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U2AF35 is encoded by an essential gene clustered in an operon with RRM/cyclophilin in Caenorhabditis elegans

Published online by Cambridge University Press:  01 April 1999

DIEGO A.R. ZORIO  and
THOMAS BLUMENTHAL
DIEGO A.R. ZORIO
Affiliation:
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
THOMAS BLUMENTHAL
Affiliation:
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
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Abstract

In most species the 3′ splice site is recognized initially by an interaction between the two-subunit splicing factor U2AF with the polypyrimidine (poly(Y)) tract that results in recruitment of the U2 snRNP to the branch-point consensus just upstream. In contrast, in Caenorhabditis elegans, both the poly(Y) tract and the branch-point consensus sequences are missing, apparently replaced by the highly conserved U4CAG/R 3′ splice site consensus. Nevertheless C. elegans U2AF65 is very similar to its mammalian and fly counterparts and may recognize the 3′ splice site consensus. Here we report the cloning of the C. elegans U2AF35 gene, uaf-2. We show that it lacks an identifiable RS domain, which, in flies, has been shown to play a role in RNA binding, but it contains an extended glycine-rich stretch at its C-terminus. uaf-2 is in an operon with cyp-13, a gene that encodes a cyclophilin with an RRM domain at its N-terminus. We demonstrate by RNA interference that both U2AF genes, uaf-1 (which encodes U2AF65) and uaf-2, are required for viability, whereas cyp-13 is apparently not.

Keywords

pre-mRNA processingRNA interferenceRNA splicingU2 snRNA auxiliary factor
Type
REPORT
Information
RNA , Volume 5 , Issue 4 , April 1999 , pp. 487 - 494
Copyright
© 1999 RNA Society

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