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Benzyladenine promotes early activation of p34cdc2-like kinase(s) during maize germination

Published online by Cambridge University Press:  22 February 2007

Ivonne Herrera-Teigeiro ,
Luis F. Jiménez-García  and
Jorge M. Vázquez-Ramos
Ivonne Herrera-Teigeiro
Affiliation:
Departamento de Bioquímica, Facultad de Química, México D.F. 04510, México
Luis F. Jiménez-García
Affiliation:
Departamento de Biología, Facultad de Ciencias, UNAM, Avenida Universidad y Copilco, México D.F. 04510, México.
Jorge M. Vázquez-Ramos*
Affiliation:
Departamento de Bioquímica, Facultad de Química, México D.F. 04510, México
*
*Correspondence Fax: 525 6225284 Email: jorman@servidor.unam.mx
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Abstract

The cell cycle is regulated, at least partially, by protein phosphorylation, activity that is carried out by a series of calcium-independent protein kinases among which the p34cdc2 kinase plays a preponderant role. The behaviour of p34cdc2-like protein kinase(s) has been followed during germination of maize in the presence or absence of the synthetic cytokinin benzyladenine (BA). Whereas there is an increase in p34cdc2-like kinase(s) activity at 15 h of germination in the presence of BA, coinciding with a peak of Ca2+-independent kinase activity, the amount of either the transcript for p34cdc2 or of the corresponding protein(s) does not show much variation during the 0–24 h period of germination studied, whether the phytohormone is present or not. Benzyladenine stimulates the cell cycle and promotes an early mitosis during maize germination. We have found evidence that BA promotes the movement of the p34cdc2-like protein(s) to nuclei several hours before this takes place during germination of control seeds, and this may constitute part of the mechanism by which the phytohormone promotes cytokinesis in plants.

Keywords

p34cdc2 kinasegerminationcytokininsmaize
Type
Research Article
Information
Seed Science Research , Volume 9 , Issue 1 , January 1999 , pp. 55 - 62
Copyright
Copyright © Cambridge University Press 1999

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References

Baiza, A.M., Vázquez-Ramos, J.M. and Sánchez de Jiménez, E. (1989) DNA synthesis and cell division in embryonic maize tissues during germination. Journal of Plant Physiology 135, 416–421.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1994) Seeds: Physiology of development and germination. (2nd edition) New York, Plenum Press.CrossRefGoogle Scholar
Blow, J.J. (1996) Eukaryotic DNA Replication. New York, IRL Press.CrossRefGoogle Scholar
Booher, R.N., Alfa, C.E., Hyams, J.S. and Beach, D.H. (1989) The fission yeast cdc2 / cdc13 / suc1 protein kinase: regulation of catalytic activity and nuclear location. Cell 58, 485–497.CrossRefGoogle Scholar
Cano, E. and Mahadevan, C. (1995) Parallel signal processing among mammalian MAPKs. Trends in Biochemical Sciences 20, 117–122.CrossRefGoogle ScholarPubMed
Coello, P. and Vázquez-Ramos, J.M.. (1995) Maize DNA polymerase 2 is a phosphoprotein with increasing activity during germination. European Journal of Biochemistry 231, 99–103.CrossRefGoogle ScholarPubMed
Colasanti, J., Tyers, M. and Sundaresan, V. (1991) Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays. Proceedings of the National Academy of Sciences USA 88, 3377–3381.CrossRefGoogle ScholarPubMed
Colasanti, J., Cho, S.O., Wick, S. and Sundaresan, V. (1993). Localization of the functional p34cdc2 homolog of maize in root tip and stomatal complex cells: association with predicted division sites. Plant Cell 5, 1101–1111.CrossRefGoogle ScholarPubMed
Cruz-García, F., Zúñiga-Aguilar, J.J. and Vázquez-Ramos, J.M. (1998) Effect of stimulating maize germination on cell cycle proteins. Physiologia Plantarum 102, 573–581.CrossRefGoogle Scholar
Durán, N., Bach, M., Puigdoménech, P. and Palau, J. (1984) Characterization of antigenic polypeptides of the RNP, Sm and SS-B nuclear antigens from calf thymus. Molecular Immunology 21, 731–736.CrossRefGoogle ScholarPubMed
Feiler, H.S. and Jacobs, T.W. (1990) Cell division in higher plants: A cdc2 gene, its 34 kDa product, and histone H1 kinase activity in peas. Proceedings of the National Academy of Sciences USA 87, 5397–5401.CrossRefGoogle Scholar
Fobert, P.R., Gaudin, V., Lunness, P., Coen, E.S. and Doonan, J.H. (1996) Distinct classes of cdc2-related genes are differentially expressed during the cell division cycle in plants. Plant Cell 8, 1465–1476.Google ScholarPubMed
Hunter, T. (1993) Breaking the cycle. Cell 75, 839–841.CrossRefGoogle Scholar
John, P.C.L., Sek, F.J. and Lee, M.G. (1989) A homolog of the cell cycle control protein p34cdc2 participates in the division cycle of Chlamydomonas, and a similar protein is detectable in higher plants and remote taxa. Plant Cell 1, 1185–1193.Google Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Lee, M.G. and Nurse, P. (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2+. Nature 327, 31–35.CrossRefGoogle ScholarPubMed
Magyar, Z., Mészáros, T., Miskolczi, P., Deák, M., Fehér, A., Brown, S., Kondorosi, E., Athanasiadis, A., Pongor, S., Bilgin, M., Bakó, L., Koncz, C. and Dudits, D. (1997) Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa seeds. Plant Cell 9, 223–235.Google Scholar
Morgan, D.O. (1997) Cyclin-dependent kinases: engines, clocks and microprocessors. Annual Reviews of Cell and Developmental Biology 13, 261–291.CrossRefGoogle ScholarPubMed
Nasheuer, H.P., Moore, A., Wahl, A.F. and Wang, T.S.F. (1991) Cell cycle dependent phosphorylation of human DNA polymerase a. Journal of Biological Chemistry 266, 7893–7903.CrossRefGoogle Scholar
Nurse, P. (1994) Ordering S phase and M phase in the cell cycle. Cell 79, 547–550.CrossRefGoogle Scholar
Pines, J. and Hunter, T. (1989) Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2. Cell 58, 833–846.CrossRefGoogle ScholarPubMed
Reed, S.I. and Wittemberg, C. (1990) A mitotic role for the cdc28 protein kinase of S. cerevisiae. Proceedings of the National Academy of Sciences USA 87, 5697–5701.CrossRefGoogle Scholar
Reed, S.I., Hadwiger, J.A. and Lorincz, A.T. (1985) Protein kinase activity associated with the product of the yeast cell division cycle gene cdc28. Proceedings of the National Academy of Sciences USA 77, 2119–2123.Google Scholar
Reyes-Jiménez, J., Jiménez-García, L.F., González, M.A. and Vázquez-Ramos, J.M. (1991) Benzyladenine stimulation of nuclear DNA synthesis and cell division in germinating maize. Seed Science Research 1, 113–117.CrossRefGoogle Scholar
Simanis, V. and Nurse, P. (1986) The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation. Cell 45, 261–268.CrossRefGoogle ScholarPubMed
Vázquez-Ramos, J.M. and Reyes, J. (1990) Stimulation of DNA synthesis and DNA polymerase activity by benzyladenine during early germination of maize axes. Canadian Journal of Botany 68, 2590–2594.CrossRefGoogle Scholar
Zhang, K., Letham, D.S. and John, P.C.L. (1996) Cytokinin controls the cell cycle at mitosis by stimulating the tyrosine dephosphorylation and activation of p34cdc2-like histone kinase. Planta 200, 2–12.CrossRefGoogle ScholarPubMed
Zúñiga-Aguilar, J.J., López, I., Gómez, A. and Vázquez-Ramos, J.M. (1995) Does benzyladenine stimulate DNA metabolism by modifying gene expression during maize germination? Seed Science Research 5, 219–226.CrossRefGoogle Scholar