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Calcium, with magnesium, is essential for normal seedling development from partially dehydrated recalcitrant axes: a study on Trichilia dregeana Sond.

  • Patricia Berjak (a1) and David Mycock (a2)

Abstract

Assessment of the rehydration procedure has been consistently overlooked in evaluation of factors contributing to successful cryostorage of partially dehydrated, embryonic axes excised from recalcitrant seeds. Conventional rehydration of Trichilia dregeana (Sond.) axes in distilled water resulted in the lack of root pole gravitropism after culture on medium in vitro. In comparison, a strong gravitropic response was observed in axes that had not been dehydrated, and by those rehydrated in a solution containing 1 M CaCl2 and 1 mM MgCl2. However, no marked loss of either cation from the tissue could be detected, whether axes were rehydrated in distilled water or the Ca2+/Mg2+ solution. Starch-packed statoliths differentiated rapidly in both non-dehydrated axes and those rehydrated in the divalent cation solution, but these organelles failed to develop or accumulate much starch following axis rehydration in water, as was the case for the amyloplasts of root cells generally. After rehydration in the Ca2+/Mg2+ solution and 48 h in culture, axis root-cap columella cells accumulated Ca2+, whereas axes rehydrated in water or solutions containing either Ca2+ or Mg2+ alone did not take up Ca2+. Rehydration with the Ca2+/Mg2+ solution also altered distribution of the actin component of the cytoskeleton. When rehydrated in the divalent cation solution, actin was associated with the nucleus and with the statoliths, which were located distally in statocytes of axes. In contrast, actin was largely confined to the perinuclear area in root-cap columella cells of the agravitropic, water-rehydrated axes. The present results indicate a definitive primary role for Ca2+ with Mg2+ in graviperception, via starch metabolism, and in the determination of statolith morphology, which appears to be linked with maintenance of the actin component of the cytoskeleton in root-cap statocytes.

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Corresponding author

*Correspondenceberjak@ukzn.ac.za
§Formerly University of Natal.

References

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Baluška, F. and Hasenstein, K.H. (1997) Root cytoskeleton: its role in perception and response to gravity. Planta 203, (suppl.), S69S78.
Baluška, F., Polsakiewicz, M., Peters, M. and Volkmann, D. (2000) Tissue specific subcellular immunolocalization of myosin-like protein in maize root apices. Protoplasma 212, 137145.
Barlow, P.W. (2003) The root cap: Cell dynamics, cell differentiation and cap function. Journal of Plant Growth Regulation 21, 261286.
Belyavskaya, N.A. (1996) Calcium and graviperception in plants: inhibitor analysis. International Review of Cytology 168, 123185.
Berjak, P. and Pammenter, N.W. (2001) Seed recalcitrance – current perspectives. South African Journal of Botany 67, 7989.
Berjak, P. and Pammenter, N.W. (2004) Recalcitrant seeds. in Benech-Arnold, R.L.;, Sánchez, R.A. (Eds) Seed physiology: Applications to agriculture. New York, Haworth Press (in press).
Berjak, P., Mycock, D.J., Wesley-Smith, J., Dumet, D. and Watt, P. (1996) Strategies for in vitro conservation of hydrated germplasm. pp. 1952in Normah, M.N.;, Narimah, M.K.;, Clyde, M.M. (Eds) In vitro conservation of plant genetic resources. Kuala Lumpur, Malaysia, Percetakan Watan Sdn. Bhd.
Berjak, P., Walker, M., Watt, M.P. and Mycock, D.J. (1999) Experimental parameters underlying failure or success in plant germplasm cryopreservation: a case study on zygotic axes of Quercus robur L. CryoLetters 20, 251262.
Berjak, P., Mycock, D.J., Walker, M., Kioko, J.I., Pammenter, N.W. and Wesley-Smith, J. (2000) Conservation of genetic resources naturally occurring as recalcitrant seeds. pp. 223228in Black, M.;, Bradford, K.J.;, Vásquez-Ramos, J.; (Eds) Seed biology: Advances and applications. Wallingford, CABI Publishing.
Blancaflor, E.B. and Hasenstein, K.H. (1993) Organization of cortical microtubules in graviresponding maize roots. Planta 191, 231237.
Böcher, M., Erdmann, H., Heim, S. and Wylegalla, C. (1985) Protein kinase activity of in vitro cultured plant cells in relation to growth and starch metabolism. Journal of Plant Physiology 119, 209218.
Busch, M.B. and Sievers, A. (1990) Hormone treatment of roots causes not only a reversible loss of starch, but also of structural polarity in statocytes. Planta 181, 358364.
Busch, M.B., Kortje, K.H., Rahmann, H. and Sievers, A. (1993) Characteristic and differential calcium signals from cell structures of the root cap detected by energy-filtering electron microscopy (EELS/ESI). European Journal of Cell Biology 60, 88100.
Chandra, S., Chabot, J.F., Morrison, G.H. and Leopold, A.C. (1982) Localization of calcium in amyloplasts of root-cap cells using ion microscopy. Science 216, 12211223.
Chang, S-C., Cho, M.H., Kang, B.G. and Kaufman, P.B. (2001) Changes in starch content in oat (Avena sativa) shoot pulvini during the gravitropic response. Journal of Experimental Botany 52, 10291040.
Chang, S.C., Cho, M.H., Kim, S-K., Lee, J.S., Kirakosyan, A. and Kaufman, P.B. (2003) Changes in phosphorylation of 50 and 53 kDa soluble proteins in graviresponding oat (Avena sativa) shoots. Journal of Experimental Botany 54, 10131022.
Collings, D.A., Zsuppan, G., Allen, N.S. and Blancaflor, E.B. (2001) Demonstration of prominent actin filaments in the root columella. Planta 212, 392403.
Dauwalder, M., Roux, S.J. and Rabenberg, L.K. (1985) Cellular and subcellular localization of calcium in gravistimulated corn roots. Protoplasma 129, 137148.
Dauwalder, M., Roux, S.J. and Hardison, L. (1986) Distribution of calmodulin in pea seedlings: Immunocytochemical localization in plumules and root apices. Planta 168, 461470.
Driss-Ecole, D., Lefranc, A. and Perbal, G. (2003) A polarized cell: the root statocyte. Physiologia Plantarum 118, 305312.
Evans, M.L. (1991) Gravitropism: interaction of sensitivity modulation and effector redistribution. Plant Physiology 95, 15.
Gibbon, B.C. and Staiger, C.J. (2000) Profilin. pp. 4565in Staiger, C.J.;, Baluška, F.;, Volkmann, D.;, Barlow, P. (Eds) Actin: A dynamic framework for multiple plant cell functions. Dordrecht, Kluwer Publishers.
Goveia, M.G.T., Kioko, J.I. and Berjak, P. (2004) Developmental status is a critical factor in the selection of excised recalcitrant axes as explants for cryopreservation: a study on Trichilia dregeana Sond. Seed Science Research 14, 241248.
Iversen, T.H. (1969) Elimination of geotropic responsiveness in roots of cress (Lepidium sativum) by removal of statolith starch. Physiologia Plantarum 22, 12511262.
Kioko, J.I. (2003) Aspects of post-harvest seed physiology and cryopreservation of the germplasm of three medicinal plant species indigenous to Kenya and South Africa. PhD Thesis, University of Natal, Durban, South Africa.
Kioko, J., Berjak, P., Pammenter, N.W., Watt, M.P. and Wesley-Smith, J. (1998) Desiccation and cryopreservation of embryonic axes of Trichilia dregeana Sond. CryoLetters 19, 514.
Kovar, D.R., Drøbak, B.K. and Staiger, C.J. (2000) Maize profilin isoforms are functionally distinct. Plant Cell 12, 583598.
Lloyd, G. and McCown, B.H. (1981) Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot-tip culture. Proceedings of the International Plant Propagators' Society 30, 421427.
Macherel, D., Viale, A. and Akazawa, T. (1986) Protein phosphorylation in amyloplasts isolated from suspension-cultured cells of sycamore (Acer pseudoplatanus L.). Plant Physiology 80, 10411044.
Masters, C. (1984) Interactions between glycolytic enzymes and components of the cytomatrix. Journal of Cell Biology 99, 222S – 225S.
Masters, C. (1996) On the role of the cytoskeleton in metabolic compartmentation. pp. 130in Hesketh, J.E. (Ed.) The cytoskeleton, Volume 2 Role in cell physiology. Greenwich, Connecticut JAI Press.
Molecular, Probes Handbook. (2003) Available at website http://www.probes.com/handbook/sections/2003.html.
Moore, R. (1986) Cytochemical localization of calcium in cap cells of primary roots of Zea mays L. Journal of Experimental Botany 37, 7379.
Norenburg, J.L. and Barrett, J.M. (1987) Steedman's polyester wax embedment and de-embedment for combined light and scanning electron microscopy. Journal of Electron Microscopy Technique 6, 3541.
Pammenter, N.W. and Berjak, P. (1999) A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms. Seed Science Research 9, 1337.
Pammenter, N.W., Greggains, V., Kioko, J.I., Wesley-Smith, J., Berjak, P. and Finch-Savage, W.E. (1998) Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis. Seed Science Research 8, 463471.
Pammenter, N.W., Berjak, P., Wesley-Smith, J., Vander Willigen, C. (2002) Experimental aspects of drying and recovery. pp. 93110in Black, M.;, Pritchard, H.W.Desiccation and survival in plants: Drying without dying. Wallingford, CABI Publishing.
Perán, R., Pammenter, N.W., Naicker, J. and Berjak, P. (2004) The influence of rehydration technique on the response of recalcitrant seed embryos to desiccation. Seed Science Research 14, 179184.
Perbal, G., Driss-Ecole, D., Tewinkel, M. and Volkmann, D. (1997) Statocyte polarity and gravisensing in seedling roots grown in microgravity. Planta 203, (suppl.) S57S62.
Plieth, C. and Trewavas, A.J. (2002) Reorientation of seedlings in the earth's gravitational field induces cytosolic calcium transients. Plant Physiology 129, 786796.
Sack, F.D. (1991) Plant gravity sensing. International Review of Cytology 127, 193252.
Sinclair, W. and Trewavas, A.J. (1997) Calcium in gravitropism: a re-examination. Planta 203, (suppl.) S85S90.
Sinclair, W., Oliver, I., Maher, P. and Trewavas, A. (1996) The role of calmodulin in the gravitropic response of the Arabidopsis thaliana agr-3 mutant. Planta 199, 343351.
Slocum, R.D. and Roux, S.J. (1982) An improved method for the subcellular localization of calcium using a modification of the antimonate precipitation technique. Journal of Histochemistry and Cytochemistry 30, 617629.
Slocum, R.D. and Roux, S.J. (1983) Cellular and subcellular localization of calcium in gravistimulated oat coleoptiles and its possible significance in the establishment of tropic curvature. Planta 157, 481492.
Spurr, A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructural Research 26, 3143.
Trewavas, A. (1992) What remains of the Cholodny–Went theory? Plant Cell and Environment 15, 761794.
Volkmann, D., Baluška, F. (2000) Actin cytoskeleton related to gravisensing in higher plants. pp. 557571in Staiger, C.J.;, Baluška, F.;, Volkmann, D.;, Barlow, P. (Eds) Actin: A dynamic framework for multiple plant cell functions. Dordrecht, Kluwer Publishers.
Walters, C., Pammenter, N.W., Berjak, P. and Crane, J. (2001) Desiccation damage, accelerated ageing and respiration in desiccation tolerant and sensitive seeds. Seed Science Research 11, 135148.
Wesley-Smith, J., Walters, C., Pammenter, N.W. and Berjak, P. (2001) Interactions among water content, rapid (non-equilibrium) cooling to –196°C and survival of embryonic axes of Aesculus hippocastanum L. seeds. Cryobiology 42, 196206.
Winter, H. and Huber, S. (2000) Sucrose metabolism and the actin cytoskeleton: SuSy as actin-binding protein. pp. 119128in Staiger, C.J.;, Baluška, F.;, Volkmann, D.;, Barlow, P. (Eds) Actin: A dynamic framework for multiple plant cell functions. Dordrecht, Kluwer Publishers.
Winter, H., Huber, J.L. and Huber, S.C. (1997) Membrane association of sucrose synthase: changes during the graviresponse and possible control by protein phosphorylation. FEBS Letters 420, 151155.
Winter, H., Huber, J.L. and Huber, S.C. (1998) Identification of sucrose synthase as an actin-binding protein. FEBS Letters 430, 205208.
Wolfe, S.L. (1993) Molecular and cellular biology. Belmont, California Wadsworth.
Yoder, T.I., Zheng, H-Q., Todd, P. and Staehelin, L.A. (2001) Amyloplast sedimentation dynamics in maize columella cells support a new model for the gravity-sensing apparatus of roots. Plant Physiology 125, 10451060.
Yokota, E. and Shimmen, T. (2000) Characterization of native actin-binding proteins from pollen: myosin and the actin-bundling proteins, 135-ABP and 115-ABP. pp. 103118in Staiger, C.J.;, Baluška, F.;, Volkmann, D.;, Barlow, P. (Eds) Actin: A dynamic framework for multiple plant cell functions. Dordrecht, Kluwer Publishers.
Young, L.M. and Evans, M.L. (1994) Calcium-dependent asymmetric movement of 3H-indole-3-acetic acid across gravistimulated isolated root caps of maize. Plant Growth Regulation 14, 235242

Keywords

Calcium, with magnesium, is essential for normal seedling development from partially dehydrated recalcitrant axes: a study on Trichilia dregeana Sond.

  • Patricia Berjak (a1) and David Mycock (a2)

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