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Interactive effects of temperature, carbon dioxide and watering regime on seed germinability of two genotypes of Arabidopsis thaliana

Published online by Cambridge University Press:  27 February 2019

Mohammad I. Abo Gamar  and
Mirwais M. Qaderi Open the ORCID record for Mirwais M. Qaderi [Opens in a new window]
Mohammad I. Abo Gamar
Affiliation:
Department of Biology, Life Science Centre, Dalhousie University, 1355 Oxford Street, Halifax, Nova Scotia, B3H 4R2, Canada
Mirwais M. Qaderi*
Affiliation:
Department of Biology, Life Science Centre, Dalhousie University, 1355 Oxford Street, Halifax, Nova Scotia, B3H 4R2, Canada Department of Biology, Mount Saint Vincent University, 166 Bedford Highway, Halifax, Nova Scotia, B3M 2J6, Canada
*
Author for correspondence: Mirwais M. Qaderi, Email: mirwais.qaderi@msvu.ca
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Abstract

We examined the combined effects of temperature, carbon dioxide (CO2) and watering regime during seed maturation on subsequent germinability and total phenolics of Arabidopsis thaliana [wild-type (WT) and abi1-1 mutant] seeds. Mature seeds were collected from plants that were grown under lower (22/18°C, 16 h light and 8 h dark) or higher (28/24°C, 16 h light and 8 h dark) temperatures, at ambient (400 μmol mol–1) or elevated (700 μmol mol–1) CO2 concentration, and well-watered or water-stressed. Germinated and non-germinated (viable, rotten and empty) seed percentages, germination rate and total phenolics were determined for both genotypes. Higher maturation temperatures increased seed germination percentage, but decreased germination rate, percentage of rotten and non-germinated viable seeds, and total phenolics. Elevated CO2 increased seed total phenolics. Water stress decreased the percentage of non-germinated viable seeds. Neither of the two latter factors affected other measured parameters. Seeds of the abi1-1 mutant had higher total phenolics. The fate of seeds was mostly affected by higher temperatures and water stress. Also, seeds of the abi1-1 mutant had higher germination rate, empty seed percentage and total phenolics than seeds of the WT genotype. Germination percentage was highest for the WT seeds that matured on the water-stressed plants that were grown under higher temperatures at ambient CO2. It can be concluded that higher temperatures had highest effects on seed germinability and other parameters, and elevated CO2 did not alleviate the negative effects of higher temperatures on seed viability.

Keywords

Arabidopsis thalianacarbon dioxideclimate changephenolic compoundsseed germinationtemperaturewater stress
Type
Research Paper
Information
Seed Science Research , Volume 29 , Issue 1 , March 2019 , pp. 12 - 20
Copyright
Copyright © Cambridge University Press 2019 

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