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The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis

Published online by Cambridge University Press:  17 March 2010

Ruth C. Martin ,
Masashi Asahina ,
Po-Pu Liu ,
Jessica R. Kristof ,
Jennifer L. Coppersmith ,
Wioletta E. Pluskota ,
George W. Bassel ,
Natalya A. Goloviznina ,
Theresa T. Nguyen  and
Cristina Martínez-Andújar
...Show all authors
Ruth C. Martin
Affiliation:
USDA-ARS, National Forage Seed Production Research Center, Corvallis, Oregon97331, USA;
Masashi Asahina
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Po-Pu Liu
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Jessica R. Kristof
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Jennifer L. Coppersmith
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Wioletta E. Pluskota
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
George W. Bassel
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Natalya A. Goloviznina
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Theresa T. Nguyen
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Cristina Martínez-Andújar
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
M.B. Arun Kumar
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Piotr Pupel
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Hiroyuki Nonogaki*
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
*
*Correspondence Fax: +1 (541) 737-3479 Email: hiro.nonogaki@oregonstate.edu
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Abstract

MicroRNAs (miRNAs) are involved in developmental programmes of plants, including seed germination and post-germination. Here, we provide evidence that two different miRNA pathways, miR156 and miR172, interact during the post-germination stages in Arabidopsis. Mutant seedlings expressing miR156-resistant SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13), which has silent mutations in the miR156 complementary sequence, over-accumulated SPL13 mRNA and exhibited a delay in seedling development. Microarray analysis indicated that SCHNARCHZAPFEN (SNZ), an AP2-like gene targeted by miR172, was down-regulated in these mutants. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and miRNA gel blot analyses showed that the MIR172 genes were up-regulated in mSPL13 mutants. These results suggest that the miRNA regulation cascades (miR156⊣SPL13 → miR172⊣SNZ) play a critical role during the post-germination developmental stages in Arabidopsis.

Keywords

ArabidopsiscascadesmicroRNAmiR156miR172
Type
Research Article
Information
Seed Science Research , Volume 20 , Issue 2 , June 2010 , pp. 79 - 87
Copyright
Copyright © Cambridge University Press 2010

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