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Post-dispersal embryo growth and acquisition of desiccation tolerance in Anemone nemorosa L. seeds

  • Natasha Ali (a1), Robin Probert (a1), Fiona Hay (a1), Hannah Davies (a1) and Wolfgang Stuppy (a1)...


A UK seed conservation collection of Anemone nemorosa L. seeds held at the Millennium Seed Bank (MSB) showed low viability in its first post-storage test. Because achenes of A. nemorosa are naturally dispersed when they are green, we tested the hypothesis that seeds may not be fully desiccation tolerant and storable at the time of natural dispersal, and that a post-harvest treatment could increase the proportion of desiccation-tolerant seeds. Achenes harvested at the point of natural dispersal in late May in 2003 and 2004 were either placed immediately on 1% water agar at 20°C (‘laboratory’ treatment), or placed in nylon sachets and buried in leaf litter among plants growing in the wild (‘field’ treatment). Samples were withdrawn at intervals over a period of 168 d and tested for desiccation tolerance (drying to 0.059 g H2O (g DW)− 1) and longevity (controlled ageing at 60% relative humidity and 45°C). An initial increase, followed by a decline, in the proportion of seeds surviving desiccation and in the longevity of both laboratory- and field-treated samples coincided with the development of embryos from globular to heart- and then torpedo-shaped. Developmental arrest was not required for the acquisition of desiccation tolerance, and continued growth and development of the embryo resulted in a loss of desiccation tolerance, similar to that seen in orthodox seeds upon radicle emergence. Furthermore, while A. nemorosa seeds, like many from the Ranunculaceae family, might be described as having morphological or morphophysiological dormancy, this lack of developmental arrest does not fit with the usual concept of dormancy. The implications of these results for the classification systems of seed-storage behaviour and dormancy, and for the long-term conservation of seeds of A. nemorosa, are discussed.


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Baskin, C.C.andBaskin, J.M. (1998) Seeds: Ecology, biogeography, and evolution of dormancy and germination. San Diego, Academic Press.
Baskin, C.C., Meyer, S.E.andBaskin, J.M. (1995) Two types of morphophysiological dormancy in seeds of two genera (Osmorhiza and Erythronium) with an Arcto-Tertiary distribution pattern. American Journal of Botany 82, 293298.
Baskin, J.M.andBaskin, C.C. (2003) Classification, biogeography, and phylogenetic relationships of seed dormancy. pp. 517544in Smith, R.D.; Dickie, J.B.; Linington, S.H.; Pritchard, H.W.; Probert, R.J. (Eds) Seed conservation: Turning science into practice. Royal Botanic Gardens, Kew.
Berjak, P.andPammenter, N.W. (2001) Seed recalcitrance – current perspectives. South African Journal of Botany 67, 7989.
Berjak, P., Farrant, J.M.andPammenter, N.W. (1989) The basis of recalcitrant seed behaviour. pp. 89108in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.
Bewley, J.D.andBlack, M. (1994) Seeds. Physiology of development and germination (2nd edition). New York, Plenum Press.
Bird, A.E. (2006) Thermal methods and comparative seed longevity in the genus Ranunculus L. PhD thesis, Birkbeck College, University of London.
Davies, H.andProbert, R. (2004) Protocol for comparative seed longevity testing. (accessed 10 July 2007).
Daws, M.I., Cleland, H., Chmielarz, P., Gorian, F., Leprince, O., Mullins, C.E., Thanos, C.A., Vandvik, V.andPritchard, H.W. (2006) Variable desiccation tolerance in Acer pseudoplatanus seeds in relation to developmental conditions: a case of phenotypic recalcitrance? Functional Plant Biology 33, 5966.
Engell, K. (1995) Embryo morphology of the Ranunculaceae. Plant Systematics and Evolution (Suppl. 9), 207216.
Eriksson, O. (1995) Seedling recruitment in deciduous forest herbs: the effects of litter, soil chemistry and seed bank. Flora 190, 6570.
Farrant, J.M., Pammenter, N.W., Berjak, P.andWalters, C. (1997) Subcellular organization and metabolic activity during the development of seeds that attain different levels of desiccation tolerance. Seed Science Research 7, 135144.
Grime, J.P., Mason, G., Curtis, A.V., Rodman, J., Band, S.R., Mowforth, M.A.G., Neal, A.M.andShaw, S. (1981) A comparative study of germination characteristics in a local flora. Journal of Ecology 69, 10171059.
Grime, J.P., Hodgson, J.G.andHunt, R. (1988) Comparative plant ecology: A functional approach to common British species. London, Unwin Hyman.
Hay, F.R.andProbert, R.J. (1995) Seed maturity and the effects of different drying conditions on desiccation tolerance and seed longevity in foxglove (Digitalis purpurea L.). Annals of Botany 76, 639647.
Hay, F.R.andSmith, R.D. (2003) Seed maturity: when to collect seeds from wild plants. pp. 97133in Smith, R.D.; Dickie, J.B.; Linington, S.H.; Pritchard, H.W.; Probert, R.J. (Eds) Seed conservation: Turning science into practice. Royal Botanic Gardens, Kew.
Hay, F., Klin, J.andProbert, R. (2006) Can a post-harvest ripening treatment extend the longevity of Rhododendron L. seeds? Scientia Horticulturae 111, 8083.
Holderegger, R. (1996) Effects of litter removal on the germination of Anemone nemorosa L. Flora 191, 175178.
Holderegger, R., Stehlik, I.andSchellner, J.J. (1998) Estimation of the relative importance of sexual and vegetative reproduction in the clonal woodland herb Anemone nemorosa. Oecologia 117, 105107.
ISTA (International Seed Testing Association) (1985) International rules for seed testing. Rules 1985. Seed Science and Technology 13, 356513.
Martin, A.C. (1946) The comparative internal morphology of seeds. American Midland Naturalist 36, 513660.
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499514.
Shirreffs, D.A. (1985) Anemone nemorosa L. Journal of Ecology 73, 10051020.
Stehlik, I.andHolderegger, R. (2000) Spatial genetic structure and clonal diversity of Anemone nemorosa in late successional deciduous woodlands of Central Europe. Journal of Ecology 88, 424435.
Stevens, P.F. (2005) Angiosperm Phylogeny Website, Version 6, May 2005. (accessed 10 July 2007).
Vertucci, C.W.andFarrant, J.M. (1995) Acquisition and loss of desiccation tolerance. pp. 237271in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.


Post-dispersal embryo growth and acquisition of desiccation tolerance in Anemone nemorosa L. seeds

  • Natasha Ali (a1), Robin Probert (a1), Fiona Hay (a1), Hannah Davies (a1) and Wolfgang Stuppy (a1)...


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