.CrossRefGoogle Scholar
Obendorf, R.L., Zimmerman, A.D., Zhang, Q., Castillo, A., Kosina, S.M., Bryant, E.G., Sensenig, E.M., Wu, J. and Schnebly, S.R. (2009) Accumulation of soluble carbohydrates during seed development and maturation of low-raffinose, low-stachyose soybean. Crop Science 49, 329–341.CrossRefGoogle Scholar
Pak, Y., Huang, L.C., Lilley, K.J. and Larner, J. (1992) In vivo conversion of [3H]myo-inositol to [3H]chiro-inositol in rat tissues. Journal of Biological Chemistry 267, 16904–16910.CrossRefGoogle ScholarPubMed
Parsons, C.M., Zhang, Y. and Araba, M. (2000) Nutritional evaluation of soybean meals varying in oligosaccharide content. Poultry Science 79, 1127–1131.CrossRefGoogle ScholarPubMed
Peterbauer, T. and Richter, A. (2001) Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Science Research 11, 185–198.Google Scholar
Price, K.R., Lewis, J., Wyatt, G.M. and Fenwick, G.R. (1988) Flatulence – causes, relation to diet and remedies. Die Nahrung 32, 609–623.CrossRefGoogle ScholarPubMed
Raboy, V. (2003) myo-Inositol-1,2,3,4,5,6-hexakisphosphate. Phytochemistry 64, 1033–1043.CrossRefGoogle ScholarPubMed
Rainbird, R.M., Thorne, J.H. and Hardy, R.W.F. (1984) Role of amides, amino acids, and ureides in the nutrition of developing soybean seeds. Plant Physiology 74, 329–334.CrossRefGoogle ScholarPubMed
Sebastian, S.A., Kerr, P.S., Pearlstein, R.W. and Hitz, W.D. (2000) Soybean germplasm with novel genes for improved digestibility. pp. 56–73in Drackley, J.K. (Ed.) Soy in animal nutrition. Savoy, Illinois, Federation of Animal Science Societies.Google Scholar
Streeter, J.G. (2001) Simple partial purification of d-pinitol from soybean leaves. Crop Science 41, 1985–1987.CrossRefGoogle Scholar
Streeter, J.G., Lohnes, D.G. and Fioritto, R.J. (2001) Patterns of pinitol accumulation in soybean plants and relationships to drought tolerance. Plant Cell and Environment 24, 429–438.CrossRefGoogle Scholar
Suarez, F.L., Springfield, J., Furne, J.K., Lohrmann, T.T., Kerr, P.S. and Levitt, M.D. (1999) Gas production in humans ingesting a soybean flour derived from beans naturally low in oligosaccharides. American Journal of Clinical Nutrition 69, 135–139.CrossRefGoogle ScholarPubMed
Sun, T.H., Heimark, D.B., Nguyen, T., Nadler, J.L. and Larner, J. (2002) Both myo-inositol to chiro-inositol epimerase activities and chiro-inositol to myo-inositol ratios are decreased in tissues of GK type 2 diabetic rats compared to Wistar controls. Biochemical and Biophysical Research Communications 293, 1092–1098.CrossRefGoogle ScholarPubMed
Thorne, J.H. and Rainbird, R.M. (1983) An in vivo technique for the study of phloem unloading in seed coats of developing soybean seeds. Plant Physiology 72, 268–271.CrossRefGoogle Scholar
Yoshida, K., Yamaguchi, M., Morinaga, T., Ikeuchi, M., Kinehara, M. and Ashida, H. (2006) Genetic modification of Bacillus subtilis for production of d-chiro-inositol, an investigational drug candidate for treatment of type 2 diabetes and polycystic ovary syndrome. Applied and Environmental Microbiology 72, 1310–1315.CrossRefGoogle ScholarPubMed
Zhou, J.R., Fordyce, E.J., Raboy, V., Dickinson, D.B., Wong, M.S., Burns, R.A. and Erdman, J.W. Jr (1992) Reduction of phytic acid in soybean products improves zinc bioavailability in rats. Journal of Nutrition 122, 2466–2473.CrossRefGoogle ScholarPubMed