Skip to main content Accessibility help
×
Home

A simple and efficient method to reduce nontemplated nucleotide addition at the 3′ terminus of RNAs transcribed by T7 RNA polymerase

  • C. KAO (a1), M. ZHENG (a2) and S. RÜDISSER (a2)

Abstract

DNA templates modified with C2′-methoxyls at the last two nucleotides of the 5′ termini dramatically reduced nontemplated nucleotide addition by the T7 RNA polymerase from both single- and double-stranded DNA templates. This strategy was used to generate several different transcripts. Two of the transcripts were demonstrated by nuclear magnetic resonance spectroscopy to be unaffected in their sequence. Transcripts produced from the modified templates can be purified with greater ease and should be useful in a number of applications.

Copyright

Corresponding author

Reprint requests to: C. Cheng Kao, Department of Biology, 1001 East 3rd St., Indiana University, Bloomington, Indiana 47405-3700, USA; e-mail: ckao@bio.indiana.edu.

Keywords

Related content

Powered by UNSILO

A simple and efficient method to reduce nontemplated nucleotide addition at the 3′ terminus of RNAs transcribed by T7 RNA polymerase

  • C. KAO (a1), M. ZHENG (a2) and S. RÜDISSER (a2)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.