Skip to main content Accessibility help
×
Home

Differential accumulation of abscisic acid and its catabolites in drought-sensitive and drought-tolerant sunflower seeds

  • Andrea Andrade (a1), Ana Vigliocco (a1), Sergio Alemano (a1), Daniel Alvarez (a2) and Guillermina Abdala (a1)...

Abstract

Eleven sunflower (Helianthus annuus L.) inbred lines were evaluated in field and laboratory studies under drought and irrigation. In the field, lines B59, R419 and B67 had reduced seed and oil yield under drought, while no reduction was observed for R432, HAR4 and B71. Lines HA89, R415, R049, RHA274 and R423 presented intermediate responses. In laboratory tests, seeds of line B59 had reduced germination percentages at 200 and 400 mM mannitol, while germination of seeds of lines R432, B71, HAR4, RHA274 and HA89 was reduced only at 400 mM mannitol. Drought-sensitive B59 and drought-tolerant B71 grown under irrigation and drought conditions in the field were selected for hormone assays. Abscisic acid (ABA) and its catabolites in pericarp, embryonic axis and cotyledons of dry and germinated seeds of B59 and B71 were determined. ABA was the major component of the pericarp of dry seeds from B71 and B59 plants grown under drought. The embryonic axis of B71 dry seeds from drought-grown plants also showed high ABA content. The major findings from this study are: (1) the drought-sensitive and -tolerant lines exhibited different ABA and catabolite profiles; (2) water environment during maternal plant growth affected ABA content and the composition of catabolites in mature and germinated seeds; (3) ABA content did not affect germination performance in our conditions; and (4) the dry and germinated seed parts showed different ABA and catabolite profiles.

Copyright

Corresponding author

*Correspondence Fax: +54-358-4676230 E-mail: aandrade@exa.unrc.edu.ar

References

Hide All
Ali-Rachedi, S., Bouinot, D., Wagner, M.-H., Bonnet, M., Sotta, B., Grappin, P. and Jullien, M. (2004) Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana. Planta 219, 479488.
Amzallag, G.N., Nachmias, A. and Lerner, H.R. (1998) Influence of the mode of salinization on reproductive traits of field-grown progeny in Sorghum bicolor. Israel Journal of Plant Science 46, 916.
Andrade, A., Masciarelli, O., Alemano, S., Luna, V. and Abdala, G. (2008) Tomato mutants sensitive to abiotic stress display different ABA levels and metabolism during germination. Seed Science Research 18, 249258.
Baldini, M. and Vannozzi, G.P. (1996) Crop management practice and environmental affects on hullability in sunflower hybrids. Helia 19, 4762.
Baldini, M. and Vannozzi, G.P. (1999) Yield relationship under drought in sunflower genotypes obtained from a wild population and from cultivated sunflowers in rain-out shelter in large pots and field experiments. Helia 22, 8196.
Balsevich, J.J., Cutler, A.J., Lamb, N., Friesen, L.J., Kurz, E.U., Perras, M.R. and Abrams, S.R. (1994) Response of cultured maize cells to (+)-abscisic acid, ( − )-abscisic acid and their metabolites. Plant Physiology 106, 135142.
Benech Arnold, R.L., Fenner, M. and Edwards, P.J. (1991) Changes in germinability, ABA content and ABA embryonic sensitivity in developing seeds of Sorghum bicolor (L.) Moench. induced by water stress during grain filling. New Phytologist 118, 339347.
Casadebaig, P., Debaeke, P. and Lecoeur, J. (2008) Thresholds for leaf expansion and transpiration response to water deficit in a range of sunflower genotypes. European Journal of Agronomy 28, 646654.
Chen, S., Li, J., Wang, T., Polle, A. and Hüttermann, A. (2002) Osmotic stress and ion-specific affects on xylem abscisic acid and the relevance to salinity tolerance in poplar. Journal of Plant Growth Regulation 21, 224233.
Chiwocha, S., Abrams, S., Ambrose, S., Cutler, A., Loewen, A., Ross, A. and Kermode, A. (2003) A method for profiling classes of plant hormones and their metabolites using liquid chromatography–electrospray ionization tandem mass spectrometry: an analysis of hormone regulation of thermodormancy of lettuce (Lactuca sativa L.) seeds. The Plant Journal 35, 405417.
Chiwocha, S., Cutler, A., Abrams, S.R., Ambrose, S.J., Yang, J., Ross, A.R.S. and Kermode, A.R. (2005) The etr1-2 mutation in Arabidopsis thaliana affects the abscisic acid, auxin, cytokinin and gibberellin metabolic pathways during maintenance of seed dormancy, moist-chilling and germination. The Plant Journal 42, 3548.
Chono, M., Honda, I., Shinoda, S., Kushiro, T., Kamiya, Y., Nambara, E., Kawakami, N., Kaneko, S. and Watanabe, Y. (2006) Fields studies on the regulation of abscisic acid content and germinability during grain development of barley: molecular and chemical analysis of pre-harvest sprouting. Journal of Experimental Botany 57, 24212434.
Connor, D.J. and Hall, A.J. (1997) Sunflower physiology. in Schneiter, A.A.; Seiler, A.A. (Eds) Sunflower technology and production. Agronomy Monograph. Vol. 35. American Society of Agronomy, Madison, WI, USApp. 113182.
Connor, D. and Sadras, V. (1992) Physiology of yield expression in sunflower. Field Crop Research 30, 333389.
Cutler, A. and Krochko, J. (1999) Formation and breakdown of ABA. Trends in Plant Science 4, 472478.
Dennis, L. (2004) Étude génetique de l'aptitude au decorticage dês akénes de tournesol (Helianthus annuus L.). Doctoral thesis, École Nationale Supérieure Agronomique de Rennes, France.
Dietz, K.-J., Sauter, A., Wichert, K., Messdaghi, D. and Hartung, W. (2000) Extracellular β-glucosidase activity in barley involved in the hydrolysis of ABA glucose conjugate in leaves. Journal of Experimental Botany 51, 937944.
Durgbanshi, A., Arbona, V., Pozo, O., Miersch, O., Sancho, J.V. and Gómez-Cadenas, A. (2005) Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography–electrospray tandem mass spectrometry. Journal of Agricultural and Food Chemistry 53, 84378442.
Hall, A.J., Chimenti, C.A., Vilella, F. and Freier, G. (1985) Timing of water stress effects on yield components in sunflower. pp. 131–136 in XI International Sunflower Conference, Mar del Plata, Argentina.
Hermann, K., Meinhard, J., Dobrev, P., Linkies, A., Pesek, B., Heß, B., Macháčková, I., Fischert, U. and Leubner-Metzger, G. (2007) 1-Aminocyclopropane-1-carboxylic acid and abscisic acid during the germination of sugar beet (Beta vulgaris L.) a comparative study of fruit and seeds. Journal of Experimental Botany 58, 30473060.
Huang, D., Wu, W., Abrams, S.R. and Cutler, A.J. (2008) The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and enviromental factors. Journal of Experimental Botany 59, 29913007.
Jacobsen, J.V., Pearce, D.W., Poole, A.T., Pharis, R. and Mander, L.N. (2002) Abscisic acid, phaseic acid and gibberellin contents associated with dormancy and germination in barley. Physiologia Plantarum 115, 428441.
Jiang, F. and Hartung, W. (2007) Long-distance signalling of abscisic acid (ABA): the factors regulating the intensity of the ABA signal. Journal of Experimental Botany 59, 3743.
Kushiro, T., Okamoto, M., Nakabayashi, K., Yamagishi, K., Kitamura, S., Asami, T., Hirai, N., Koshiba, T., Kamiya, Y. and Nambara, E. (2004) The Arabidopsis cytochrome P450 CYP707A encodes ABA 8′-hydroxylases: key enzymes in ABA catabolism. EMBO Journal 23, 16471656.
Lee, K.H., Piao, H.L., Kin, H.-Y., Choi, S.M., Jiang, F., Hartung, W., Hwang, I., Kwak, J.M., Lee, I.-J. and Hwang, I. (2006) Activation of glucosidase via stress-induced polymerization rapidly increase active pools of abscisic acid. Cell 126, 11091120.
Mahajan, S. and Tuteja, N. (2005) Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics 444, 139158.
Marion-Poll, A. and Leung, J. (2006) Abscisic acid synthesis, metabolism and signal transduction. pp. 135in Hedden, P.; Thomas, S.G. (Eds) Plant hormone signaling. Annual plant reviews. Vol. 24. Oxford, UK, Blackwell.
Mercau, J.L., Sadras, V.O., Satorre, E.H., Messina, C., Balbi, C., Uribelarrea, M. and Hall, A.J. (2001) On-farm assessment of regional and seasonal variation in sunflower yield in Argentina. Agricultural Systems 67, 83101.
Millar, A.A., Jacobsen, J.V., Ross, J.J., Helliwell, C.A., Poole, A.T., Scofield, G., Reid, J.B. and Gubler, F. (2006) Seed dormancy and ABA metabolism in Arabidopsis and barley: the role of ABA 8′-hydroxylase. The Plant Journal 45, 942954.
Muriel, J.L. and Downes, R.W. (1974) Effect of periods of moisture stress during various phases of growth of sunflower in the greenhouse. pp. 127–132 in Sixth International Sunflower Conference, Bucharest, Romania.
Mwale, S.S., Hamusimbi, C. and Mwansa, K. (2003) Germination, emergence and growth of sunflower (Helinanthus annuus L.) in response to osmotic seed priming. Seed Science and Technology 31, 199206.
Nambara, E. and Marion-Poll, A. (2005) Abscisic acid biosynthesis and catabolism. Annual Review Plant Physiology and Plant Molecular Biology 56, 165185.
Ni, B.-R. and Bradford, K.J. (1993) Germination and dormancy of abscisic acid- and gibberellin-deficient mutant tomato (Lycopersicum esculentum) seeds. Plant Physiology 101, 607617.
Pedranzani, H., Racagni, G., Alemano, S., Miersch, O., Ramírez, I., Peña-Cortés, H., Taleisnik, E., Machado-Domenech, E. and Abdala, G. (2003) Salt tolerant tomato plants show increased levels of jasmonic acid. Plant Growth Regulation 41, 149158.
Pedranzani, H., Sierra de Grado, R., Vigliocco, A., Miersch, O. and Abdala, G. (2007) Pinus pinaster Ait.: abiotic stress and endogenous jasmonates. Plant Growth Regulation 52, 111116.
Perales, L., Arbona, B., Gómez-Cadenas, A., Cornejo, M.-J. and Sanz, A. (2005) A relationship between tolerance to dehydration of rice lines and ability for ABA synthesis under stress. Plant Physiology and Biochemistry 43, 786792.
Raz, V., Bergevoet, J.H.W. and Koornneef, M. (2001) Sequential steps for developmental arrest in Arabidopsis seeds. Development 128, 243252.
Ren, H., Gao, Z., Chen, L., Wei, K., Liu, J., Fan, Y., Davies, W.J., Jia, W. and Zhang, J. (2007) Dynamic analysis of ABA accumulation in relation to the rate of ABA catabolism in maize tissue under water stress. Journal of Experimental Botany 58, 211219.
Rondanini, D., Mantase, A., Savin, R. and Hall, A. (2006) Response of sunflower yield and grain quality to alternating day/night high temperature regimes during grain filling: effects of timing, duration and intensity of exposure to stress. Field Crop Research 96, 4862.
Sauter, A., Dietz, K.J. and Hartung, W. (2002) A possible stress physiological role of abscisic acid conjugates in root to shoot signalling. Plant and Cell Environmental 25, 223228.
Setha, S., Kondo, S., Hirai, N. and Ohigashi, H. (2005) Quantification of ABA and its metabolites in sweet cherries using deuterium-labeled internal standards. Plant Growth Regulation 45, 183188.
Tahir, M.H.N., Imran, M. and Hussain, M.K. (2002) Evaluation of sunflower (Helianthus annuus L.) inbred lines for drought tolerance. International Journal of Agriculture and Biology 4, 398400.
Toyomasu, T., Yamane, H., Murofushi, N. and Inoue, Y. (1994) Effects of exogenously applied gibberellin and red light on the endogenous levels of abscisic acid in photoblastic lettuce seeds. Plant Cell and Physiology 35, 127129.
Unger, P.W. (1982) Time and frequency of irrigation effects on sunflower production and water use. Soil Science Society of America Journal 46, 10721076.
Vigliocco, A., Alemano, S., Miersch, O., Alvarez, D. and Abdala, G. (2007) Endogenous jasmonates during sunflower germination in seeds from plants grown under different soil moisture content. Seed Science Research 17, 9198.
Zhang, J., Jian, W., Yang, J. and Ismail, A. (2006) Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Research 97, 111119.
Zheng, Y.-Z. and Li, T. (2000) Changes in proline levels and abscisic acid content in tolerant/sensitive cultivars of soybean under osmotic conditions. Soybean Genetics NewsLetter 27[online journal] available athttp://www.soygenetics.org/articles/sgn2000-016.htm (accessed February 2009).
Zhou, R., Squires, T.M., Ambrose, S.J., Abrams, S.R., Ross, A.R.S. and Cutler, A. (2003) Rapid extraction of abscisic acid and its metabolites for liquid chromatography–tandem mass spectrometry. Journal of Chromatography A 1010, 7585.
Zhou, R., Cutler, A., Ambrose, S.J., Galka, M.M., Nelson, K.M., Squires, T.M., Loewen, M.K., Juadhav, A.S., Ross, A.R., Taylor, D.C. and Abrams, S.R. (2004) A new abscisic acid catabolic pathway. Plant Physiology 134, 361369.

Keywords

Related content

Powered by UNSILO

Differential accumulation of abscisic acid and its catabolites in drought-sensitive and drought-tolerant sunflower seeds

  • Andrea Andrade (a1), Ana Vigliocco (a1), Sergio Alemano (a1), Daniel Alvarez (a2) and Guillermina Abdala (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.