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Feeding stem–leaf–pod explants of pea (Pisum sativum L.) with d-chiro-inositol or d-pinitol modifies composition of α-d-galactosides in developing seeds

Published online by Cambridge University Press:  15 September 2010

Lesław B. Lahuta ,
Wojciech Święcicki ,
Tomasz Dzik ,
Ryszard J. Górecki  and
Marcin Horbowicz
Lesław B. Lahuta*
Affiliation:
Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A/103A, 10-718Olsztyn, Poland
Wojciech Święcicki
Affiliation:
Institute of Plant Genetics Polish Academy of Science, Strzeszyńska 34, 60-479Poznań, Poland
Tomasz Dzik
Affiliation:
Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A/103A, 10-718Olsztyn, Poland
Ryszard J. Górecki
Affiliation:
Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A/103A, 10-718Olsztyn, Poland
Marcin Horbowicz
Affiliation:
Department of Plant Physiology and Genetics, University of Podlasie, Prusa 12, Siedlce08-110, Poland
*
*Correspondence Email: lahuta@uwm.edu.pl
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Abstract

Feeding stem–leaf–pod explants with d-chiro-inositol and d-pinitol was used as a method to modify α-d-galactosides in developing pea (Pisum sativum) seeds. Four genotypes differing in the composition of raffinose, stachyose and verbascose (raffinose family oligosaccharides or RFOs) in seeds – high RFOs (cv. Tiny), low RFOs (SZD175) and low verbascose (cv. Hubal and cv. Wt 506) – were studied. Although seeds of all examined pea lines were able to take up both d-chiro-inositol and d-pinitol, only d-chiro-inositol was effectively converted into its galactosides: mainly fagopyritol B1 (O-α-d-galactopyranosyl-(1 → 2)-d-chiro-inositol) and fagopyritol B2 (O-α-d-galactopyranosyl-(1 → 6)-O-α-d-galactopyranosyl-(1 → 2)-d-chiro-inositol). In seeds of pea lines naturally containing low levels of verbascose (cv. Hubal) and low RFOs (SZD175), the enhanced accumulation of fagopyritols depressed the RFO level by c. 64 and 20%, respectively. Moreover, in both genotypes, about 25 and 30% of total galactose bound in α-d-galactosides occurred in fagopyritols. d-Pinitol present in the pea seeds was converted into monogalactosides, but their accumulation was several-fold lower than that of fagopyritols and did not significantly influence the accumulation of RFOs. Pea seeds with the composition of soluble carbohydrates modified by feeding with either of the cyclitols were able to complete germination.

Keywords

galactosyl cyclitolsd-chiro-inositolpead-pinitolraffinose oligosaccharidesseeds
Type
Research Article
Information
Seed Science Research , Volume 20 , Issue 4 , December 2010 , pp. 213 - 221
Copyright
Copyright © Cambridge University Press 2010

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References

Blöchl, A., Peterbauer, T. and Richter, A. (2007) Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds. Journal of Plant Physiology 164, 1093–1096.CrossRefGoogle ScholarPubMed
Bock, C., Heather, R. and Fawzy, G. (2009) Down-regulation of galactinol synthesis in oilseed Brassica napus leads to significant reduction of antinutritional oligosaccharides. Botany 87, 597–603.CrossRefGoogle Scholar
Chiera, J.M. and Grabau, E.A. (2007) Localization of myo-inositol phosphate synthase (GmMIPS-1) during early stages of soybean seed development. Journal of Experimental Botany 58, 2261–2268.CrossRefGoogle ScholarPubMed
Chiera, J.M., Streeter, J.G. and Finer, J.J. (2006) Ononitol and pinitol production in transgenic soybean containing the inositol methyl transferase gene from Mesembryanthemum crystallinum. Plant Science 171, 647–654.CrossRefGoogle Scholar
Coon, C.N., Leske, K.L., Akavanichan, O. and Cheng, T.K. (1990) Effect of oligosaccharide-free soybean meal on true metabolizable energy and fiber digestion in adult roosters. Poultry Science 69, 787–793.CrossRefGoogle ScholarPubMed
Dierking, E.C. and Bilyeu, K.D. (2008) Association of a soybean raffinose synthase gene with low raffinose and stachyose seed phenotype. The Plant Genome 1, 135–145.CrossRefGoogle Scholar
Gomes, C.I., Obendorf, R.L. and Horbowicz, M. (2005) myo-Inositol, d-chiro-inositol, and d-pinitol synthesis, transport, and galactoside formation in soybean explants. Crop Science 45, 1312–1319.CrossRefGoogle Scholar
Hitz, W.D., Carlson, T.J., Kerr, P.S. and Sebastian, S.A. (2002) Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds. Plant Physiology 128, 650–660.CrossRefGoogle ScholarPubMed
Hoch, G., Peterbauer, T. and Richter, A. (1999) Purification and characterization of stachyose synthase from lentil (Lens culinaris) seeds: galactopinitol and stachyose synthesis. Archives of Biochemistry and Biophysics 366, 75–81.CrossRefGoogle ScholarPubMed
Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: dependence of flatulence-producing oligosaccharides and cyclitols. Review and survey. Seed Science Research 4, 385–405.CrossRefGoogle Scholar
Horbowicz, M., Brenac, P. and Obendorf, R.L. (1998) Fagopyritol B1, O-α-d-galactopyranosyl-(1 → 2)-d-chiro-inositol, a galactosyl cyclitol in maturing buckwheat seeds associated with desiccation tolerance. Planta 205, 1–11.CrossRefGoogle ScholarPubMed
Karner, U., Peterbauer, T., Raboy, V., Jones, D.A., Hedley, J.C. and Richter, A. (2004) myo-Inositol and sucrose concentrations affect the accumulation of raffinose family oligosaccharides in seeds. Journal of Experimental Botany 55, 1981–1987.CrossRefGoogle ScholarPubMed
Klepek, Y.-S., Geiger, D., Stadler, R., Klebl, F., Landouar-Arsivaud, L., Lemoine, R., Hedrich, R. andSauer, N. (2005) Arabidopsis POLYOL TRANSPORTER5, a new member of the monosaccharide transporter-like superfamily, mediates H+-symport of numerous substrates, including myo-inositol, glycerol, and ribose. The Plant Cell 17, 204–218.CrossRefGoogle ScholarPubMed
Lahuta, L.B. (2006) Biosynthesis of raffinose family oligosaccharides and galactosyl pinitols in developing and maturing seeds of winter vetch (Vicia villosa Roth.). Acta Societatis Botanicorum Poloniae 75, 219–227.CrossRefGoogle Scholar
Lahuta, L.B. and Goszczyńska, J. (2009) Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch (Vicia villosa Roth.) seeds. Acta Societatis Botanicorum Poloniae 78, 203–208.CrossRefGoogle Scholar
Lahuta, L.B., Horbowicz, M., Gojło, E., Goszczyńska, J. and Górecki, R.J. (2005a) Exogenously applied d-pinitol and d-chiro-inositol modifies the accumulation of α-d-galactosides in developing tiny vetch (Vicia hirsuta [L.] S.F. Gray) seeds. Acta Societatis Botanicorum Poloniae 74, 287–296.CrossRefGoogle Scholar
Lahuta, L.B., Górecki, R.J. and Horbowicz, M. (2005b) High concentrations of d-pinitol or d-chiro-inositol inhibit the biosynthesis of raffinose family oligosaccharides in maturing smooth tare (Vicia tetrasperma [L.] Schreb.) seeds. Acta Physiologiae Plantarum 27, 505–513.CrossRefGoogle Scholar
Lahuta, L.B., Goszczyńska, J., Horbowicz, M., Holdynski, C. and Górecki, R.J. (2010) Cyclitols affect accumulation of α-d-galactosides in developing Vicia seeds. Acta Physiologiae Plantarum 32, 933–942.CrossRefGoogle Scholar
Obendorf, R.L. (1997) Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance. Seed Science Research 7, 63–74.CrossRefGoogle Scholar
Obendorf, R.L., Odorcic, S., Ueda, T., Coseo, M.P. and Vasallo, E. (2004) Soybean galactinol synthase forms fagopyritol B1 but not galactopinitols, substrate feeding of isolated embryos and heterologous expression. Seed Science Research 14, 321–333.CrossRefGoogle Scholar
Obendorf, R.L., Sensenig, E.M., Wu, J., Ohashi, M., O'Sullivan, T.E., Kosina, S.M. and Schnebly, S.R. (2008) Soluble carbohydrates in mature soybean seed after feeding d-chiro-inositol, myo-inositol, or d-pinitol to stem–leaf–pod explants of low-raffinose, low-stachyose lines. Plant Science 175, 650–655.CrossRefGoogle Scholar
Peterbauer, T. and Richter, A. (2001) Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Science Research 11, 185–197.Google Scholar
Peterbauer, T., Lahuta, L.B., Blöchl, A., Mucha, J., Hedley, C.L., Górecki, R.J. and Richter, A. (2001) Analysis of the raffinose family oligosaccharide pathway in pea seeds with contrasting carbohydrate composition. Plant Physiology 127, 1764–1772.CrossRefGoogle ScholarPubMed
Peterbauer, T., Mach, L., Mucha, J. and Richter, A. (2002a) Functional expression of cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis. Planta 215, 839–846.CrossRefGoogle ScholarPubMed
Peterbauer, T., Mucha, J., Mach, L. and Richter, A. (2002b) Chain elongation of raffinose in pea seeds. Isolation, characterization, and molecular cloning of a multifunctional enzyme catalyzing the synthesis of stachyose and verbascose. Journal of Biological Chemistry 277, 194–200.CrossRefGoogle ScholarPubMed
Peterbauer, T., Karner, U., Mucha, J., Mach, L., Jones, A.D., Hedley, C.L. and Richter, A. (2003) Enzymatic control of the accumulation of verbascose in pea seeds. Plant, Cell and Environment 26, 1385–1391.CrossRefGoogle Scholar
Polowick, P.L., Baliski, D.S., Bock, C., Heather, R. and Fawzy, G. (2009) Over-expression of α-galactosidase in pea seeds to reduce raffinose oligosaccharide content. Botany 87, 526–532.CrossRefGoogle Scholar
Schneider, S., Schneidereit, A., Udvardi, P., Hammes, U., Gramann, M., Dietrich, P. and Sauer, N. (2007) Arabidopsis thaliana INOSITOL TRANSPORTER2 mediates high affinity H+-symport of different inositols across the plasma membrane. Plant Physiology 145, 1395–1407.CrossRefGoogle Scholar
Szczeciński, P., Gryff-Keller, A., Horbowicz, M. and Lahuta, L.B. (2000) Galactosylpinitols isolated from vetch (Vicia villosa Roth.) seeds. Journal of Agricultural and Food Chemistry 48, 2717–2720.CrossRefGoogle ScholarPubMed
Tegeder, M., Wang, X-D., Frommer, W.B., Offler, C.E. and Patrick, J.W. (1999) Sucrose transport into developing seeds of Pisum sativum L. The Plant Journal 18, 151–161.CrossRefGoogle ScholarPubMed
Ueda, T., Coseo, M.P., Harrell, T.J. and Obendorf, R.L. (2005) A multifunctional galactinol synthase catalyzes the synthesis of fagopyritol A1 and fagopyritol B1 in buckwheat seed. Plant Science 168, 681–690.CrossRefGoogle Scholar
Weber, H., Borisjuk, L., Heim, U., Sauer, N. and Wobus, U. (1997) A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in Fava bean seeds. The Plant Cell 9, 895–908.CrossRefGoogle Scholar
Weber, H., Borisjuk, L. and Wobus, U. (2005) Molecular physiology of legume seed development. Annual Review of Plant Biology 56, 253–279.CrossRefGoogle ScholarPubMed
Yasui, T. and Ohashi, H. (1990) The low molecular weight carbohydrate composition of seeds in the Leguminosae – a new taxonomic character in the family. Science Reports of the Tohoku University, Fourth Series, Biology 39, 257–393.Google Scholar
Zhou, J., Chan, K., Wang, T.L., Hedley, C.L., Offler, C.E. and Patrick, J.W. (2009) Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons. Journal of Experimental Botany 60, 71–85.CrossRefGoogle ScholarPubMed