Hostname: page-component-76fb5796d-qxdb6 Total loading time: 0 Render date: 2024-04-26T08:50:31.361Z Has data issue: false hasContentIssue false
  • English
  • Français

Decline in a seed-specific abscisic acid-responsive glycine-rich protein (GRPF1) mRNA may reflect the release of seed dormancy in Fagus sylvatica during moist prechilling

Published online by Cambridge University Press:  22 February 2007

Lars Christian Mortensen ,
Dolores Rodríguez ,
Gregorio Nicolás ,
Erik Nymann Eriksen  and
Carlos Nicolás
Lars Christian Mortensen*
Affiliation:
Department of Agricultural Sciences, Section for Horticulture, The Royal Veterinary and Agricultural University, Hoejbakkegaard Allé 21, Taastrup, DK-2630, Denmark
Dolores Rodríguez
Affiliation:
Departamento de Fisiologia Vegetal, Facultad de Biologia, Universidad de Salamanca, Salamanca, E-37007, Spain
Gregorio Nicolás
Affiliation:
Departamento de Fisiologia Vegetal, Facultad de Biologia, Universidad de Salamanca, Salamanca, E-37007, Spain
Erik Nymann Eriksen
Affiliation:
Department of Agricultural Sciences, Section for Horticulture, The Royal Veterinary and Agricultural University, Hoejbakkegaard Allé 21, Taastrup, DK-2630, Denmark
Carlos Nicolás
Affiliation:
Departamento de Fisiologia Vegetal, Facultad de Biologia, Universidad de Salamanca, Salamanca, E-37007, Spain
*
*Correspondence: Fax: +45 35 28 34 78 Email: larschristian@mortensen.mail.dk
Get access Rights & Permissions [Opens in a new window]

Abstract

Dormancy and desiccation tolerance were studied in Fagus sylvatica seeds subsequent to 0–26 weeks of either moist prechilling (5°C) or warm (15°C) pretreatment at a restricted water content of 34% (fresh weight basis), i.e. c. 5% below full imbibition. After the pretreatments, seeds were either fully imbibed at 5 or 15°C, or dried for 7 d to around 13% moisture content and then fully imbibed at 5°C. Most of the dormancy was released after 5 weeks of prechilling, with nearly 70% of the seeds germinating at 15°C, and dormancy was fully released after 11 weeks of prechilling. Most of the seed desiccation tolerance, measured as the final germination percentage for dried seeds, was lost between 11 and 13 weeks of prechilling. A further decline in desiccation tolerance was recorded from 13 to 19 weeks, with a moderate recovery after 26 weeks. Germination was compared with the expression of a previously described abscisic-acid-responsive GRPF1 mRNA for a glycine-rich protein in F. sylvatica. There was a clear decline in accumulation of the transcript in seeds during prechilling, but not during the warm pretreatment. The amount of GRPF1 expression correlated significantly (P < 0.001) with dormancy status.

Keywords

Fagus sylvaticamoist prechillingdormancydesiccation toleranceGRPF1expression
Type
Research Article
Information
Seed Science Research , Volume 14 , Issue 1 , March 2004 , pp. 27 - 34
Copyright
Copyright © Cambridge University Press 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aldridge, C.D. and Probert, R.J. (1992) Effects of partial drying on seed germination in the aquatic grasses Zizania palustris L. and Porteresia coarctata (Roxb.) Tateoka. Seed Science Research 2, 199–205.CrossRefGoogle Scholar
Alvarado, V., Nonogaki, H. and Bradford, K.J. (2000) Expression of endo-β-mannanase and SNF-related protein kinase genes in true potato seeds in relation to dormancy, gibberellin and abscisic acid. pp. 347–364. Viémont, J.D.; Crabbé, J. (Eds) Dormancy in plants. Wallingford, CABI Publishing.Google Scholar
Baker, J., Steele, C. and Dure, L. (1988) Sequence and characterization of 6 Lea proteins and their genes from cotton. Plant Molecular Biology 11, 277–291.CrossRefGoogle ScholarPubMed
Bewley, J.D. (1997) Seed germination and dormancy. Plant Cell 9, 1055–1066.CrossRefGoogle ScholarPubMed
Bianco, J., Garello, G. and Le Page-Degivry, M.T. (1994) Release of dormancy in sunflower embryos by dry storage: involvement of gibberellins and abscisic acid. Seed Science Research 4, 57–62.CrossRefGoogle Scholar
Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1992) Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiology 100, 225–230.CrossRefGoogle ScholarPubMed
Bowden-Bonnett, L. and Lord, J.M. (1979) Isolation and cell free translation of total messenger RNA from germinating castor bean endosperm. Plant Physiology 63, 769–773.CrossRefGoogle ScholarPubMed
Bradford, K.J. (1995) Water relations in seed germination. pp. 351–396. in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Close, T.J. (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiologia Plantarum 97, 795–803.CrossRefGoogle Scholar
Debeaujon, I. and Koornneef, M. (2000) Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and embryonic abscisic acid. Plant Physiology 122, 415–424.CrossRefGoogle ScholarPubMed
Delseny, M., Bies-Etheve, N., Carles, C., Hull, G., Vicient, C., Raynal, M., Grellet, F. and Aspart, L. (2001) Late embryogenesis abundant (LEA) protein gene regulation during Arabidopsis seed maturation. Journal of Plant Physiology 158, 419–427.CrossRefGoogle Scholar
Derkx, M.P.M. (2000) Pre-treatment at controlled seed moisture content as an effective means to break dormancy in tree seeds. pp. 69–85. Viémont, J.D.; Crabbé, J. (Eds). Dormancy in plants. Wallingford, CABI Publishing.Google Scholar
Downie, B., Gurusinghe, S. and Bradford, K.J. (1999) Internal anatomy of individual tomato seeds: relationship to abscisic acid and germination physiology. Seed Science Research 9, 117–128.CrossRefGoogle Scholar
Dure, L. (1993) The LEA proteins of higher plants. pp. 325–335. Verma, D.P.S. (Ed.) Control of plant gene expression. Boca Raton, CRC Press.Google Scholar
Dure, L., Crouch, M., Harada, J., Ho, T.h.D., Mundy, J., Quatrano, R., Thomas, T. and Sung, Z.R. (1989) Common amino acid sequence domains among the LEA proteins of higher plants. Plant Molecular Biology 12, 475–486.CrossRefGoogle ScholarPubMed
Finch-Savage, W.E., Pramanik, S.K. and Bewley, J.D. (1994) The expression of dehydrin proteins in desiccation-sensitive (recalcitrant) seeds of temperate trees. Planta 193, 478–485.CrossRefGoogle Scholar
Garello, G. and Le Page-Degivry, M.T. (1999) Evidence for the role of abscisic acid in the genetic and environmental control of dormancy in wheat (Triticum aestivum L.) Seed Science Research 9, 219–226.CrossRefGoogle Scholar
Garello, G., Barthe, P., Bonelli, M., Bianco-Trinchant, J., Bianco, J. and Le Page-Degivry, M.T. (2000) Abscisic acid-regulated responses of dormant and non-dormant embryos of Helianthus annuus: role of ABA-inducible proteins. Plant Physiology and Biochemistry 38, 473–482.CrossRefGoogle Scholar
Grappin, P., Bouinot, D., Sotta, B., Miginiac, E. and Jullien, M. (2000) Control of seed dormancy in Nicotiana plumbaginifolia: post-imbibition abscisic acid synthesis imposes dormancy maintenance. Planta 210, 279–285.CrossRefGoogle ScholarPubMed
Greggains, V., Finch-Savage, W.E., Quick, W.P. and Atherton, N.M. (2000) Putative desiccation tolerance mechanisms in orthodox and recalcitrant seeds of the genus Acer. Seed Science Research 10, 317–327.CrossRefGoogle Scholar
Groot, S.P.C., van Yperen, I.I. and Karssen, C.M. (1991) Strongly reduced levels of endogenous abscisic acid in developing seeds of tomato mutant sitiens do not influence in vivo accumulation of dry matter and storage proteins. Physiologia Plantarum 81, 73–78.CrossRefGoogle Scholar
Hilhorst, H.W.M. (1995) A critical update on seed dormancy. I. Primary dormancy. Seed Science Research 5, 61–73.CrossRefGoogle Scholar
Hilhorst, H.W.M. and Karssen, C.M. (1992) Seed dormancy and germination: the role of abscisic acid and gibberellins and the importance of hormone mutants. Plant Growth Regulation 11, 225–238.CrossRefGoogle Scholar
ISTA (International Seed Testing Association) (1985) International rules for seed testing. Seed Science and Technology 13, 356–513.Google Scholar
Johnson, R.R. and Dyer, W.E. (2000) Degradation of endosperm mRNAs during dry after-ripening of cereal grains. Seed Science Research 10, 233–241.CrossRefGoogle Scholar
Jones, H.D., Peters, N.C.B. and Holdsworth, M.J. (1997) Genotype and environment interact to control dormancy and differential expression of the VIVIPAROUS 1 homologue in embryos of Avena fatua. Plant Journal, 12, 911–920.CrossRefGoogle ScholarPubMed
Jones, S.K. and Gosling, P.G. (1994) ‘Target moisture content’ prechill overcomes the dormancy of temperate conifer seeds. New Forests 8, 309–321.CrossRefGoogle Scholar
Jullien, M. and Bouinot, D. (1997) Seed dormancy and responses of seeds to phytohormones in Nicotiana plumbaginifolia. pp. 203–214. in Ellis, R.H.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, Kluwer Academic.CrossRefGoogle Scholar
Koornneef, M., Alonso-Blanco, C., Bentsink, L., Blankestijn-de, Vries H., Debeaujon, I., Hanhart, C.J., Leon-Kloosterziel, K.M., Peeters, A.J.M. and Raz, V. (2000) The genetics of seed dormancy in Arabidopsis thaliana. pp. 365–373. in Viémont, J.D.; Crabbé, J. (Eds) Dormancy in plants. Wallingford, CABI Publishing.Google Scholar
Le Page-Degivry, M.T., Barthe, P. and Garello, G. (1990) Involvement of endogenous abscisic acid in onset and release of Helianthus annuus embryo dormancy. Plant Physiology 92, 1164–1168.CrossRefGoogle ScholarPubMed
Le Page-Degivry, M.T., Barthe, P., Bianco, J. and Garello, G. (1997) ABA involvement in the psychrolabile dormancy of Fagus embryo. pp. 215–224. in Ellis, R.H.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, Kluwer Academic.CrossRefGoogle Scholar
Lorenzo, O., Rodríguez, D., Nicolás, G., Rodríguez, P.L. and Nicolás, C. (2001) A new protein phosphatase 2C (FsPP2C1) induced by abscisic acid is specifically expressed in dormant beechnut seeds. Plant Physiology 125, 1949–1956.CrossRefGoogle ScholarPubMed
Meinke, D.W., Cherry, J.M., Dean, C., Rounsley, S.D. and Koornneef, M. (1998) Arabidopsis thaliana: a model plant for genome analysis. Science 282, 662–682.CrossRefGoogle Scholar
Morris, C.F., Anderberg, R.J., Goldmark, P.J. and Walker-Simmons, M.K. (1991) Molecular cloning and expression of abscisic-responsive genes in embryos of dormant wheat seeds. Plant Physiology 95, 814–821.CrossRefGoogle ScholarPubMed
Morris, P.C., Kumar, A., Bowles, D.J. and Cuming, A.C. (1990) Osmotic stress and abscisic acid induce expression of the wheat Em genes. European Journal of Biochemistry 190, 625–630.CrossRefGoogle ScholarPubMed
Müller, C. and Bonnet-Masimbert, M. (1989) Breaking dormancy before storage: an improvement to processing of beechnuts (Fagus sylvatica L.) Seed Science and Technology 17, 15–26.Google Scholar
Müller, C., Laroppe, E. and Bonnet-Masimbert, M. (1999) Further development in the redrying and storage of prechilled beechnuts (Fagus sylvatica L.): effect of seed moisture content and prechilling duration. Annals of Forest Science 56, 49–57.CrossRefGoogle Scholar
Nambara, E., Kawaide, H., Kamiya, Y. and Naito, S. (1998) Characterization of an Arabidopsis thaliana mutant has a defect in ABA accumulation: ABA-dependent and ABA-independent accumulation of free amino acids during dehydration. Plant and Cell Physiology 39, 853–858.CrossRefGoogle Scholar
Nicolás, C., Nicolás, G. and Rodríguez, D. (1996) Antagonistic effects of abscisic acid and gibberellic acid on the breaking of dormancy of Fagus sylvatica seeds. Physiologia Plantarum 96, 244–250.CrossRefGoogle Scholar
Nicolás, C., Rodríguez, D., Poulsen, F.V., Eriksen, E.N. and Nicolás, G. (1997) The expression of an abscisic acid-responsive glycine-rich protein coincides with the level of seed dormancy in Fagus sylvatica. Plant and Cell Physiology 38, 1303–1310.CrossRefGoogle ScholarPubMed
Oishi, M.Y. and Bewley, J.D. (1990) Distinction between the responses of developing maize kernels to fluridone and desiccation in relation to germinability, α-amylase activity, and abscisic acid content. Plant Physiology 94, 592–598.CrossRefGoogle ScholarPubMed
Ooms, J.J.J., Leon-Kloosterziel, K.M., Bartels, D., Koornneef, M. and rssen, C.M. (1993) Acquisition of desiccation tolereance and longevity in seeds of Arabidopsis thaliana – a comparative study using abscisic acid-insensitive abi3 mutants. Plant Physiology 102, 1185–1191.CrossRefGoogle ScholarPubMed
Ranford, J.C., Bryce, J.H. and Morris, P.C. (2002) PM19, a barley (Hordeum vulgare L.) gene encoding a putative plasma membrane protein, is expressed during embryo development and dormancy. Journal of Experimental Botany 53, 147–148.Google ScholarPubMed
Sachetto-Martins, G., Franco, L.O. and de Oliveira, D.E. (2000) Plant glycine-rich proteins: a family or just proteins with a common motif? Biochimica et Biophysica Acta 1492, 1–14.CrossRefGoogle ScholarPubMed
Suszka, B. (1975) Cold storage of already after-ripened beech (Fagus sylvatica L.) seeds. Arboretum Kornickie 20, 299–315.Google Scholar
Thomsen, K.A. (1997) The effect of harvest time and drying on dormancy and storability in beechnuts. pp. 45–51. in Ellis, R.H.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, Kluwer Academic.CrossRefGoogle Scholar
Vertucci, C.W. and Farrant, J.M. (1995) Acquisition and loss of desiccation tolerance. pp. 237–271. in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Wang, M., Heimovaara-Dijkstra, S. and Van Duijn, B. (1995) Modulation of germination of embryos isolated from dormant and nondormant barley grains by manipulation of endogenous abscisic acid. Planta 195, 586–592.CrossRefGoogle Scholar
White, C.N. and Rivin, C.J. (2000) Gibberellins and seed development in maize. II. Gibberellin synthesis inhibition enhances abscisic acid signalling in cultured embryos. Plant Physiology 122, 1089–1097.CrossRefGoogle ScholarPubMed
White, C.N., Proebsting, W.M., Hedden, P. and Rivin, C.J. (2000) Gibberellins and seed development in maize. I. Evidence that gibberellin/abscisic acid balance governs germination versus maturation pathways. Plant Physiology 122, 1081–1088.CrossRefGoogle Scholar
Xia, J.-H. and Kermode, A.R. (1999) Analyses to determine the role of embryo immaturity in dormancy maintenance of yellow cedar (Chamaecyparis nootkatensis) seeds: synthesis and accumulation of storage proteins and proteins implicated in desiccation tolerance. Journal of Experimental Botany 50, 107–118.CrossRefGoogle Scholar