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Cryopreservation of seeds of four coffee species (Coffea arabica, C. costatifructa, C. racemosa and C. sessiliflora): importance of water content and cooling rate

  • Stéhane Dussert (a1), Nathalie Chabrillange (a1), Florent Engelmann (a2), François Anthony (a3), Jacques Louarn (a4) and Serge Hamon (a1)...

Abstract

In the range of water contents studied (0.1–0.4 g H2O g dw−1), Coffea arabica seeds were less sensitive to desiccation than C. costatifructa, C. racemosa and C. sessiliflora seeds. At 0.20 g H2O g dw−1, 53% of C. arabica seeds germinated after direct immersion in LN (rapid cooling, 200°C min−1), but none of them developed into normal seedlings. By contrast, in C. costatifructa, C. racemosa and C. sessiliflora, when seeds were dehydrated to the optimal water content (0.19, 0.28 and 0.31 g H2O g dw−1, respectively), the percentages of seeds which developed into normal seedlings after LN exposure were 26, 78 and 31% of the desiccation control, respectively. Normal seedlings could be recovered from cryopreserved C. arabica seeds only if they were desiccated to 0.20 g H2O g dw−1 and precooled slowly to −50°C prior to immersion in LN. Precooling seeds at 2°C min−1 allowed 25% of seeds to develop into normal seedlings. The thawing rate had no effect on the survival of cryopreserved C. arabica seeds. In all cryopreservation experiments, the total germination did not reflect the percentage of seeds which developed into normal seedlings. Examination of excised embryos indicated a partial explanation of this difference since only the shoot apex was destroyed in abnormal embryos, whereas the hypocotyl and radicle were normal.

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*Correspondence E-mail stephane.dussert@mpl.orstom.fr

References

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Abdelnour-Esquivel, A, Villalobos, V and Engelmann, F (1992) Cryopreservation of zygotic embryos of Coffea spp Cryo-Letters 13, 297302.
Becwar, M R, Stanwood, P C and Lehonardt, K W (1983) Dehydration effects on freezing characteristics and survival in liquid nitrogen of desiccation-tolerant and desiccation-sensitive seeds journal of the American Society for Horticultural Science 108, 613618.
Berthaud, J and Charrier, A (1988) Genetic resources of Coffea. pp 142in Clarke, R J and Macrae, R (Eds) Coffee, Vol. 4, Agronomy. London, Elsevier Applied Science.
Bertrand-Desbrunais, A and Charrier, A (1989) Conservation des ressources génétiques caféières en vitrothèque. pp 438–47 in Proceedings of the 13th ASIC, Paipa, Colombia.
Chaudhury, R and Chandel, K P S (1994) Germination studies and cryopreservation of seeds of black pepper (Piper nigrum L.) – a recalcitrant species Cryo-Letters 15, 145150.
Chin, H F, Krishnapillany, B and Alang, Z C (1988) Cryopreservation of Veitchia and Howea palm embryos: non-development of the haustorium Cryo-Letters 9, 372379.
Couturon, E (1980) Le maintien de la viabilité des graines de caféiers par le contrôle de leur teneur en eau et de la température de stockage Café Cacao Thé 1, 2732.
Dickie, J B, May, K, Morris, S V A and Titley, S E (1991) The effects of desiccation on seed survival in Acer platanoides L. and Acer pseudoplatanus L Seed Science Research 1, 149162.
Dussert, S, Chabrillange, N, Anthony, F, Engelmann, F, Recalt, C and Hamon, S (1997) Variability in storage response within a coffee (Coffea spp.) core collection under slow growth conditions Plant Cell Reports 16, 343348.
Ellis, R H, Hong, T D and Roberts, E H (1990) An intermediate category of seed storage behaviour? I. Coffee, journal of Experimental Botany 41, 11671174.
Ellis, R H, Hong, T D and Roberts, E H (1991) An intermediate category of seed storage behaviour? II. Effects of provenance, immaturity, and imbibition on desiccation-tolerance in coffee Journal of Experimental Botany 42, 653657.
Hu, J, Guo, C G and Shi, S X (1994) Partial drying and post-thaw preconditioning improve the survival and germination of cryopreserved seeds of tea (Camellia sinensis) Plant Genetic Resources Newsletter 98, 2528.
Hong, T D and Ellis, R H (1995) Interspecific variation in seed storage behaviour within two genera – Coffea and Citrus Seed Science and Technology 23, 165181.
Normah, M N and Vengadasalam, M (1992) Effects of moisture content on cryopreservation of Coffea and Vigna seeds and embryos Cryo-Letters 13, 199208.
Normah, M N, Reed, B M and Yu, X (1994) Seed storage and cryoexposure behavior in hazelnut (Corylus avellana L. cv. Barcelona) Cryo-Letters 15, 315322.
Pence, V C (1995) Cryopreservation of recalcitrant seeds. pp 2950in Bajaj, Y P S (Ed.) Cryopreservation of plant germplasm I. Berlin, Springer-Verlag.
Roberts, E H (1973) Predicting the storage life of seeds Seed Science and Technology 1, 499514.
Roos, E E and Stanwood, P C (1981) Effects of low temperature, cooling rate, and moisture content on seed germination of lettuce, Journal of the American Society for Horticultural Science 106, 3034.
Ryan, T A (1960) Significance tests for multiple comparison of proportions, variances and other statistics Psychological Bulletin 57, 318328.
Stanwood, P C (1985) Cryopreservation of seed germplasm for genetic conservation, pp 199226in Kartha, K K (Ed.) Cryopreservation of plant cell and organs. Boca Raton, CRC press.
Stanwood, P C (1987) Survival of sesame seeds at the temperature (–196°C) of liquid nitrogen Crop Science 27, 327331.
Tompsett, P B (1984) Desiccation studies in relation to the storage of Araucaria seed Annals of Applied Biology 105, 581586.
Tompsett, P B (1987) Desiccation and storage studies on Dipterocarpus seeds Annals of Applied Biology 110, 371379.
Van der Vossen, H A M (1977) Methods of preserving the viability of coffee seed in storage Kenya Coffee 45, 3135.
Vertucci, C W (1989) Effects of cooling rate on seeds exposed to liquid nitrogen temperatures Plant Physiology 90, 14781485.

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Cryopreservation of seeds of four coffee species (Coffea arabica, C. costatifructa, C. racemosa and C. sessiliflora): importance of water content and cooling rate

  • Stéhane Dussert (a1), Nathalie Chabrillange (a1), Florent Engelmann (a2), François Anthony (a3), Jacques Louarn (a4) and Serge Hamon (a1)...

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