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Changes in selected plant growth regulators during germination in sorghum

Published online by Cambridge University Press:  19 September 2008

J. Dewar ,
J. R. N. Taylor  and
P. Berjak
J. Dewar*
Affiliation:
CSIR Food Science and Technology, PO Box 395, Pretoria, 0001, South Africa
J. R. N. Taylor
Affiliation:
Department of Food Science, University of Pretoria, Cereal Foods Research Unit, Pretoria, 0002, South Africa
P. Berjak
Affiliation:
Department of Biology, University of Natal, Plant Cell Biology Research Unit, Durban, 4000, South Africa
*
*Correspondence Fax 00 27 12 841 2386 E-mail jdewar@csir.co.za
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Abstract

The technique of radioimmunoassay following sample resolution by HPLC was used to assay the amounts of the cytokinins zeatin (Z), zeatin riboside (ZR) and isopentenyladenine (IPA), the combined amounts of gibberellins1+3 (GA1+3), and the amounts of indole acetic acid (IAA) and abscisic acid (ABA) during germination in grains of sorghum. Concentrations of GA1+3 were low throughout germination and did not appear to be related to the time of germination. In the mature, non-germinated grain, the concentration of each of the other plant growth regulators was much higher in the smaller component comprised of the embryonic axis and scutellum than in the much larger endosperm tissue. During the germination period studied (64 h), these concentrations declined, with a peak in the amount of the cytokinin IPA and a small peak in Z+ZR (24 h) in the embryo following the first visible signs of root protrusion and coincident with a large enhancement in amylase activity. The high concentration of ABA in the embryo tissue prior to germination was noteworthy. It is suggested that the interaction of ABA and the cytokinins IPA and Z+ZR may play a significant role in controlling sorghum germination.

Keywords

germinationmaltingplant growthregulatorssorghum
Type
Physiology and biochemistry
Information
Seed Science Research , Volume 8 , Issue 1 , March 1998 , pp. 1 - 8
Copyright
Copyright © Cambridge University Press 1998

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